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Novel Anti-Microbial Metallic Surfaces for Infection Control / SARAH MARLEY

Swansea University Author: SARAH MARLEY

  • Redacted version - open access under embargo until: 31st August 2026

Abstract

Hospital acquired infections pose a significant risk to patients, particularly the elderly and those who are immunocompromised. With the emergence of highly resistant pathogens it is more important than ever to reduce the potential for transmission. This study investigates reducing the likelihood fo...

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Published: Swansea 2021
Institution: Swansea University
Degree level: Master of Research
Degree name: MSc by Research
Supervisor: Jenkins, Rowena E. ; Brown, M, R.
URI: https://cronfa.swan.ac.uk/Record/cronfa58300
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first_indexed 2021-10-12T11:16:47Z
last_indexed 2023-01-11T14:38:48Z
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spelling 2022-12-18T09:34:33.9998322 v2 58300 2021-10-12 Novel Anti-Microbial Metallic Surfaces for Infection Control a3caf7d2e4c4004132fa33af475626d4 SARAH MARLEY SARAH MARLEY true false 2021-10-12 Hospital acquired infections pose a significant risk to patients, particularly the elderly and those who are immunocompromised. With the emergence of highly resistant pathogens it is more important than ever to reduce the potential for transmission. This study investigates reducing the likelihood for surfaces to act as a reservoir of bacterial transmission in hospitals by exploring the feasibility of antimicrobial coatings. The four bacteria most commonly found in hospital acquired infections were chosen for efficacy experiments (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Klebsiella pneumoniae). Halloysite, naturally occurring aluminosilicate nanotubes, are biocompatible and have the ability to hold solutions within their lumen. These are hypothesised to be effective when incorporated into a coating as a slow release mechanism for disinfectants. Three disinfectants were chosen due to their chemical stability and approved contact safety for humans (Polyhexamethylene biguanide hydrochloride, Octenidine dihydrochloride and Chloroxylenol). The first portion of the study focused on bacterial adhesion and growth on steel discs, along with the minimum concentration of disinfectant required to inhibit cell growth. The second set of experiments looked at production of the disinfectant filled nanotubes and the resin coating on steel discs. The final experiments determined the antimicrobial efficacy of the nanotubes and coating on a qualitative level. The Gram-positive bacteria adhered more strongly to surfaces than the Gram-negative bacteria. Halloysite intercalated with chloroxylenol was effective at killing planktonic and plate-grown bacteria, whilst the remaining disinfectants were not. When incorporated into polyvinyl butyral resin, no disinfectant displayed antimicrobial properties. Immediate future work should focus on exploring the loading efficiency of the disinfectant and improving the coating method, along with testing various other disinfectants and polymers, considering porosity and particle dispersion during production. Overall, this study serves to successfully create a methodology for initial production of the coating and testing of its antimicrobial properties. E-Thesis Swansea Infection Control, Nanoreservoir, Surface, Coating, Anti-microbial, Disinfectant, Halloysite 12 10 2021 2021-10-12 A selection of third party content is redacted or is partially redacted from this thesis due to copyright restrictions. COLLEGE NANME COLLEGE CODE Swansea University Jenkins, Rowena E. ; Brown, M, R. Master of Research MSc by Research The European Social Fund (ESF) through the European Union’s Convergence programme administered by the Welsh Government 2022-12-18T09:34:33.9998322 2021-10-12T12:13:44.9130827 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised SARAH MARLEY 1 Under embargo Under embargo 2021-10-12T12:23:46.6033297 Output 3182664 application/pdf Redacted version - open access true 2026-08-31T00:00:00.0000000 Copyright: The author, Sarah Marley, 2021. true eng
title Novel Anti-Microbial Metallic Surfaces for Infection Control
spellingShingle Novel Anti-Microbial Metallic Surfaces for Infection Control
SARAH MARLEY
title_short Novel Anti-Microbial Metallic Surfaces for Infection Control
title_full Novel Anti-Microbial Metallic Surfaces for Infection Control
title_fullStr Novel Anti-Microbial Metallic Surfaces for Infection Control
title_full_unstemmed Novel Anti-Microbial Metallic Surfaces for Infection Control
title_sort Novel Anti-Microbial Metallic Surfaces for Infection Control
author_id_str_mv a3caf7d2e4c4004132fa33af475626d4
author_id_fullname_str_mv a3caf7d2e4c4004132fa33af475626d4_***_SARAH MARLEY
author SARAH MARLEY
author2 SARAH MARLEY
format E-Thesis
publishDate 2021
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 0
active_str 0
description Hospital acquired infections pose a significant risk to patients, particularly the elderly and those who are immunocompromised. With the emergence of highly resistant pathogens it is more important than ever to reduce the potential for transmission. This study investigates reducing the likelihood for surfaces to act as a reservoir of bacterial transmission in hospitals by exploring the feasibility of antimicrobial coatings. The four bacteria most commonly found in hospital acquired infections were chosen for efficacy experiments (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Klebsiella pneumoniae). Halloysite, naturally occurring aluminosilicate nanotubes, are biocompatible and have the ability to hold solutions within their lumen. These are hypothesised to be effective when incorporated into a coating as a slow release mechanism for disinfectants. Three disinfectants were chosen due to their chemical stability and approved contact safety for humans (Polyhexamethylene biguanide hydrochloride, Octenidine dihydrochloride and Chloroxylenol). The first portion of the study focused on bacterial adhesion and growth on steel discs, along with the minimum concentration of disinfectant required to inhibit cell growth. The second set of experiments looked at production of the disinfectant filled nanotubes and the resin coating on steel discs. The final experiments determined the antimicrobial efficacy of the nanotubes and coating on a qualitative level. The Gram-positive bacteria adhered more strongly to surfaces than the Gram-negative bacteria. Halloysite intercalated with chloroxylenol was effective at killing planktonic and plate-grown bacteria, whilst the remaining disinfectants were not. When incorporated into polyvinyl butyral resin, no disinfectant displayed antimicrobial properties. Immediate future work should focus on exploring the loading efficiency of the disinfectant and improving the coating method, along with testing various other disinfectants and polymers, considering porosity and particle dispersion during production. Overall, this study serves to successfully create a methodology for initial production of the coating and testing of its antimicrobial properties.
published_date 2021-10-12T04:14:43Z
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score 11.013082

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