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Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study

Zainab Khan, Gareth Healey Orcid Logo, Roberta Paravati, Nidhika Berry, Eugene Rees, Lavinia Margarit, Deya Gonzalez Orcid Logo, Simon Emery, Steve Conlan Orcid Logo

Frontiers in Cellular and Infection Microbiology, Volume: 11

Swansea University Authors: Gareth Healey Orcid Logo, Nidhika Berry, Lavinia Margarit, Deya Gonzalez Orcid Logo, Steve Conlan Orcid Logo

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DOI (Published version): 10.3389/fcimb.2021.752275

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Objectives: To investigate whether women with overactive bladder (OAB) symptoms and no evidence of clinical infection by conventional clean-catch midstream urine cultures have alternative indicators of sub-clinical infection.Patients/Subjects, Materials & Methods: The study was a prospective, bl...

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Published in: Frontiers in Cellular and Infection Microbiology
ISSN: 2235-2988
Published: Frontiers Media SA 2021
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2022-10-26T15:14:53.2483328</datestamp><bib-version>v2</bib-version><id>58079</id><entry>2021-09-24</entry><title>Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study</title><swanseaauthors><author><sid>5926519f89187489cfd5e1478aa188b1</sid><ORCID>0000-0001-9531-1220</ORCID><firstname>Gareth</firstname><surname>Healey</surname><name>Gareth Healey</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>d6cd8f38e7e704a0810ee95f6cc3e02f</sid><firstname>Nidhika</firstname><surname>Berry</surname><name>Nidhika Berry</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>1cdeff800fa264ed1424b0d86c587c67</sid><firstname>Lavinia</firstname><surname>Margarit</surname><name>Lavinia Margarit</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>bafdf635eb81280304eedf4b18e65d4e</sid><ORCID>0000-0002-1838-6752</ORCID><firstname>Deya</firstname><surname>Gonzalez</surname><name>Deya Gonzalez</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>0bb6bd247e32fb4249de62c0013b51cb</sid><ORCID>0000-0002-2562-3461</ORCID><firstname>Steve</firstname><surname>Conlan</surname><name>Steve Conlan</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2021-09-24</date><deptcode>PMSC</deptcode><abstract>Objectives: To investigate whether women with overactive bladder (OAB) symptoms and no evidence of clinical infection by conventional clean-catch midstream urine cultures have alternative indicators of sub-clinical infection.Patients/Subjects, Materials &amp; Methods: The study was a prospective, blinded case-control study with 147 participants recruited, including 73 OAB patients and 74 controls. The OAB group comprised female patients of at least 18 years of age who presented with OAB symptoms for more than 3 months. Clean-catch midstream urine samples were examined for pyuria by microscopy; subjected to routine and enhanced microbiological cultures and examined for the presence of 10 different cytokines, chemokines, and prostaglandins by ELISA.Results: The mean age and BMI of participants in both groups were similar. No significant difference in the number of women with pyuria was observed between OAB and control groups (p = 0.651). Routine laboratory cultures were positive in three (4%) of women in the OAB group, whereas the enhanced cultures isolated bacteria in 17 (23.2%) of the OAB patients. In the control group, no positive cultures were observed using routine laboratory cultures, whereas enhanced culture isolated bacteria in 8 (10.8%) patients. No significant differences were observed in the concentrations of PGE2, PGF2&#x3B1;, MCP-1, sCD40L, MIP-1&#x3B2;, IL12p70/p40, IL12/IL-23p40, IL-5, EGF and GRO-&#x3B1; between the OAB and control groups.Conclusions: Patients with OAB symptoms have significant bacterial growth on enhanced culture of the urine, which is often not detectable through routine culture, suggesting a subclinical infection. Enhanced culture techniques should therefore be used routinely for the effective diagnosis and management of OAB.</abstract><type>Journal Article</type><journal>Frontiers in Cellular and Infection Microbiology</journal><volume>11</volume><journalNumber/><paginationStart/><paginationEnd/><publisher>Frontiers Media SA</publisher><placeOfPublication/><isbnPrint/><isbnElectronic/><issnPrint/><issnElectronic>2235-2988</issnElectronic><keywords>overactive bladder syndrome, subclinical infection, enhanced culture, bacteria, midstream urine culture</keywords><publishedDay>30</publishedDay><publishedMonth>9</publishedMonth><publishedYear>2021</publishedYear><publishedDate>2021-09-30</publishedDate><doi>10.3389/fcimb.2021.752275</doi><url/><notes/><college>COLLEGE NANME</college><department>Medicine</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>PMSC</DepartmentCode><institution>Swansea University</institution><apcterm>External research funder(s) paid the OA fee (includes OA grants disbursed by the Library)</apcterm><funders>UKRI</funders><projectreference>MC_PC_19053</projectreference><lastEdited>2022-10-26T15:14:53.2483328</lastEdited><Created>2021-09-24T09:55:29.9883745</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Zainab</firstname><surname>Khan</surname><order>1</order></author><author><firstname>Gareth</firstname><surname>Healey</surname><orcid>0000-0001-9531-1220</orcid><order>2</order></author><author><firstname>Roberta</firstname><surname>Paravati</surname><order>3</order></author><author><firstname>Nidhika</firstname><surname>Berry</surname><order>4</order></author><author><firstname>Eugene</firstname><surname>Rees</surname><order>5</order></author><author><firstname>Lavinia</firstname><surname>Margarit</surname><order>6</order></author><author><firstname>Deya</firstname><surname>Gonzalez</surname><orcid>0000-0002-1838-6752</orcid><order>7</order></author><author><firstname>Simon</firstname><surname>Emery</surname><order>8</order></author><author><firstname>Steve</firstname><surname>Conlan</surname><orcid>0000-0002-2562-3461</orcid><order>9</order></author></authors><documents><document><filename>58079__21282__b13ea71445be45c9b3673dc48969ad3a.pdf</filename><originalFilename>58079.pdf</originalFilename><uploaded>2021-10-22T15:07:40.3377227</uploaded><type>Output</type><contentLength>372424</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>&#xA9; 2021 Khan, Healey, Paravati, Berry, Rees, Margarit, Gonzalez, Emery and Conlan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY)</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2022-10-26T15:14:53.2483328 v2 58079 2021-09-24 Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study 5926519f89187489cfd5e1478aa188b1 0000-0001-9531-1220 Gareth Healey Gareth Healey true false d6cd8f38e7e704a0810ee95f6cc3e02f Nidhika Berry Nidhika Berry true false 1cdeff800fa264ed1424b0d86c587c67 Lavinia Margarit Lavinia Margarit true false bafdf635eb81280304eedf4b18e65d4e 0000-0002-1838-6752 Deya Gonzalez Deya Gonzalez true false 0bb6bd247e32fb4249de62c0013b51cb 0000-0002-2562-3461 Steve Conlan Steve Conlan true false 2021-09-24 PMSC Objectives: To investigate whether women with overactive bladder (OAB) symptoms and no evidence of clinical infection by conventional clean-catch midstream urine cultures have alternative indicators of sub-clinical infection.Patients/Subjects, Materials & Methods: The study was a prospective, blinded case-control study with 147 participants recruited, including 73 OAB patients and 74 controls. The OAB group comprised female patients of at least 18 years of age who presented with OAB symptoms for more than 3 months. Clean-catch midstream urine samples were examined for pyuria by microscopy; subjected to routine and enhanced microbiological cultures and examined for the presence of 10 different cytokines, chemokines, and prostaglandins by ELISA.Results: The mean age and BMI of participants in both groups were similar. No significant difference in the number of women with pyuria was observed between OAB and control groups (p = 0.651). Routine laboratory cultures were positive in three (4%) of women in the OAB group, whereas the enhanced cultures isolated bacteria in 17 (23.2%) of the OAB patients. In the control group, no positive cultures were observed using routine laboratory cultures, whereas enhanced culture isolated bacteria in 8 (10.8%) patients. No significant differences were observed in the concentrations of PGE2, PGF2α, MCP-1, sCD40L, MIP-1β, IL12p70/p40, IL12/IL-23p40, IL-5, EGF and GRO-α between the OAB and control groups.Conclusions: Patients with OAB symptoms have significant bacterial growth on enhanced culture of the urine, which is often not detectable through routine culture, suggesting a subclinical infection. Enhanced culture techniques should therefore be used routinely for the effective diagnosis and management of OAB. Journal Article Frontiers in Cellular and Infection Microbiology 11 Frontiers Media SA 2235-2988 overactive bladder syndrome, subclinical infection, enhanced culture, bacteria, midstream urine culture 30 9 2021 2021-09-30 10.3389/fcimb.2021.752275 COLLEGE NANME Medicine COLLEGE CODE PMSC Swansea University External research funder(s) paid the OA fee (includes OA grants disbursed by the Library) UKRI MC_PC_19053 2022-10-26T15:14:53.2483328 2021-09-24T09:55:29.9883745 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Zainab Khan 1 Gareth Healey 0000-0001-9531-1220 2 Roberta Paravati 3 Nidhika Berry 4 Eugene Rees 5 Lavinia Margarit 6 Deya Gonzalez 0000-0002-1838-6752 7 Simon Emery 8 Steve Conlan 0000-0002-2562-3461 9 58079__21282__b13ea71445be45c9b3673dc48969ad3a.pdf 58079.pdf 2021-10-22T15:07:40.3377227 Output 372424 application/pdf Version of Record true © 2021 Khan, Healey, Paravati, Berry, Rees, Margarit, Gonzalez, Emery and Conlan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) true eng http://creativecommons.org/licenses/by/4.0/
title Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
spellingShingle Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
Gareth Healey
Nidhika Berry
Lavinia Margarit
Deya Gonzalez
Steve Conlan
title_short Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
title_full Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
title_fullStr Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
title_full_unstemmed Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
title_sort Chronic Urinary Infection in Overactive Bladder Syndrome: A Prospective, Blinded Case Control Study
author_id_str_mv 5926519f89187489cfd5e1478aa188b1
d6cd8f38e7e704a0810ee95f6cc3e02f
1cdeff800fa264ed1424b0d86c587c67
bafdf635eb81280304eedf4b18e65d4e
0bb6bd247e32fb4249de62c0013b51cb
author_id_fullname_str_mv 5926519f89187489cfd5e1478aa188b1_***_Gareth Healey
d6cd8f38e7e704a0810ee95f6cc3e02f_***_Nidhika Berry
1cdeff800fa264ed1424b0d86c587c67_***_Lavinia Margarit
bafdf635eb81280304eedf4b18e65d4e_***_Deya Gonzalez
0bb6bd247e32fb4249de62c0013b51cb_***_Steve Conlan
author Gareth Healey
Nidhika Berry
Lavinia Margarit
Deya Gonzalez
Steve Conlan
author2 Zainab Khan
Gareth Healey
Roberta Paravati
Nidhika Berry
Eugene Rees
Lavinia Margarit
Deya Gonzalez
Simon Emery
Steve Conlan
format Journal article
container_title Frontiers in Cellular and Infection Microbiology
container_volume 11
publishDate 2021
institution Swansea University
issn 2235-2988
doi_str_mv 10.3389/fcimb.2021.752275
publisher Frontiers Media SA
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Objectives: To investigate whether women with overactive bladder (OAB) symptoms and no evidence of clinical infection by conventional clean-catch midstream urine cultures have alternative indicators of sub-clinical infection.Patients/Subjects, Materials & Methods: The study was a prospective, blinded case-control study with 147 participants recruited, including 73 OAB patients and 74 controls. The OAB group comprised female patients of at least 18 years of age who presented with OAB symptoms for more than 3 months. Clean-catch midstream urine samples were examined for pyuria by microscopy; subjected to routine and enhanced microbiological cultures and examined for the presence of 10 different cytokines, chemokines, and prostaglandins by ELISA.Results: The mean age and BMI of participants in both groups were similar. No significant difference in the number of women with pyuria was observed between OAB and control groups (p = 0.651). Routine laboratory cultures were positive in three (4%) of women in the OAB group, whereas the enhanced cultures isolated bacteria in 17 (23.2%) of the OAB patients. In the control group, no positive cultures were observed using routine laboratory cultures, whereas enhanced culture isolated bacteria in 8 (10.8%) patients. No significant differences were observed in the concentrations of PGE2, PGF2α, MCP-1, sCD40L, MIP-1β, IL12p70/p40, IL12/IL-23p40, IL-5, EGF and GRO-α between the OAB and control groups.Conclusions: Patients with OAB symptoms have significant bacterial growth on enhanced culture of the urine, which is often not detectable through routine culture, suggesting a subclinical infection. Enhanced culture techniques should therefore be used routinely for the effective diagnosis and management of OAB.
published_date 2021-09-30T04:14:19Z
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