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Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment

Dorottya Nagy, Clare M. C. Gillis, Katie Davies, Abigail L. Fowden, Paul Rees Orcid Logo, John W. Wills, Katherine Hughes

Communications Biology, Volume: 4, Issue: 1

Swansea University Author: Paul Rees Orcid Logo

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DOI (Published version): 10.1038/s42003-021-02502-6

Abstract

The human breast and ovine mammary gland undergo striking levels of postnatal development, leading to formation of terminal duct lobular units (TDLUs). Here we interrogate aspects of sheep TDLU growth as a model of breast development and to increase understanding of ovine mammogenesis. The distribut...

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Published in: Communications Biology
ISSN: 2399-3642
Published: Springer Science and Business Media LLC 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa57721
first_indexed 2021-09-01T08:08:53Z
last_indexed 2023-01-11T14:37:49Z
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spelling 2022-07-07T13:47:45.9341557 v2 57721 2021-08-31 Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment 537a2fe031a796a3bde99679ee8c24f5 0000-0002-7715-6914 Paul Rees Paul Rees true false 2021-08-31 EAAS The human breast and ovine mammary gland undergo striking levels of postnatal development, leading to formation of terminal duct lobular units (TDLUs). Here we interrogate aspects of sheep TDLU growth as a model of breast development and to increase understanding of ovine mammogenesis. The distributions of epithelial nuclear Ki67 positivity differ significantly between younger and older lambs. Ki67 expression is polarised to the leading edge of the developing TDLUs. Intraepithelial ductal macrophages exhibit periodicity and considerably increased density in lambs approaching puberty. Stromal macrophages are more abundant centrally than peripherally. Intraepithelial T lymphocytes are more numerous in older lambs. Stromal hotspots of Ki67 expression colocalize with immune cell aggregates that exhibit distinct organisation consistent with tertiary lymphoid structures. The lamb mammary gland thus exhibits a dynamic mucosal and stromal immune microenvironment and constitutes a valuable model system that provides new insights into postnatal breast development. Journal Article Communications Biology 4 1 Springer Science and Business Media LLC 2399-3642 Developmental biology, Image processing, Innate immunity, Lymphoid tissues,Machine learning 20 8 2021 2021-08-20 10.1038/s42003-021-02502-6 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University Another institution paid the OA fee This work was supported by a grant from the British Veterinary Association Animal Welfare Foundation Norman Hayward Fund awarded to KH [grant number NHF_2016_03_KH]. JWW is grateful to Girton College and the University of Cambridge Herchel-Smith Fund for supporting him with Fellowships. The authors would like to acknowledge the UK Engineering and Physical Sciences Research Council (grant EP/N013506/1), and the UK Biotechnology and Biological Sciences Research Council (grant number BB/P026818/1), both awarded to PR, for supporting the work. 2022-07-07T13:47:45.9341557 2021-08-31T08:51:39.4274867 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Dorottya Nagy 1 Clare M. C. Gillis 2 Katie Davies 3 Abigail L. Fowden 4 Paul Rees 0000-0002-7715-6914 5 John W. Wills 6 Katherine Hughes 7 57721__20728__672ee93ac4064739a5d9b0c26129edc6.pdf 57721.pdf 2021-09-01T09:08:21.4308796 Output 8638659 application/pdf Version of Record true © The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/
title Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
spellingShingle Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
Paul Rees
title_short Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
title_full Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
title_fullStr Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
title_full_unstemmed Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
title_sort Developing ovine mammary terminal duct lobular units have a dynamic mucosal and stromal immune microenvironment
author_id_str_mv 537a2fe031a796a3bde99679ee8c24f5
author_id_fullname_str_mv 537a2fe031a796a3bde99679ee8c24f5_***_Paul Rees
author Paul Rees
author2 Dorottya Nagy
Clare M. C. Gillis
Katie Davies
Abigail L. Fowden
Paul Rees
John W. Wills
Katherine Hughes
format Journal article
container_title Communications Biology
container_volume 4
container_issue 1
publishDate 2021
institution Swansea University
issn 2399-3642
doi_str_mv 10.1038/s42003-021-02502-6
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description The human breast and ovine mammary gland undergo striking levels of postnatal development, leading to formation of terminal duct lobular units (TDLUs). Here we interrogate aspects of sheep TDLU growth as a model of breast development and to increase understanding of ovine mammogenesis. The distributions of epithelial nuclear Ki67 positivity differ significantly between younger and older lambs. Ki67 expression is polarised to the leading edge of the developing TDLUs. Intraepithelial ductal macrophages exhibit periodicity and considerably increased density in lambs approaching puberty. Stromal macrophages are more abundant centrally than peripherally. Intraepithelial T lymphocytes are more numerous in older lambs. Stromal hotspots of Ki67 expression colocalize with immune cell aggregates that exhibit distinct organisation consistent with tertiary lymphoid structures. The lamb mammary gland thus exhibits a dynamic mucosal and stromal immune microenvironment and constitutes a valuable model system that provides new insights into postnatal breast development.
published_date 2021-08-20T20:04:30Z
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