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Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912

Bethan Kultschar, Ed Dudley, Steve Wilson, Carole Llewellyn

Microorganisms, Volume: 9, Issue: 5, Start page: 910

Swansea University Authors: Ed Dudley, Carole Llewellyn

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DOI (Published version): 10.3390/microorganisms9050910

Abstract

Ultraviolet A (UV-A) is the major component of UV radiation reaching the Earth’s surface, causing indirect damage to photosynthetic organisms via the production of reactive oxygen species (ROS). In comparison, UV-B causes both direct damage to biomolecules and indirect damage. UV-B is well studied i...

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Published in: Microorganisms
ISSN: 2076-2607
Published: MDPI AG 2021
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URI: https://cronfa.swan.ac.uk/Record/cronfa56752
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spelling 2022-07-08T11:13:01.9791828 v2 56752 2021-04-28 Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912 c7d05f992a817cd3b9a5f946bd909b71 Ed Dudley Ed Dudley true false bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 2021-04-28 BMS Ultraviolet A (UV-A) is the major component of UV radiation reaching the Earth’s surface, causing indirect damage to photosynthetic organisms via the production of reactive oxygen species (ROS). In comparison, UV-B causes both direct damage to biomolecules and indirect damage. UV-B is well studied in cyanobacterial research due to their long evolutionary history and adaptation to high levels of UV, with less work on the effects of UV-A. In this study, the response of key metabolites in Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR, 15 µmol·m−2·s−1) supplemented with UV-A (11 µmol·m−2·s−1) was investigated using gas chromatography- mass spectrometry (GC-MS). Results showed an overall significant increase in metabolite levels up to 24 h of UV-A exposure. Compared with previously reported UV-B (PAR + UV-B) and PAR only results, UV-A showed more similarity compared to PAR only exposure as opposed to supplemented UV-B. The amino acids glutamate, phenylalanine and leucine showed differences in levels between UV (both supplemented UV-A and supplemented UV-B) and PAR only (non-supplemented PAR), hinting to their relevance in UV stress response. The fatty acids, palmitic and stearic acid, showed positive log2 fold-change (FC) in supplemented UV-A and PAR only experiments but negative log2 FC in UV-B, indicating the more harmful effect of UV-B on primary metabolism. Less research has been conducted on UV-A exposure and cyanobacteria, a potential environmental stimuli for the optimisation of metabolites for industrial biotechnology. This study will add to the literature and knowledge on UV-A stress response at the metabolite level in cyanobacteria, especially within the less well-known species C. fritschii. Journal Article Microorganisms 9 5 910 MDPI AG 2076-2607 Cyanobacteria; C. fritschii; GC-MS; metabolites; metabolomics; ultraviolet radiation; biotechnology 24 4 2021 2021-04-24 10.3390/microorganisms9050910 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University Biotechnology and Biological Sciences Research Council (BBSRC iCASE studentship), UK; grant number BB/N503630/1. 2022-07-08T11:13:01.9791828 2021-04-28T09:57:00.1547694 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Bethan Kultschar 1 Ed Dudley 2 Steve Wilson 3 Carole Llewellyn 4 56752__19904__51be43cdaf23492fa93ff9c68f45330a.pdf 56752.pdf 2021-05-14T16:22:05.9593588 Output 1659346 application/pdf Version of Record true © 2021 by the authors. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY) license true eng https://creativecommons.org/licenses/by/4.0/
title Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
spellingShingle Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
Ed Dudley
Carole Llewellyn
title_short Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_full Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_fullStr Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_full_unstemmed Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_sort Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912
author_id_str_mv c7d05f992a817cd3b9a5f946bd909b71
bcd94bda79ebf4c2c82d82dfb027a140
author_id_fullname_str_mv c7d05f992a817cd3b9a5f946bd909b71_***_Ed Dudley
bcd94bda79ebf4c2c82d82dfb027a140_***_Carole Llewellyn
author Ed Dudley
Carole Llewellyn
author2 Bethan Kultschar
Ed Dudley
Steve Wilson
Carole Llewellyn
format Journal article
container_title Microorganisms
container_volume 9
container_issue 5
container_start_page 910
publishDate 2021
institution Swansea University
issn 2076-2607
doi_str_mv 10.3390/microorganisms9050910
publisher MDPI AG
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Ultraviolet A (UV-A) is the major component of UV radiation reaching the Earth’s surface, causing indirect damage to photosynthetic organisms via the production of reactive oxygen species (ROS). In comparison, UV-B causes both direct damage to biomolecules and indirect damage. UV-B is well studied in cyanobacterial research due to their long evolutionary history and adaptation to high levels of UV, with less work on the effects of UV-A. In this study, the response of key metabolites in Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR, 15 µmol·m−2·s−1) supplemented with UV-A (11 µmol·m−2·s−1) was investigated using gas chromatography- mass spectrometry (GC-MS). Results showed an overall significant increase in metabolite levels up to 24 h of UV-A exposure. Compared with previously reported UV-B (PAR + UV-B) and PAR only results, UV-A showed more similarity compared to PAR only exposure as opposed to supplemented UV-B. The amino acids glutamate, phenylalanine and leucine showed differences in levels between UV (both supplemented UV-A and supplemented UV-B) and PAR only (non-supplemented PAR), hinting to their relevance in UV stress response. The fatty acids, palmitic and stearic acid, showed positive log2 fold-change (FC) in supplemented UV-A and PAR only experiments but negative log2 FC in UV-B, indicating the more harmful effect of UV-B on primary metabolism. Less research has been conducted on UV-A exposure and cyanobacteria, a potential environmental stimuli for the optimisation of metabolites for industrial biotechnology. This study will add to the literature and knowledge on UV-A stress response at the metabolite level in cyanobacteria, especially within the less well-known species C. fritschii.
published_date 2021-04-24T04:11:57Z
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