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Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912

Carole Llewellyn, Carolyn Greig, Alla Silkina Orcid Logo, Bethan Kultschar, Matthew Hitchings Orcid Logo, Garry Farnham

Scientific Reports, Volume: 10, Issue: 1

Swansea University Authors: Carole Llewellyn, Carolyn Greig, Alla Silkina Orcid Logo, Matthew Hitchings Orcid Logo

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DOI (Published version): 10.1038/s41598-020-77402-6

Abstract

The “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring t...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: Springer Science and Business Media LLC 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa55786
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However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. 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spelling 2021-01-14T13:39:21.4414918 v2 55786 2020-11-30 Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912 bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 7f95061328bd6726f7472ced9cb8d937 Carolyn Greig Carolyn Greig true false 216d36449db09ed98c6971a2254a2457 0000-0002-1804-8083 Alla Silkina Alla Silkina true false be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 2020-11-30 FGSEN The “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation. Journal Article Scientific Reports 10 1 Springer Science and Business Media LLC 2045-2322 1 12 2020 2020-12-01 10.1038/s41598-020-77402-6 COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2021-01-14T13:39:21.4414918 2020-11-30T16:15:29.1546571 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Carole Llewellyn 1 Carolyn Greig 2 Alla Silkina 0000-0002-1804-8083 3 Bethan Kultschar 4 Matthew Hitchings 0000-0002-5527-4709 5 Garry Farnham 6 55786__19062__154a7615374d44c7993c1a19b093d9f9.pdf 55786.pdf 2021-01-14T13:38:10.4983147 Output 3672810 application/pdf Version of Record true © The Author(s) 2020. Tis article is licensed under a Creative Commons Attribution 4.0 International License true eng http://creativecommons.org/licenses/by/4.0/ 34 Carole Llewellyn 0000-0003-1545-5190 C.A.Llewellyn@Swansea.ac.uk true NCBI BioProject with Accession Number PRJNA545395. true
title Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
spellingShingle Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
Carole Llewellyn
Carolyn Greig
Alla Silkina
Matthew Hitchings
title_short Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_full Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_fullStr Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_full_unstemmed Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
title_sort Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912
author_id_str_mv bcd94bda79ebf4c2c82d82dfb027a140
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be98847c72c14a731c4a6b7bc02b3bcf
author_id_fullname_str_mv bcd94bda79ebf4c2c82d82dfb027a140_***_Carole Llewellyn
7f95061328bd6726f7472ced9cb8d937_***_Carolyn Greig
216d36449db09ed98c6971a2254a2457_***_Alla Silkina
be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
author Carole Llewellyn
Carolyn Greig
Alla Silkina
Matthew Hitchings
author2 Carole Llewellyn
Carolyn Greig
Alla Silkina
Bethan Kultschar
Matthew Hitchings
Garry Farnham
format Journal article
container_title Scientific Reports
container_volume 10
container_issue 1
publishDate 2020
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-020-77402-6
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description The “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation.
published_date 2020-12-01T04:10:15Z
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