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Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model
,
Luke Roberts,
Carla Carisi,
Alwena Morgan ,
Rowan Brown ,
Jeffrey Davies
Current Protocols in Neuroscience, Volume: 94, Issue: 1
Swansea University Authors:
Daniel Rees , Luke Roberts, Carla Carisi, Alwena Morgan , Rowan Brown , Jeffrey Davies
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DOI (Published version): 10.1002/cpns.105
Abstract
Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identif...
| Published in: | Current Protocols in Neuroscience |
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| ISSN: | 1934-8584 1934-8576 |
| Published: |
Wiley
2020
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| Online Access: |
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa55596 |
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| Abstract: |
Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open‐access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high‐content imaging (HCI), and the subsequent open‐source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone‐based in vitro Parkinson's disease model. |
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| Keywords: |
fragmentation, image analysis, mitochondria, Parkinson's disease |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Funders: |
Wiley TA; This work was funded by grants from the Medical Research Council (Grant no. G0902250), Parkinson's UK, St David's Medical Foundation, Coleg Cymraeg Cenedlaethol PhD Studentship, and a BRACE PhD Studentship. |
| Issue: |
1 |