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Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker

Rob Driessen, Feihu Zhao Orcid Logo, Sandra Hofmann, Carlijn Bouten, Cecilia Sahlgren, Oscar Stassen

Micromachines, Volume: 11, Issue: 6, Start page: 552

Swansea University Author: Feihu Zhao Orcid Logo

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DOI (Published version): 10.3390/mi11060552

Abstract

Endothelial cells sense and respond to shear stress. Different in vitro model systems have been used to study the cellular responses to shear stress, but these platforms do not allow studies on high numbers of cells under uniform and controllable shear stress. The annular dish, or dish-in-a-dish (Di...

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Published in: Micromachines
ISSN: 2072-666X
Published: MDPI AG 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa54857
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first_indexed 2020-08-03T13:37:09Z
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spelling 2020-09-17T15:42:06.1639745 v2 54857 2020-08-03 Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker 1c6e79b6edd08c88a8d17a241cd78630 0000-0003-0515-6808 Feihu Zhao Feihu Zhao true false 2020-08-03 MEDE Endothelial cells sense and respond to shear stress. Different in vitro model systems have been used to study the cellular responses to shear stress, but these platforms do not allow studies on high numbers of cells under uniform and controllable shear stress. The annular dish, or dish-in-a-dish (DiaD), on the orbital shaker has been proposed as an accessible system to overcome these challenges. However, the influence of the DiaD design and the experimental parameters on the shear stress patterns is not known. In this study, we characterize different designs and experimental parameters (orbit size, speed and fluid height) using computational fluid dynamics. We optimize the DiaD for an atheroprotective flow, combining high shear stress levels with a low oscillatory shear index (OSI). We find that orbit size determines the DiaD design and parameters. The shear stress levels increase with increasing rotational speed and fluid height. Based on our optimization, we experimentally compare the 134/56 DiaD with regular dishes for cellular alignment and KLF2, eNOS, CDH2 and MCP1 expression. The calculated OSI has a strong impact on alignment and gene expression, emphasizing the importance of characterizing shear profiles in orbital setups. Journal Article Micromachines 11 6 552 MDPI AG 2072-666X shear stress; computational fluid dynamics; endothelial cells; orbital shaker; flow 29 5 2020 2020-05-29 10.3390/mi11060552 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-09-17T15:42:06.1639745 2020-08-03T14:35:25.1958237 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Rob Driessen 1 Feihu Zhao 0000-0003-0515-6808 2 Sandra Hofmann 3 Carlijn Bouten 4 Cecilia Sahlgren 5 Oscar Stassen 6 54857__17835__c0b6e72574e442128d64645152ab91cb.pdf 54857.pdf 2020-08-03T14:36:53.4008737 Output 3950281 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY). true English https://creativecommons.org/licenses/by/4.0/
title Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
spellingShingle Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
Feihu Zhao
title_short Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
title_full Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
title_fullStr Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
title_full_unstemmed Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
title_sort Computational Characterization of the Dish-In-A-Dish, A High Yield Culture Platform for Endothelial Shear Stress Studies on the Orbital Shaker
author_id_str_mv 1c6e79b6edd08c88a8d17a241cd78630
author_id_fullname_str_mv 1c6e79b6edd08c88a8d17a241cd78630_***_Feihu Zhao
author Feihu Zhao
author2 Rob Driessen
Feihu Zhao
Sandra Hofmann
Carlijn Bouten
Cecilia Sahlgren
Oscar Stassen
format Journal article
container_title Micromachines
container_volume 11
container_issue 6
container_start_page 552
publishDate 2020
institution Swansea University
issn 2072-666X
doi_str_mv 10.3390/mi11060552
publisher MDPI AG
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description Endothelial cells sense and respond to shear stress. Different in vitro model systems have been used to study the cellular responses to shear stress, but these platforms do not allow studies on high numbers of cells under uniform and controllable shear stress. The annular dish, or dish-in-a-dish (DiaD), on the orbital shaker has been proposed as an accessible system to overcome these challenges. However, the influence of the DiaD design and the experimental parameters on the shear stress patterns is not known. In this study, we characterize different designs and experimental parameters (orbit size, speed and fluid height) using computational fluid dynamics. We optimize the DiaD for an atheroprotective flow, combining high shear stress levels with a low oscillatory shear index (OSI). We find that orbit size determines the DiaD design and parameters. The shear stress levels increase with increasing rotational speed and fluid height. Based on our optimization, we experimentally compare the 134/56 DiaD with regular dishes for cellular alignment and KLF2, eNOS, CDH2 and MCP1 expression. The calculated OSI has a strong impact on alignment and gene expression, emphasizing the importance of characterizing shear profiles in orbital setups.
published_date 2020-05-29T04:08:39Z
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score 11.01753

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