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A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide

Sebastian Riedle, John W. Wills, Michelle Miniter, Don E. Otter, Harjinder Singh, Andy P. Brown, Stuart Micklethwaite, Paul Rees Orcid Logo, Ravin Jugdaohsingh, Nicole C. Roy, Rachel E. Hewitt, Jonathan J. Powell

Small, Volume: 16, Issue: 21, Start page: 2000486

Swansea University Author: Paul Rees Orcid Logo

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DOI (Published version): 10.1002/smll.202000486

Abstract

Human exposure to persistent, nonbiological nanoparticles and microparticles via the oral route is continuous and large scale (1012–1013 particles per day per adult in Europe). Whether this matters or not is unknown but confirmed health risks with airborne particle exposure warns against complacency...

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Published in: Small
ISSN: 1613-6810 1613-6829
Published: Wiley 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa54222
first_indexed 2020-05-14T19:08:28Z
last_indexed 2020-06-04T19:09:37Z
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spelling 2020-06-04T18:45:39.1599618 v2 54222 2020-05-14 A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide 537a2fe031a796a3bde99679ee8c24f5 0000-0002-7715-6914 Paul Rees Paul Rees true false 2020-05-14 EAAS Human exposure to persistent, nonbiological nanoparticles and microparticles via the oral route is continuous and large scale (1012–1013 particles per day per adult in Europe). Whether this matters or not is unknown but confirmed health risks with airborne particle exposure warns against complacency. Murine models of oral exposure will help to identify risk but, to date, lack validation or relevance to humans. This work addresses that gap. It reports i) on a murine diet, modified with differing concentrations of the common dietary particle, food grade titanium dioxide (fgTiO2), an additive of polydisperse form that contains micro‐ and nano‐particles, ii) that these diets deliver particles to basal cells of intestinal lymphoid follicles, exactly as is reported as a “normal occurrence” in humans, iii) that confocal reflectance microscopy is the method of analytical choice to determine this, and iv) that food intake, weight gain, and Peyer's patch immune cell profiles, up to 18 weeks of feeding, do not differ between fgTiO2‐fed groups or controls. These findings afford a human‐relevant and validated oral dosing protocol for fgTiO2 risk assessment as well as provide a generalized platform for application to oral exposure studies with nano‐ and micro‐particles. Journal Article Small 16 21 2000486 Wiley 1613-6810 1613-6829 26 5 2020 2020-05-26 10.1002/smll.202000486 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2020-06-04T18:45:39.1599618 2020-05-14T13:37:36.8918253 Sebastian Riedle 1 John W. Wills 2 Michelle Miniter 3 Don E. Otter 4 Harjinder Singh 5 Andy P. Brown 6 Stuart Micklethwaite 7 Paul Rees 0000-0002-7715-6914 8 Ravin Jugdaohsingh 9 Nicole C. Roy 10 Rachel E. Hewitt 11 Jonathan J. Powell 12 54222__17233__7175f29ae8954aa0bfe4897233674663.pdf 54222.pdf 2020-05-14T13:39:30.0960560 Output 2278990 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution-NonCommercial-NoDerivs License (CC-BY-NC-ND). true English http://creativecommons.org/licenses/by-nc-nd/4.0/
title A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
spellingShingle A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
Paul Rees
title_short A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
title_full A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
title_fullStr A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
title_full_unstemmed A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
title_sort A Murine Oral‐Exposure Model for Nano‐ and Micro‐Particulates: Demonstrating Human Relevance with Food‐Grade Titanium Dioxide
author_id_str_mv 537a2fe031a796a3bde99679ee8c24f5
author_id_fullname_str_mv 537a2fe031a796a3bde99679ee8c24f5_***_Paul Rees
author Paul Rees
author2 Sebastian Riedle
John W. Wills
Michelle Miniter
Don E. Otter
Harjinder Singh
Andy P. Brown
Stuart Micklethwaite
Paul Rees
Ravin Jugdaohsingh
Nicole C. Roy
Rachel E. Hewitt
Jonathan J. Powell
format Journal article
container_title Small
container_volume 16
container_issue 21
container_start_page 2000486
publishDate 2020
institution Swansea University
issn 1613-6810
1613-6829
doi_str_mv 10.1002/smll.202000486
publisher Wiley
document_store_str 1
active_str 0
description Human exposure to persistent, nonbiological nanoparticles and microparticles via the oral route is continuous and large scale (1012–1013 particles per day per adult in Europe). Whether this matters or not is unknown but confirmed health risks with airborne particle exposure warns against complacency. Murine models of oral exposure will help to identify risk but, to date, lack validation or relevance to humans. This work addresses that gap. It reports i) on a murine diet, modified with differing concentrations of the common dietary particle, food grade titanium dioxide (fgTiO2), an additive of polydisperse form that contains micro‐ and nano‐particles, ii) that these diets deliver particles to basal cells of intestinal lymphoid follicles, exactly as is reported as a “normal occurrence” in humans, iii) that confocal reflectance microscopy is the method of analytical choice to determine this, and iv) that food intake, weight gain, and Peyer's patch immune cell profiles, up to 18 weeks of feeding, do not differ between fgTiO2‐fed groups or controls. These findings afford a human‐relevant and validated oral dosing protocol for fgTiO2 risk assessment as well as provide a generalized platform for application to oral exposure studies with nano‐ and micro‐particles.
published_date 2020-05-26T19:59:30Z
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