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Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)

Stefan Pfuhler, Jan van Benthem, Rodger Curren, shareen Doak, Maria Dusinska, Makoto Hayashi, Robert H. Heflich, Darren Kidd, David Kirkland, Yang Luan, Gladys Ouedraogo, Kerstin Reisinger, Toshio Sofuni, Frédérique van Acker, Ying Yang, Raffaella Corvi

Mutation Research: Genetic Toxicology and Environmental Mutagenesis, Volume: 850-851, Start page: 503135

Swansea University Author: shareen Doak

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DOI (Published version): 10.1016/j.mrgentox.2020.503135

Abstract

Use of three-dimensional (3D) tissue equivalents in toxicology has been increasing over the last decade as novel preclinical test systems and as alternatives to animal testing. In the area of genetic toxicology, progress has been made with establishing robust protocols for skin, airway (lung) and li...

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Published in: Mutation Research: Genetic Toxicology and Environmental Mutagenesis
ISSN: 1383-5718 1879-3592
Published: Elsevier BV 2020
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URI: https://cronfa.swan.ac.uk/Record/cronfa53559
first_indexed 2020-02-17T20:34:55Z
last_indexed 2025-03-14T03:38:55Z
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spelling 2025-03-12T10:15:30.4589517 v2 53559 2020-02-17 Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT) 8f70286908f67238a527a98cbf66d387 shareen Doak shareen Doak true false 2020-02-17 Use of three-dimensional (3D) tissue equivalents in toxicology has been increasing over the last decade as novel preclinical test systems and as alternatives to animal testing. In the area of genetic toxicology, progress has been made with establishing robust protocols for skin, airway (lung) and liver tissue equivalents. In light of these advancements, a “Use of 3D Tissues in Genotoxicity Testing” working group (WG) met at the 7th IWGT meeting in Tokyo in November 2017 to discuss progress with these models and how they may fit into a genotoxicity testing strategy. The workshop demonstrated that skin models have reached an advanced state of validation following over 10 years of development, while liver and airway model-based genotoxicity assays show promise but are at an early stage of development. Further effort in liver and airway model-based assays is needed to address the lack of coverage of the three main endpoints of genotoxicity (mutagenicity, clastogenicity and aneugenicity), and information on metabolic competence. The IWGT WG believes that the 3D skin comet and micronucleus assays are now sufficiently validated to undergo an independent peer review of the validation study, followed by development of individual OECD Test Guidelines. Journal Article Mutation Research: Genetic Toxicology and Environmental Mutagenesis 850-851 503135 Elsevier BV 1383-5718 1879-3592 In vitro genotoxicity testing; DNA damage; Comet assay; Micronucleus test; Reconstructed human skin; Liver spheroids; 3D airway models 1 2 2020 2020-02-01 10.1016/j.mrgentox.2020.503135 COLLEGE NANME COLLEGE CODE Swansea University Another institution paid the OA fee The 3D skin comet and micronucleus validation outcome presented at IWGT was financially supported by the German Federal Ministry for Research and Technology (grant Nos. 0315226, 0316008) and by Cosmetics Europe, Belgium. The research on CeO2 nanoparticles presented in chapter 2b was financially supported by funds from TNO, The Netherlands. 2025-03-12T10:15:30.4589517 2020-02-17T16:16:31.0890404 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Biomedical Science Stefan Pfuhler 1 Jan van Benthem 2 Rodger Curren 3 shareen Doak 4 Maria Dusinska 5 Makoto Hayashi 6 Robert H. Heflich 7 Darren Kidd 8 David Kirkland 9 Yang Luan 10 Gladys Ouedraogo 11 Kerstin Reisinger 12 Toshio Sofuni 13 Frédérique van Acker 14 Ying Yang 15 Raffaella Corvi 16 53559__33792__c8406f66f7d042a88b6b4d6aed615077.pdf 53559.VOR.pdf 2025-03-12T10:06:51.3565917 Output 2001583 application/pdf Version of Record true © 2020 The Authors. This is an open access article under the CC BY-NC-ND license. true eng http://creativecommons.org/licenses/by-nc-nd/4.0/
title Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
spellingShingle Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
shareen Doak
title_short Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
title_full Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
title_fullStr Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
title_full_unstemmed Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
title_sort Use of in vitro 3D tissue models in genotoxicity testing: Strategic fit, validation status and way forward. Report of the working group from the 7th International Workshop on Genotoxicity Testing (IWGT)
author_id_str_mv 8f70286908f67238a527a98cbf66d387
author_id_fullname_str_mv 8f70286908f67238a527a98cbf66d387_***_shareen Doak
author shareen Doak
author2 Stefan Pfuhler
Jan van Benthem
Rodger Curren
shareen Doak
Maria Dusinska
Makoto Hayashi
Robert H. Heflich
Darren Kidd
David Kirkland
Yang Luan
Gladys Ouedraogo
Kerstin Reisinger
Toshio Sofuni
Frédérique van Acker
Ying Yang
Raffaella Corvi
format Journal article
container_title Mutation Research: Genetic Toxicology and Environmental Mutagenesis
container_volume 850-851
container_start_page 503135
publishDate 2020
institution Swansea University
issn 1383-5718
1879-3592
doi_str_mv 10.1016/j.mrgentox.2020.503135
publisher Elsevier BV
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Biomedical Science{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Biomedical Science
document_store_str 1
active_str 0
description Use of three-dimensional (3D) tissue equivalents in toxicology has been increasing over the last decade as novel preclinical test systems and as alternatives to animal testing. In the area of genetic toxicology, progress has been made with establishing robust protocols for skin, airway (lung) and liver tissue equivalents. In light of these advancements, a “Use of 3D Tissues in Genotoxicity Testing” working group (WG) met at the 7th IWGT meeting in Tokyo in November 2017 to discuss progress with these models and how they may fit into a genotoxicity testing strategy. The workshop demonstrated that skin models have reached an advanced state of validation following over 10 years of development, while liver and airway model-based genotoxicity assays show promise but are at an early stage of development. Further effort in liver and airway model-based assays is needed to address the lack of coverage of the three main endpoints of genotoxicity (mutagenicity, clastogenicity and aneugenicity), and information on metabolic competence. The IWGT WG believes that the 3D skin comet and micronucleus assays are now sufficiently validated to undergo an independent peer review of the validation study, followed by development of individual OECD Test Guidelines.
published_date 2020-02-01T04:46:24Z
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score 11.089551

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