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Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium

Mariam Raliou, Doulaye Dembélé, Anna Düvel, Philippe Bolifraud, Julie Aubert, Tristan Mary-Huard, Dominique Rocha, François Piumi, Sophie Mockly, Maike Heppelmann, Isabelle Dieuzy-Labaye, Peter Zieger, David G. E. Smith, Hans-Joachim Schuberth, Iain Martin Sheldon, Olivier Sandra, Martin Sheldon Orcid Logo

PLOS ONE, Volume: 14, Issue: 8, Start page: e0220244

Swansea University Author: Martin Sheldon Orcid Logo

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DOI (Published version): 10.1371/journal.pone.0220244

Abstract

Cattle with subclinical endometritis (SCE) are sub-fertile and diagnosing subclinical uterine disease remains a challenge. The hypothesis for this study was that endometrial inflammation is reflected in mRNA expression patterns of peripheral blood leucocytes. Transcriptome profiles were evaluated in...

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Published in: PLOS ONE
ISSN: 1932-6203
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa51324
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first_indexed 2019-08-09T16:32:02Z
last_indexed 2019-08-16T21:30:04Z
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Transcriptome profiles were evaluated in healthy cows and in cows with SCE using circulating white blood cells (WBC) and endometrial biopsy samples collected from the same animals at 45&#x2013;55 days postpartum. Bioinformatic analyses of microarray-based transcriptional data identified gene profiles associated with distinct biological functions in circulating WBC and endometrium. In circulating WBC, SCE promotes a pro-inflammatory environment, whereas functions related to tissue remodeling are also affected in the endometrium. Nineteen differentially expressed genes associated with SCE were common to both circulating WBC and the endometrium. Among these genes, transcript abundance of immune factors C3, C2, LTF, PF4 and TRAPPC13 were up-regulated in SCE cows at 45&#x2013;55 days postpartum. Moreover, mRNA expression of C3, CXCL8, LTF, TLR2 and TRAPPC13 was temporally regulated during the postpartum period in circulating WBC of healthy cows compared with SCE cows. This observation might indicate an advantageous modulation of the immune system in healthy animals. The transcript abundance of these genes represents a potential source of indicators for postpartum uterine health.</abstract><type>Journal Article</type><journal>PLOS ONE</journal><volume>14</volume><journalNumber>8</journalNumber><paginationStart>e0220244</paginationStart><publisher/><issnElectronic>1932-6203</issnElectronic><keywords/><publishedDay>2</publishedDay><publishedMonth>8</publishedMonth><publishedYear>2019</publishedYear><publishedDate>2019-08-02</publishedDate><doi>10.1371/journal.pone.0220244</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><degreesponsorsfunders>BBSRC</degreesponsorsfunders><apcterm/><lastEdited>2019-08-16T14:40:27.8002956</lastEdited><Created>2019-08-06T10:10:29.4421104</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Mariam</firstname><surname>Raliou</surname><order>1</order></author><author><firstname>Doulaye</firstname><surname>Demb&#xE9;l&#xE9;</surname><order>2</order></author><author><firstname>Anna</firstname><surname>D&#xFC;vel</surname><order>3</order></author><author><firstname>Philippe</firstname><surname>Bolifraud</surname><order>4</order></author><author><firstname>Julie</firstname><surname>Aubert</surname><order>5</order></author><author><firstname>Tristan</firstname><surname>Mary-Huard</surname><order>6</order></author><author><firstname>Dominique</firstname><surname>Rocha</surname><order>7</order></author><author><firstname>Fran&#xE7;ois</firstname><surname>Piumi</surname><order>8</order></author><author><firstname>Sophie</firstname><surname>Mockly</surname><order>9</order></author><author><firstname>Maike</firstname><surname>Heppelmann</surname><order>10</order></author><author><firstname>Isabelle</firstname><surname>Dieuzy-Labaye</surname><order>11</order></author><author><firstname>Peter</firstname><surname>Zieger</surname><order>12</order></author><author><firstname>David</firstname><surname>G. 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Smith</surname><order>13</order></author><author><firstname>Hans-Joachim</firstname><surname>Schuberth</surname><order>14</order></author><author><firstname>Iain Martin</firstname><surname>Sheldon</surname><order>15</order></author><author><firstname>Olivier</firstname><surname>Sandra</surname><order>16</order></author><author><firstname>Martin</firstname><surname>Sheldon</surname><orcid>0000-0001-7902-5558</orcid><order>17</order></author></authors><documents><document><filename>0051324-06082019101104.pdf</filename><originalFilename>journal.pone.0220244.pdf</originalFilename><uploaded>2019-08-06T10:11:04.1970000</uploaded><type>Output</type><contentLength>1998925</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-08-02T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2019-08-16T14:40:27.8002956 v2 51324 2019-08-06 Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium ab0f74b794e59cc270c69e63ee1d9748 0000-0001-7902-5558 Martin Sheldon Martin Sheldon true false 2019-08-06 BMS Cattle with subclinical endometritis (SCE) are sub-fertile and diagnosing subclinical uterine disease remains a challenge. The hypothesis for this study was that endometrial inflammation is reflected in mRNA expression patterns of peripheral blood leucocytes. Transcriptome profiles were evaluated in healthy cows and in cows with SCE using circulating white blood cells (WBC) and endometrial biopsy samples collected from the same animals at 45–55 days postpartum. Bioinformatic analyses of microarray-based transcriptional data identified gene profiles associated with distinct biological functions in circulating WBC and endometrium. In circulating WBC, SCE promotes a pro-inflammatory environment, whereas functions related to tissue remodeling are also affected in the endometrium. Nineteen differentially expressed genes associated with SCE were common to both circulating WBC and the endometrium. Among these genes, transcript abundance of immune factors C3, C2, LTF, PF4 and TRAPPC13 were up-regulated in SCE cows at 45–55 days postpartum. Moreover, mRNA expression of C3, CXCL8, LTF, TLR2 and TRAPPC13 was temporally regulated during the postpartum period in circulating WBC of healthy cows compared with SCE cows. This observation might indicate an advantageous modulation of the immune system in healthy animals. The transcript abundance of these genes represents a potential source of indicators for postpartum uterine health. Journal Article PLOS ONE 14 8 e0220244 1932-6203 2 8 2019 2019-08-02 10.1371/journal.pone.0220244 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University BBSRC 2019-08-16T14:40:27.8002956 2019-08-06T10:10:29.4421104 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Mariam Raliou 1 Doulaye Dembélé 2 Anna Düvel 3 Philippe Bolifraud 4 Julie Aubert 5 Tristan Mary-Huard 6 Dominique Rocha 7 François Piumi 8 Sophie Mockly 9 Maike Heppelmann 10 Isabelle Dieuzy-Labaye 11 Peter Zieger 12 David G. E. Smith 13 Hans-Joachim Schuberth 14 Iain Martin Sheldon 15 Olivier Sandra 16 Martin Sheldon 0000-0001-7902-5558 17 0051324-06082019101104.pdf journal.pone.0220244.pdf 2019-08-06T10:11:04.1970000 Output 1998925 application/pdf Version of Record true 2019-08-02T00:00:00.0000000 Released under the terms of a Creative Commons Attribution License (CC-BY). true eng
title Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
spellingShingle Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
Martin Sheldon
title_short Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
title_full Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
title_fullStr Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
title_full_unstemmed Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
title_sort Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium
author_id_str_mv ab0f74b794e59cc270c69e63ee1d9748
author_id_fullname_str_mv ab0f74b794e59cc270c69e63ee1d9748_***_Martin Sheldon
author Martin Sheldon
author2 Mariam Raliou
Doulaye Dembélé
Anna Düvel
Philippe Bolifraud
Julie Aubert
Tristan Mary-Huard
Dominique Rocha
François Piumi
Sophie Mockly
Maike Heppelmann
Isabelle Dieuzy-Labaye
Peter Zieger
David G. E. Smith
Hans-Joachim Schuberth
Iain Martin Sheldon
Olivier Sandra
Martin Sheldon
format Journal article
container_title PLOS ONE
container_volume 14
container_issue 8
container_start_page e0220244
publishDate 2019
institution Swansea University
issn 1932-6203
doi_str_mv 10.1371/journal.pone.0220244
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Cattle with subclinical endometritis (SCE) are sub-fertile and diagnosing subclinical uterine disease remains a challenge. The hypothesis for this study was that endometrial inflammation is reflected in mRNA expression patterns of peripheral blood leucocytes. Transcriptome profiles were evaluated in healthy cows and in cows with SCE using circulating white blood cells (WBC) and endometrial biopsy samples collected from the same animals at 45–55 days postpartum. Bioinformatic analyses of microarray-based transcriptional data identified gene profiles associated with distinct biological functions in circulating WBC and endometrium. In circulating WBC, SCE promotes a pro-inflammatory environment, whereas functions related to tissue remodeling are also affected in the endometrium. Nineteen differentially expressed genes associated with SCE were common to both circulating WBC and the endometrium. Among these genes, transcript abundance of immune factors C3, C2, LTF, PF4 and TRAPPC13 were up-regulated in SCE cows at 45–55 days postpartum. Moreover, mRNA expression of C3, CXCL8, LTF, TLR2 and TRAPPC13 was temporally regulated during the postpartum period in circulating WBC of healthy cows compared with SCE cows. This observation might indicate an advantageous modulation of the immune system in healthy animals. The transcript abundance of these genes represents a potential source of indicators for postpartum uterine health.
published_date 2019-08-02T04:03:10Z
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