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Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity

Emily Preedy Orcid Logo, Stefano Perni, Polina Prokopovich

Colloids and Surfaces B: Biointerfaces, Volume: 157, Pages: 146 - 156

Swansea University Author: Emily Preedy Orcid Logo

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DOI (Published version): 10.1016/j.colsurfb.2017.05.019

Abstract

Bone cells are damaged by wear particles originating from total joint replacement implants. We investigated Mesenchymal stem cells (MSCs) nanomechanical properties when exposed to cobalt and titanium nanoparticles (resembling wear debris) of different sizes for up to 3 days using AFM nanoindentation...

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Published in: Colloids and Surfaces B: Biointerfaces
ISSN: 0927-7765
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa51204
first_indexed 2019-07-25T17:48:22Z
last_indexed 2019-08-09T16:31:36Z
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spelling 2019-08-07T11:51:13.5596336 v2 51204 2019-07-25 Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity a4ae2d29d6b017b303c85efa3a9503d0 0000-0003-0377-6747 Emily Preedy Emily Preedy true false 2019-07-25 EAAS Bone cells are damaged by wear particles originating from total joint replacement implants. We investigated Mesenchymal stem cells (MSCs) nanomechanical properties when exposed to cobalt and titanium nanoparticles (resembling wear debris) of different sizes for up to 3 days using AFM nanoindentation; along with flow-cytometry and MTT assay. The results demonstrated that cells exposed to increasing concentrations of nanoparticles had a lower value of elasticity and spring constant without significant effect on cell metabolic activity and viability but some morphological alteration (bleeping). Cobalt induced greater effects than titanium and this is consistent with the general knowledge of cyto-compatibility of the later.This work demonstrates for the first time that metal nanoparticles do not only influence MSCs enzymes activity but also cell structure; however, they do not result in full membrane damage. Furthermore, the mechanical changes are concentration and particles composition dependent but little influenced by the particle size. Journal Article Colloids and Surfaces B: Biointerfaces 157 146 156 0927-7765 Mesenchimal stem cells, Wear particles, Nanomechanical properties, AFM, Titanium nanoparticles, Cobalt nanoparticles 1 9 2017 2017-09-01 10.1016/j.colsurfb.2017.05.019 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2019-08-07T11:51:13.5596336 2019-07-25T14:14:36.5947687 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Emily Preedy 0000-0003-0377-6747 1 Stefano Perni 2 Polina Prokopovich 3
title Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
spellingShingle Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
Emily Preedy
title_short Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
title_full Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
title_fullStr Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
title_full_unstemmed Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
title_sort Cobalt and titanium nanoparticles influence on mesenchymal stem cell elasticity and turgidity
author_id_str_mv a4ae2d29d6b017b303c85efa3a9503d0
author_id_fullname_str_mv a4ae2d29d6b017b303c85efa3a9503d0_***_Emily Preedy
author Emily Preedy
author2 Emily Preedy
Stefano Perni
Polina Prokopovich
format Journal article
container_title Colloids and Surfaces B: Biointerfaces
container_volume 157
container_start_page 146
publishDate 2017
institution Swansea University
issn 0927-7765
doi_str_mv 10.1016/j.colsurfb.2017.05.019
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering
document_store_str 0
active_str 0
description Bone cells are damaged by wear particles originating from total joint replacement implants. We investigated Mesenchymal stem cells (MSCs) nanomechanical properties when exposed to cobalt and titanium nanoparticles (resembling wear debris) of different sizes for up to 3 days using AFM nanoindentation; along with flow-cytometry and MTT assay. The results demonstrated that cells exposed to increasing concentrations of nanoparticles had a lower value of elasticity and spring constant without significant effect on cell metabolic activity and viability but some morphological alteration (bleeping). Cobalt induced greater effects than titanium and this is consistent with the general knowledge of cyto-compatibility of the later.This work demonstrates for the first time that metal nanoparticles do not only influence MSCs enzymes activity but also cell structure; however, they do not result in full membrane damage. Furthermore, the mechanical changes are concentration and particles composition dependent but little influenced by the particle size.
published_date 2017-09-01T04:49:44Z
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score 11.04748

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