Journal article 243 views
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton
Journal of Colloid and Interface Science, Volume: 531, Pages: 410 - 420
Swansea University Author: Emily Preedy
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DOI (Published version): 10.1016/j.jcis.2018.07.028
Abstract
We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investiga...
Published in: | Journal of Colloid and Interface Science |
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ISSN: | 0021-9797 |
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2018
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URI: | https://cronfa.swan.ac.uk/Record/cronfa51202 |
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2019-08-09T16:31:36Z |
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2019-08-07T11:39:16.4623141 v2 51202 2019-07-25 Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton a4ae2d29d6b017b303c85efa3a9503d0 0000-0003-0377-6747 Emily Preedy Emily Preedy true false 2019-07-25 EAAS We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells.Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining. Journal Article Journal of Colloid and Interface Science 531 410 420 0021-9797 Osteoblast, Cobalt, Titanium, Nanoparticles, Wear debris 1 12 2018 2018-12-01 10.1016/j.jcis.2018.07.028 COLLEGE NANME Engineering and Applied Sciences School COLLEGE CODE EAAS Swansea University 2019-08-07T11:39:16.4623141 2019-07-25T14:10:49.7258022 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemical Engineering Stefano Perni 1 Lirong Yang 2 Emily Preedy 0000-0003-0377-6747 3 Polina Prokopovich 4 |
title |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
spellingShingle |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton Emily Preedy |
title_short |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
title_full |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
title_fullStr |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
title_full_unstemmed |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
title_sort |
Cobalt and Titanium nanoparticles influence on human osteoblast mitochondrial activity and biophysical properties of their cytoskeleton |
author_id_str_mv |
a4ae2d29d6b017b303c85efa3a9503d0 |
author_id_fullname_str_mv |
a4ae2d29d6b017b303c85efa3a9503d0_***_Emily Preedy |
author |
Emily Preedy |
author2 |
Stefano Perni Lirong Yang Emily Preedy Polina Prokopovich |
format |
Journal article |
container_title |
Journal of Colloid and Interface Science |
container_volume |
531 |
container_start_page |
410 |
publishDate |
2018 |
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Swansea University |
issn |
0021-9797 |
doi_str_mv |
10.1016/j.jcis.2018.07.028 |
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Faculty of Science and Engineering |
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facultyofscienceandengineering |
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Faculty of Science and Engineering |
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facultyofscienceandengineering |
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Faculty of Science and Engineering |
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School of Engineering and Applied Sciences - Chemical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemical Engineering |
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description |
We investigated the biophysical effects (cell elasticity and spring constant) caused on Saos-2 human osteoblast-like cells by nanosized metal (Co and Ti) wear debris, as well as the adhesive characteristics of cells after exposure to the metal nanoparticles. Cell mitochondrial activity was investigated using the MTT assays; along with LDH assay, metal uptake, cell apoptosis and mineralisation output (alizarin red assay) of the cells.Osteoblasts mitochondrial activity was not affected by Ti nanoparticles at concentrations up to 1 mg/ml and by Cobalt nanoparticles at concentrations < 0.5 mg/ml; however elasticity and spring constant were significantly modified by the exposure to nanoparticles of these metals in agreement with the alteration of cell conformation (shape), as result of the exposure to simulated wear debris, demonstrated by fluorescence images after actin staining. |
published_date |
2018-12-01T04:49:44Z |
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1821379641594609664 |
score |
11.04748 |