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Effects of cryopreservation on viability and functional stability of an industrially relevant alga

Steve Slocombe Orcid Logo, Rahul Kapoore Orcid Logo, María Huete-Ortega, John G. Day, Katarzyna Okurowska, Stephen P. Slocombe, Michele S. Stanley, Seetharaman Vaidyanathan

Scientific Reports, Volume: 9, Issue: 1

Swansea University Authors: Steve Slocombe Orcid Logo, Rahul Kapoore Orcid Logo

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DOI (Published version): 10.1038/s41598-019-38588-6

Abstract

As algal biotechnology develops, there is an increasing requirement to conserve cultures without the cost, time and genetic stability implications of conventional serial transfers, including issues regarding potential loss by failure to regrow, contamination on transfer, mix up and/or errors in the...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: Springer Science and Business Media LLC 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa49792
first_indexed 2019-03-29T12:39:42Z
last_indexed 2024-11-14T11:59:20Z
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spelling 2024-01-22T12:36:51.9076240 v2 49792 2019-03-28 Effects of cryopreservation on viability and functional stability of an industrially relevant alga 4a1ea486a78ed357efdfa053a277ae40 0000-0002-3549-7999 Steve Slocombe Steve Slocombe true false 5583be4600daecd670edac16f6e77e88 0000-0002-2287-0619 Rahul Kapoore Rahul Kapoore true false 2019-03-28 BGPS As algal biotechnology develops, there is an increasing requirement to conserve cultures without the cost, time and genetic stability implications of conventional serial transfers, including issues regarding potential loss by failure to regrow, contamination on transfer, mix up and/or errors in the documentation on transfer. Furthermore, it is crucial to ensure both viability and functionality are retained by stored stock-cultures. Low temperature storage, ranging from the use of domestic freezers to storage under liquid nitrogen, is widely being used, but the implication to stability and function rarely investigated. We report for the first time, retention of functionality in the maintenance of master stock-cultures of an industrially relevant, lipid-producing alga, under a variety of cryopreservation regimes. Storage in domestic (-15 °C), or conventional -80 °C freezers was suboptimal, with a rapid reduction in viability observed for samples at -15 °C and a >50% loss of viability, within one month, for samples stored at -80 °C. No reduction in viability occurred at -196 °C. Post-thaw culture functional performance was also influenced by the cryopreservation approach employed. Only samples held at -196 °C responded to nitrogen limitation in terms of growth characteristics and biochemical profiles (lipid production and chlorophyll a) comparable to the untreated control, cultured prior to cryopreservation. These results have important implications in microbial biotechnology, especially for those responsible for the conservation of genetic resources. Journal Article Scientific Reports 9 1 Springer Science and Business Media LLC 2045-2322 1 12 2019 2019-12-01 10.1038/s41598-019-38588-6 COLLEGE NANME Biosciences Geography and Physics School COLLEGE CODE BGPS Swansea University 2024-01-22T12:36:51.9076240 2019-03-28T15:46:27.8449039 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Steve Slocombe 0000-0002-3549-7999 1 Rahul Kapoore 0000-0002-2287-0619 2 María Huete-Ortega 3 John G. Day 4 Katarzyna Okurowska 5 Stephen P. Slocombe 6 Michele S. Stanley 7 Seetharaman Vaidyanathan 8 0049792-04042019151354.pdf 49792.pdf 2019-04-04T15:13:54.9370000 Output 2221268 application/pdf Version of Record true 2019-04-03T00:00:00.0000000 Released under the terms of a Creative Commons Attribution 4.0 International License (CC-BY). true eng
title Effects of cryopreservation on viability and functional stability of an industrially relevant alga
spellingShingle Effects of cryopreservation on viability and functional stability of an industrially relevant alga
Steve Slocombe
Rahul Kapoore
title_short Effects of cryopreservation on viability and functional stability of an industrially relevant alga
title_full Effects of cryopreservation on viability and functional stability of an industrially relevant alga
title_fullStr Effects of cryopreservation on viability and functional stability of an industrially relevant alga
title_full_unstemmed Effects of cryopreservation on viability and functional stability of an industrially relevant alga
title_sort Effects of cryopreservation on viability and functional stability of an industrially relevant alga
author_id_str_mv 4a1ea486a78ed357efdfa053a277ae40
5583be4600daecd670edac16f6e77e88
author_id_fullname_str_mv 4a1ea486a78ed357efdfa053a277ae40_***_Steve Slocombe
5583be4600daecd670edac16f6e77e88_***_Rahul Kapoore
author Steve Slocombe
Rahul Kapoore
author2 Steve Slocombe
Rahul Kapoore
María Huete-Ortega
John G. Day
Katarzyna Okurowska
Stephen P. Slocombe
Michele S. Stanley
Seetharaman Vaidyanathan
format Journal article
container_title Scientific Reports
container_volume 9
container_issue 1
publishDate 2019
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-019-38588-6
publisher Springer Science and Business Media LLC
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
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description As algal biotechnology develops, there is an increasing requirement to conserve cultures without the cost, time and genetic stability implications of conventional serial transfers, including issues regarding potential loss by failure to regrow, contamination on transfer, mix up and/or errors in the documentation on transfer. Furthermore, it is crucial to ensure both viability and functionality are retained by stored stock-cultures. Low temperature storage, ranging from the use of domestic freezers to storage under liquid nitrogen, is widely being used, but the implication to stability and function rarely investigated. We report for the first time, retention of functionality in the maintenance of master stock-cultures of an industrially relevant, lipid-producing alga, under a variety of cryopreservation regimes. Storage in domestic (-15 °C), or conventional -80 °C freezers was suboptimal, with a rapid reduction in viability observed for samples at -15 °C and a >50% loss of viability, within one month, for samples stored at -80 °C. No reduction in viability occurred at -196 °C. Post-thaw culture functional performance was also influenced by the cryopreservation approach employed. Only samples held at -196 °C responded to nitrogen limitation in terms of growth characteristics and biochemical profiles (lipid production and chlorophyll a) comparable to the untreated control, cultured prior to cryopreservation. These results have important implications in microbial biotechnology, especially for those responsible for the conservation of genetic resources.
published_date 2019-12-01T04:46:20Z
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