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Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells

Lleucu B Davies, Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

Tissue Engineering Part C: Methods

Swansea University Authors: Ruth Jones Orcid Logo, Cathy Thornton Orcid Logo

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DOI (Published version): 10.1089/ten.TEC.2019.0008

Abstract

The umbilical cord offers a source of readily available mesenchymal stromal cells for use in research and ultimately therapeutic application. However, methods of isolating these cells vary between investigators, and no standard method has been adopted. The aims of this work were to i) develop a meth...

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Published in: Tissue Engineering Part C: Methods
ISSN: 1937-3384 1937-3392
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa49644
first_indexed 2019-03-20T13:59:11Z
last_indexed 2019-04-09T13:04:15Z
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spelling 2019-04-08T15:49:08.4063235 v2 49644 2019-03-20 Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells a1a281c8720685c422892ef168d4b279 0000-0001-5811-8827 Ruth Jones Ruth Jones true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2019-03-20 MEDS The umbilical cord offers a source of readily available mesenchymal stromal cells for use in research and ultimately therapeutic application. However, methods of isolating these cells vary between investigators, and no standard method has been adopted. The aims of this work were to i) develop a methodology for the isolation of umbilical cord matrix cells without the use of enzymatic digestion or complicated dissection; ii) investigate the use of pooled maternal serum as a media supplement; and iii) to demonstrate that the cells isolated were mesenchymal stromal cells. We have demonstrated that incubating tissue explants of less than 2mm3 in serum for an hour, followed by the gradual addition of serum containing culture medium can increase cell yield compared to incubation in serum containing culture medium alone. More importantly, our method demonstrated that the use of pooled serum from women > 37 weeks pregnant (pooled maternal serum) yields higher cell numbers than the use of fetal bovine serum or pooled umbilical cord serum. Irrespective of the type of serum used the isolated cells were mesenchymal stromal cells according to the minimal criteria set out by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy. In conclusion, maternal serum has the potential to be used as an alternative to FBS for isolation and expansion of umbilical cord MSCs for clinical purposes. Journal Article Tissue Engineering Part C: Methods 1937-3384 1937-3392 stem cells, 22 2 2019 2019-02-22 10.1089/ten.TEC.2019.0008 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2019-04-08T15:49:08.4063235 2019-03-20T11:21:11.3858025 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Lleucu B Davies 1 Ruth Jones 0000-0001-5811-8827 2 Cathy Thornton 0000-0002-5153-573X 3 0049644-20032019112143.pdf MaternalSerumasaMSCIsolationToolFINALCompleteManuscript.pdf 2019-03-20T11:21:43.5500000 Output 14216791 application/pdf Accepted Manuscript true 2020-02-20T00:00:00.0000000 true eng
title Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
spellingShingle Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
Ruth Jones
Cathy Thornton
title_short Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
title_full Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
title_fullStr Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
title_full_unstemmed Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
title_sort Maternal serum, an isolation and expansion tool for umbilical cord matrix mesenchymal stromal cells
author_id_str_mv a1a281c8720685c422892ef168d4b279
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv a1a281c8720685c422892ef168d4b279_***_Ruth Jones
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author Ruth Jones
Cathy Thornton
author2 Lleucu B Davies
Ruth Jones
Cathy Thornton
format Journal article
container_title Tissue Engineering Part C: Methods
publishDate 2019
institution Swansea University
issn 1937-3384
1937-3392
doi_str_mv 10.1089/ten.TEC.2019.0008
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description The umbilical cord offers a source of readily available mesenchymal stromal cells for use in research and ultimately therapeutic application. However, methods of isolating these cells vary between investigators, and no standard method has been adopted. The aims of this work were to i) develop a methodology for the isolation of umbilical cord matrix cells without the use of enzymatic digestion or complicated dissection; ii) investigate the use of pooled maternal serum as a media supplement; and iii) to demonstrate that the cells isolated were mesenchymal stromal cells. We have demonstrated that incubating tissue explants of less than 2mm3 in serum for an hour, followed by the gradual addition of serum containing culture medium can increase cell yield compared to incubation in serum containing culture medium alone. More importantly, our method demonstrated that the use of pooled serum from women > 37 weeks pregnant (pooled maternal serum) yields higher cell numbers than the use of fetal bovine serum or pooled umbilical cord serum. Irrespective of the type of serum used the isolated cells were mesenchymal stromal cells according to the minimal criteria set out by the Mesenchymal and Tissue Stem Cell Committee of the International Society for Cellular Therapy. In conclusion, maternal serum has the potential to be used as an alternative to FBS for isolation and expansion of umbilical cord MSCs for clinical purposes.
published_date 2019-02-22T04:46:00Z
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score 11.3749895

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