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Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy

Nan Jian, Miriam Dowle, Richard D Horniblow, Chris Tselepis, Richard Palmer Orcid Logo

Nanotechnology, Volume: 27, Issue: 46, Start page: 46LT02

Swansea University Author: Richard Palmer Orcid Logo

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DOI (Published version): 10.1088/0957-4484/27/46/46lt02

Abstract

As the major iron storage protein, ferritin stores and releases iron for maintaining the balance of iron in fauna, flora, and bacteria. We present an investigation of the morphology and iron loading of ferritin (from equine spleen) using aberration-corrected high angle annular dark field scanning tr...

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Published in: Nanotechnology
ISSN: 0957-4484 1361-6528
Published: IOP Publishing 2016
Online Access: Check full text

URI: https://cronfa.swan.ac.uk/Record/cronfa49232
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first_indexed 2019-03-18T20:01:26Z
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spelling 2020-10-19T17:30:17.3932910 v2 49232 2019-03-18 Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy 6ae369618efc7424d9774377536ea519 0000-0001-8728-8083 Richard Palmer Richard Palmer true false 2019-03-18 MECH As the major iron storage protein, ferritin stores and releases iron for maintaining the balance of iron in fauna, flora, and bacteria. We present an investigation of the morphology and iron loading of ferritin (from equine spleen) using aberration-corrected high angle annular dark field scanning transmission electron microscopy. Atom counting method, with size selected Au clusters as mass standards, was employed to determine the number of iron atoms in the nanoparticle core of each ferritin protein. Quantitative analysis shows that the nuclearity of iron atoms in the mineral core varies from a few hundred iron atoms to around 5000 atoms. Moreover, a relationship between the iron loading and iron core morphology is established, in which mineral core nucleates from a single nanoparticle, then grows along the protein shell before finally forming either a solid or hollow core structure. Journal Article Nanotechnology 27 46 46LT02 IOP Publishing 0957-4484 1361-6528 18 11 2016 2016-11-18 10.1088/0957-4484/27/46/46lt02 COLLEGE NANME Mechanical Engineering COLLEGE CODE MECH Swansea University 2020-10-19T17:30:17.3932910 2019-03-18T14:28:14.9321128 Faculty of Science and Engineering School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Mechanical Engineering Nan Jian 1 Miriam Dowle 2 Richard D Horniblow 3 Chris Tselepis 4 Richard Palmer 0000-0001-8728-8083 5 49232__18458__29938688ccba46d980ead3ad40fa1e84.pdf Jian_2016_Nanotechnology_27_46LT02.pdf 2020-10-19T17:29:03.1984230 Output 2155933 application/pdf Version of Record true Distributed under the terms of a Creative Commons Attribution 4.0 (CC-BY) Licence. true eng https://creativecommons.org/licenses/by/4.0/
title Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
spellingShingle Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
Richard Palmer
title_short Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
title_full Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
title_fullStr Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
title_full_unstemmed Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
title_sort Morphology of the ferritin iron core by aberration corrected scanning transmission electron microscopy
author_id_str_mv 6ae369618efc7424d9774377536ea519
author_id_fullname_str_mv 6ae369618efc7424d9774377536ea519_***_Richard Palmer
author Richard Palmer
author2 Nan Jian
Miriam Dowle
Richard D Horniblow
Chris Tselepis
Richard Palmer
format Journal article
container_title Nanotechnology
container_volume 27
container_issue 46
container_start_page 46LT02
publishDate 2016
institution Swansea University
issn 0957-4484
1361-6528
doi_str_mv 10.1088/0957-4484/27/46/46lt02
publisher IOP Publishing
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Mechanical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Mechanical Engineering
document_store_str 1
active_str 0
description As the major iron storage protein, ferritin stores and releases iron for maintaining the balance of iron in fauna, flora, and bacteria. We present an investigation of the morphology and iron loading of ferritin (from equine spleen) using aberration-corrected high angle annular dark field scanning transmission electron microscopy. Atom counting method, with size selected Au clusters as mass standards, was employed to determine the number of iron atoms in the nanoparticle core of each ferritin protein. Quantitative analysis shows that the nuclearity of iron atoms in the mineral core varies from a few hundred iron atoms to around 5000 atoms. Moreover, a relationship between the iron loading and iron core morphology is established, in which mineral core nucleates from a single nanoparticle, then grows along the protein shell before finally forming either a solid or hollow core structure.
published_date 2016-11-18T04:00:03Z
_version_ 1763753070248853504
score 11.013148

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