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Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation

Daniel White, Paul Rooks, Susan Kimmance, Karen Tait, Mark Jones, Glen Tarran, Charlotte Cook, Carole Llewellyn

Metabolites, Volume: 9, Issue: 3, Start page: 39

Swansea University Author: Carole Llewellyn

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DOI (Published version): 10.3390/metabo9030039

Abstract

We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (Liquid Chromatography-Tandem Mass Spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultu...

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Published in: Metabolites
ISSN: 2218-1989
Published: Basel, Switzerland MDPI 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa49106
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spelling 2019-07-09T12:17:40.6280304 v2 49106 2019-03-04 Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation bcd94bda79ebf4c2c82d82dfb027a140 Carole Llewellyn Carole Llewellyn true false 2019-03-04 FGSEN We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (Liquid Chromatography-Tandem Mass Spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultures with a significant reduction in monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) class concentrations for nutrient-deplete (LN) cultures. Moreover, reductions in the abundance of MGDG relative to total polar lipids versus an increase in the relative abundance of digalactosyldiacylglycerol (DGDG) were recorded in LN cultures. In HN cultures, polar lipid species composition remained relatively constant throughout culture with high degrees of unsaturation associated with acyl moieties. Conversely, in LN cultures lipid species composition shifted towards greater saturation of acyl moieties. Multivariate analyses revealed that changes in the abundance of a number of species contributed to the dissimilarity between LN and HN cultures but with dominant effects from certain species e.g. reduction in MGDG 34:7 (18:3/16:4). Results demonstrate that Chlorella sp. significantly alters its polar lipidome in response to nutrient limitation and this is discussed in terms of physiological significance and polar lipids production for applied microalgal production systems. Journal Article Metabolites 9 3 39 MDPI Basel, Switzerland 2218-1989 polar lipids; Chlorella sp., LC-MS; nutrient limitation 28 2 2019 2019-02-28 10.3390/metabo9030039 https://www.mdpi.com/ This research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC),UK, grant number BB/K020617/3. ‘Using flow cytometry and genomics to characterise and optimisemicroalgal-bacterial consortia cultivated on wastewater to produce biomass for Biofuel’ COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2019-07-09T12:17:40.6280304 2019-03-04T12:25:56.7559854 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Daniel White 1 Paul Rooks 2 Susan Kimmance 3 Karen Tait 4 Mark Jones 5 Glen Tarran 6 Charlotte Cook 7 Carole Llewellyn 8 0049106-20032019134226.pdf 49106.pdf 2019-03-20T13:42:26.4330000 Output 1411171 application/pdf Version of Record true 2019-03-20T00:00:00.0000000 This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license. true eng
title Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
spellingShingle Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
Carole Llewellyn
title_short Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
title_full Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
title_fullStr Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
title_full_unstemmed Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
title_sort Modulation of Polar Lipid Profiles in Chlorella sp. in Response to Nutrient Limitation
author_id_str_mv bcd94bda79ebf4c2c82d82dfb027a140
author_id_fullname_str_mv bcd94bda79ebf4c2c82d82dfb027a140_***_Carole Llewellyn
author Carole Llewellyn
author2 Daniel White
Paul Rooks
Susan Kimmance
Karen Tait
Mark Jones
Glen Tarran
Charlotte Cook
Carole Llewellyn
format Journal article
container_title Metabolites
container_volume 9
container_issue 3
container_start_page 39
publishDate 2019
institution Swansea University
issn 2218-1989
doi_str_mv 10.3390/metabo9030039
publisher MDPI
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
url https://www.mdpi.com/
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description We evaluate the effects of nutrient limitation on cellular composition of polar lipid classes/species in Chlorella sp. using modern polar lipidomic profiling methods (Liquid Chromatography-Tandem Mass Spectrometry; LC-MS/MS). Total polar lipid concentration was highest in nutrient-replete (HN) cultures with a significant reduction in monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) class concentrations for nutrient-deplete (LN) cultures. Moreover, reductions in the abundance of MGDG relative to total polar lipids versus an increase in the relative abundance of digalactosyldiacylglycerol (DGDG) were recorded in LN cultures. In HN cultures, polar lipid species composition remained relatively constant throughout culture with high degrees of unsaturation associated with acyl moieties. Conversely, in LN cultures lipid species composition shifted towards greater saturation of acyl moieties. Multivariate analyses revealed that changes in the abundance of a number of species contributed to the dissimilarity between LN and HN cultures but with dominant effects from certain species e.g. reduction in MGDG 34:7 (18:3/16:4). Results demonstrate that Chlorella sp. significantly alters its polar lipidome in response to nutrient limitation and this is discussed in terms of physiological significance and polar lipids production for applied microalgal production systems.
published_date 2019-02-28T03:59:53Z
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