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Early Detection and Establishment Assessment of Aquatic Invasive Species / Chloe V. Robinson

DOI (Published version): 10.23889/Suthesis.47968

Abstract

Aquatic invasive species are drivers of ecological change through directly competing with native counterparts, causing alterations in community structure and acting as vectors for the introduction of novel pathogens. A combination of human-mediated introductions and accidental releases from aquacult...

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Published: 2018
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa47968
first_indexed 2018-12-13T20:02:08Z
last_indexed 2019-10-21T16:52:44Z
id cronfa47968
recordtype RisThesis
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spelling 2018-12-14T13:56:42.3344919 v2 47968 2018-12-13 Early Detection and Establishment Assessment of Aquatic Invasive Species 2018-12-13 Aquatic invasive species are drivers of ecological change through directly competing with native counterparts, causing alterations in community structure and acting as vectors for the introduction of novel pathogens. A combination of human-mediated introductions and accidental releases from aquaculture facilities has enabled highly invasive species, including the American signal crayfish (Pacifastacus leniusculus), Chinese mitten crab (Eriocheir sinensis) and topmouth gudgeon (Pseudorasbora parva) to become established in Great Britain. I assessed the factors which could have facilitated their establishment success and dispersal, including genetic diversity. Novel tools such as environmental DNA and citizen science have been proven effective for detecting and monitoring aquatic invasive species. Yet, the motivation for participation and continued data collection in citizen science initiatives are not clear. I have determined that multiple introductions from different source populations are likely to have contributed to the invasion success of signal crayfish in Great Britain. Secondly, I have developed and employed a quantitative PCR environmental DNA multiplex which has enabled simultaneous detection of non-native pathogens (crayfish plague) alongside native and invasive crayfish species, providing information on the coexistence of native and invasive crayfish in absence of crayfish plague. Application of this assay in water and sediment samples has also highlighted the relative impacts of river barriers on mitten crab and signal crayfish dispersal and demonstrated that similar DNA results can be achieved by utilising both types of samples. I also developed a species-specific DNA assay for topmouth gudgeon which detected its presence despite lack of visual confirmation, emphasising the greater sensitivity of environmental DNA tools. Finally, I designed and launched a citizen science initiative in an attempt to assess distribution and pathogen status of signal crayfish, which highlighted the complexity of ensuring participation for successful invasive species initiatives. E-Thesis Invasive species, eDNA, population genetics, citizen science, early detection 31 12 2018 2018-12-31 10.23889/Suthesis.47968 COLLEGE NANME COLLEGE CODE Swansea University Doctoral Ph.D National Research Network for Low-Carbon, Energy and Environment (NRN-LCEE), Welsh Government and Higher Education Funding Council for Wales (hefcw) 2018-12-14T13:56:42.3344919 2018-12-13T16:25:07.1991276 Faculty of Science and Engineering School of Biosciences, Geography and Physics - Biosciences Chloe V. Robinson 1 0047968-14122018134959.pdf 2018_Robinson_631487_Redacted.pdf 2018-12-14T13:49:59.6930000 Output 6439800 application/pdf Redacted version - open access true 2020-01-01T00:00:00.0000000 true
title Early Detection and Establishment Assessment of Aquatic Invasive Species
spellingShingle Early Detection and Establishment Assessment of Aquatic Invasive Species
,
title_short Early Detection and Establishment Assessment of Aquatic Invasive Species
title_full Early Detection and Establishment Assessment of Aquatic Invasive Species
title_fullStr Early Detection and Establishment Assessment of Aquatic Invasive Species
title_full_unstemmed Early Detection and Establishment Assessment of Aquatic Invasive Species
title_sort Early Detection and Establishment Assessment of Aquatic Invasive Species
author ,
author2 Chloe V. Robinson
format E-Thesis
publishDate 2018
institution Swansea University
doi_str_mv 10.23889/Suthesis.47968
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Biosciences, Geography and Physics - Biosciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Biosciences, Geography and Physics - Biosciences
document_store_str 1
active_str 0
description Aquatic invasive species are drivers of ecological change through directly competing with native counterparts, causing alterations in community structure and acting as vectors for the introduction of novel pathogens. A combination of human-mediated introductions and accidental releases from aquaculture facilities has enabled highly invasive species, including the American signal crayfish (Pacifastacus leniusculus), Chinese mitten crab (Eriocheir sinensis) and topmouth gudgeon (Pseudorasbora parva) to become established in Great Britain. I assessed the factors which could have facilitated their establishment success and dispersal, including genetic diversity. Novel tools such as environmental DNA and citizen science have been proven effective for detecting and monitoring aquatic invasive species. Yet, the motivation for participation and continued data collection in citizen science initiatives are not clear. I have determined that multiple introductions from different source populations are likely to have contributed to the invasion success of signal crayfish in Great Britain. Secondly, I have developed and employed a quantitative PCR environmental DNA multiplex which has enabled simultaneous detection of non-native pathogens (crayfish plague) alongside native and invasive crayfish species, providing information on the coexistence of native and invasive crayfish in absence of crayfish plague. Application of this assay in water and sediment samples has also highlighted the relative impacts of river barriers on mitten crab and signal crayfish dispersal and demonstrated that similar DNA results can be achieved by utilising both types of samples. I also developed a species-specific DNA assay for topmouth gudgeon which detected its presence despite lack of visual confirmation, emphasising the greater sensitivity of environmental DNA tools. Finally, I designed and launched a citizen science initiative in an attempt to assess distribution and pathogen status of signal crayfish, which highlighted the complexity of ensuring participation for successful invasive species initiatives.
published_date 2018-12-31T07:28:10Z
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score 11.057624

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