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A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics

Cerys A. Jenkins, Rhys A. Jenkins, Meleri M. Pryse, Kathryn A. Welsby, Maki Jitsumura, Cathy Thornton Orcid Logo, Peter Dunstan Orcid Logo, Dean A. Harris

The Analyst, Volume: 143, Issue: 24, Pages: 6014 - 6024

Swansea University Authors: Cathy Thornton Orcid Logo, Peter Dunstan Orcid Logo

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DOI (Published version): 10.1039/c8an01323c

Abstract

Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous st...

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Published in: The Analyst
ISSN: 0003-2654 1364-5528
Published: 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa45504
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first_indexed 2018-11-08T14:21:59Z
last_indexed 2019-09-02T20:36:00Z
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spelling 2019-09-02T17:22:54.0944192 v2 45504 2018-11-08 A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false eada15d4d33fcb3dfddcff43f1323bd6 0000-0002-4337-4307 Peter Dunstan Peter Dunstan true false 2018-11-08 BMS Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous studies using vibrational spectroscopy with the specific application of diagnosing colorectal diseases such as cancer have mainly focused on in-vivo or in-vitro studies of tissue specimens using microscopy or probe based techniques. There have been few studies of vibrational spectroscopic techniques based on the analysis of blood serum for the advancement of colorectal cancer diagnostics. With growing interest in the field of liquid biopsies, this study presents the development of a high-throughput (HT) serum Raman spectroscopy platform and methodology and compares dry and liquid data acquisition of serum samples. This work considers factors contributing to translatability of the methodologies such as HT design, inter-user variability and sample handling effects on diagnostic capability. The HT Raman methods were tested on a pilot dataset of serum from 30 cancer patients and 30 matched control patients using statistical analysis via cross-validated PLS-DA with a maximum achieved a sensitivity of 83% and specificity of 83% for detecting colorectal cancer. Journal Article The Analyst 143 24 6014 6024 0003-2654 1364-5528 cancer; Raman spectroscopy 1 11 2018 2018-11-01 10.1039/c8an01323c COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-09-02T17:22:54.0944192 2018-11-08T11:09:55.1475028 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Cerys A. Jenkins 1 Rhys A. Jenkins 2 Meleri M. Pryse 3 Kathryn A. Welsby 4 Maki Jitsumura 5 Cathy Thornton 0000-0002-5153-573X 6 Peter Dunstan 0000-0002-4337-4307 7 Dean A. Harris 8 0045504-11122018084454.pdf 45504.pdf 2018-12-11T08:44:54.4400000 Output 25361189 application/pdf Accepted Manuscript true 2019-11-01T00:00:00.0000000 true eng
title A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
spellingShingle A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
Cathy Thornton
Peter Dunstan
title_short A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
title_full A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
title_fullStr A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
title_full_unstemmed A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
title_sort A high-throughput serum Raman spectroscopy platform and methodology for colorectal cancer diagnostics
author_id_str_mv c71a7a4be7361094d046d312202bce0c
eada15d4d33fcb3dfddcff43f1323bd6
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
eada15d4d33fcb3dfddcff43f1323bd6_***_Peter Dunstan
author Cathy Thornton
Peter Dunstan
author2 Cerys A. Jenkins
Rhys A. Jenkins
Meleri M. Pryse
Kathryn A. Welsby
Maki Jitsumura
Cathy Thornton
Peter Dunstan
Dean A. Harris
format Journal article
container_title The Analyst
container_volume 143
container_issue 24
container_start_page 6014
publishDate 2018
institution Swansea University
issn 0003-2654
1364-5528
doi_str_mv 10.1039/c8an01323c
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Vibrational spectroscopic techniques such as Raman spectroscopy and Fourier transform infrared spectroscopy (FTIR) have huge potential for the analysis of biological specimens. The techniques allow the user to gain label-free, non-destructive biochemical information about a given sample. Previous studies using vibrational spectroscopy with the specific application of diagnosing colorectal diseases such as cancer have mainly focused on in-vivo or in-vitro studies of tissue specimens using microscopy or probe based techniques. There have been few studies of vibrational spectroscopic techniques based on the analysis of blood serum for the advancement of colorectal cancer diagnostics. With growing interest in the field of liquid biopsies, this study presents the development of a high-throughput (HT) serum Raman spectroscopy platform and methodology and compares dry and liquid data acquisition of serum samples. This work considers factors contributing to translatability of the methodologies such as HT design, inter-user variability and sample handling effects on diagnostic capability. The HT Raman methods were tested on a pilot dataset of serum from 30 cancer patients and 30 matched control patients using statistical analysis via cross-validated PLS-DA with a maximum achieved a sensitivity of 83% and specificity of 83% for detecting colorectal cancer.
published_date 2018-11-01T03:57:18Z
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score 11.013148

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