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Microneedle-based biosensor for minimally-invasive lactate detection

Paolo Bollella, Sanjiv Sharma Orcid Logo, Anthony Edward George Cass, Riccarda Antiochia

Biosensors and Bioelectronics, Volume: 123, Pages: 152 - 159

Swansea University Author: Sanjiv Sharma Orcid Logo

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DOI (Published version): 10.1016/j.bios.2018.08.010

Abstract

Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). To further demonstrate the capability of microneedle arrays as second generation biosensors we have functionalized gold microneedles with nanoca...

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Published in: Biosensors and Bioelectronics
ISSN: 09565663
Published: 2019
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URI: https://cronfa.swan.ac.uk/Record/cronfa43849
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first_indexed 2018-09-13T18:59:12Z
last_indexed 2020-07-01T18:59:07Z
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spelling 2020-07-01T17:15:30.1418610 v2 43849 2018-09-13 Microneedle-based biosensor for minimally-invasive lactate detection b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2018-09-13 MEDE Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). To further demonstrate the capability of microneedle arrays as second generation biosensors we have functionalized gold microneedles with nanocarbons at which mediated electron transfer of lactate oxidase takes place. In particular the gold surface of the microneedles electrode has been modified in 3 subsequent steps: i) electrodeposition of Au-multiwalled carbon nanotubes (MWCNTs); ii) electropolymerization of the mediator, methylene blue (MB); iii) immobilization of the enzyme lactate oxidase (LOX) by drop-casting procedure. The resulting microneedle-based LOX biosensor displays an interference-free lactate detection without compromising its sensitivity, stability, selectivity and response time. The performance of the microneedle array, second generation biosensor for lactate detection was assessed in artificial interstitial fluid and in human serum, both spiked with lactate. The results reveal that the new mi- croneedles lactate sensor holds interesting promise for the development of a real-time monitoring device to be used in sport medicine and clinical care. Journal Article Biosensors and Bioelectronics 123 152 159 09565663 Microneedles, Lactate, Minimally invasive sensors, Continuous monitoring, Interstitial fluid 31 12 2019 2019-12-31 10.1016/j.bios.2018.08.010 COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-07-01T17:15:30.1418610 2018-09-13T13:06:11.8822254 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Paolo Bollella 1 Sanjiv Sharma 0000-0003-3828-737X 2 Anthony Edward George Cass 3 Riccarda Antiochia 4 0043849-10102018092958.pdf bollella2018.pdf 2018-10-10T09:29:58.8070000 Output 3995229 application/pdf Accepted Manuscript true 2019-08-10T00:00:00.0000000 true eng
title Microneedle-based biosensor for minimally-invasive lactate detection
spellingShingle Microneedle-based biosensor for minimally-invasive lactate detection
Sanjiv Sharma
title_short Microneedle-based biosensor for minimally-invasive lactate detection
title_full Microneedle-based biosensor for minimally-invasive lactate detection
title_fullStr Microneedle-based biosensor for minimally-invasive lactate detection
title_full_unstemmed Microneedle-based biosensor for minimally-invasive lactate detection
title_sort Microneedle-based biosensor for minimally-invasive lactate detection
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Sanjiv Sharma
author2 Paolo Bollella
Sanjiv Sharma
Anthony Edward George Cass
Riccarda Antiochia
format Journal article
container_title Biosensors and Bioelectronics
container_volume 123
container_start_page 152
publishDate 2019
institution Swansea University
issn 09565663
doi_str_mv 10.1016/j.bios.2018.08.010
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
document_store_str 1
active_str 0
description Here we report the first mediated microneedles-based biosensor for minimally invasive continuous sensing of lactate in the dermal interstitial fluid (ISF). To further demonstrate the capability of microneedle arrays as second generation biosensors we have functionalized gold microneedles with nanocarbons at which mediated electron transfer of lactate oxidase takes place. In particular the gold surface of the microneedles electrode has been modified in 3 subsequent steps: i) electrodeposition of Au-multiwalled carbon nanotubes (MWCNTs); ii) electropolymerization of the mediator, methylene blue (MB); iii) immobilization of the enzyme lactate oxidase (LOX) by drop-casting procedure. The resulting microneedle-based LOX biosensor displays an interference-free lactate detection without compromising its sensitivity, stability, selectivity and response time. The performance of the microneedle array, second generation biosensor for lactate detection was assessed in artificial interstitial fluid and in human serum, both spiked with lactate. The results reveal that the new mi- croneedles lactate sensor holds interesting promise for the development of a real-time monitoring device to be used in sport medicine and clinical care.
published_date 2019-12-31T03:55:13Z
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score 11.013686

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