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Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines

Kuo-Ching Mei, Artur Ghazaryan, Er Zhen Teoh, Huw Summers Orcid Logo, Yueting Li, Belén Ballesteros, Justyna Piasecka, Adam Walters, Robert C. Hider, Volker Mailänder, Khuloud T. Al-Jamal

Advanced Materials, Volume: 30, Issue: 40, Start page: 1802732

Swansea University Authors: Huw Summers Orcid Logo, Justyna Piasecka

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DOI (Published version): 10.1002/adma.201802732

Abstract

Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticl...

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Published in: Advanced Materials
ISSN: 0935-9648
Published: Wiley 2018
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URI: https://cronfa.swan.ac.uk/Record/cronfa43705
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Due to the size, curvature, and specific surface area (SSA) 3&#x2010;factor interactions inherited in the traditional 3D nanoparticle, HC&#x2010;dependent bio&#x2013;nano interactions are often poorly probed and interpreted. Here, the first HC&#x2010;by&#x2010;design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label&#x2010;free liquid chromatography&#x2013;mass spectrometry (LC&#x2010;MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size&#x2013;curvature&#x2013;SSA multifactor interactions and can serve as a reliable screening platform to uncover HC&#x2010;dependent bio&#x2013;nano interactions to enable the next&#x2010;generation quality&#x2010;by&#x2010;design (QbD) nanomedicines for better clinical translation.</abstract><type>Journal Article</type><journal>Advanced Materials</journal><volume>30</volume><journalNumber>40</journalNumber><paginationStart>1802732</paginationStart><publisher>Wiley</publisher><issnPrint>0935-9648</issnPrint><keywords/><publishedDay>1</publishedDay><publishedMonth>10</publishedMonth><publishedYear>2018</publishedYear><publishedDate>2018-10-01</publishedDate><doi>10.1002/adma.201802732</doi><url/><notes>This inter-disciplinary paper addresses the critical issue in nano-biotechnology of the protein corona that surrounds nanoparticles. It presents the first engineered approach to control the corona using graphene materials. The importance of the Swansea contribution was in the provision of detailed, quantitative analysis of nanoparticle decoration of cells using imaging cytometry. This was crucial in allowing interpretation of dimensionless PCA scores into meaningful biological function using correlation studies, based on the imaging technology.</notes><college>COLLEGE NANME</college><department>Biomedical Engineering</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>MEDE</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-07-25T18:36:36.3608577</lastEdited><Created>2018-09-06T09:59:25.8635496</Created><authors><author><firstname>Kuo-Ching</firstname><surname>Mei</surname><order>1</order></author><author><firstname>Artur</firstname><surname>Ghazaryan</surname><order>2</order></author><author><firstname>Er Zhen</firstname><surname>Teoh</surname><order>3</order></author><author><firstname>Huw</firstname><surname>Summers</surname><orcid>0000-0002-0898-5612</orcid><order>4</order></author><author><firstname>Yueting</firstname><surname>Li</surname><order>5</order></author><author><firstname>Bel&#xE9;n</firstname><surname>Ballesteros</surname><order>6</order></author><author><firstname>Justyna</firstname><surname>Piasecka</surname><orcid/><order>7</order></author><author><firstname>Adam</firstname><surname>Walters</surname><order>8</order></author><author><firstname>Robert C.</firstname><surname>Hider</surname><order>9</order></author><author><firstname>Volker</firstname><surname>Mail&#xE4;nder</surname><order>10</order></author><author><firstname>Khuloud T.</firstname><surname>Al-Jamal</surname><order>11</order></author></authors><documents><document><filename>0043705-06092018100506.pdf</filename><originalFilename>mei2018(2).pdf</originalFilename><uploaded>2018-09-06T10:05:06.3230000</uploaded><type>Output</type><contentLength>2554106</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language><licence>http://creativecommons.org/licenses/by/4.0/</licence></document></documents><OutputDurs/></rfc1807>
spelling 2020-07-25T18:36:36.3608577 v2 43705 2018-09-06 Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines a61c15e220837ebfa52648c143769427 0000-0002-0898-5612 Huw Summers Huw Summers true false 929d37d0bcff9a9c2d7dd74fe120b22e Justyna Piasecka Justyna Piasecka true false 2018-09-06 MEDE Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticle, HC‐dependent bio–nano interactions are often poorly probed and interpreted. Here, the first HC‐by‐design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label‐free liquid chromatography–mass spectrometry (LC‐MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size–curvature–SSA multifactor interactions and can serve as a reliable screening platform to uncover HC‐dependent bio–nano interactions to enable the next‐generation quality‐by‐design (QbD) nanomedicines for better clinical translation. Journal Article Advanced Materials 30 40 1802732 Wiley 0935-9648 1 10 2018 2018-10-01 10.1002/adma.201802732 This inter-disciplinary paper addresses the critical issue in nano-biotechnology of the protein corona that surrounds nanoparticles. It presents the first engineered approach to control the corona using graphene materials. The importance of the Swansea contribution was in the provision of detailed, quantitative analysis of nanoparticle decoration of cells using imaging cytometry. This was crucial in allowing interpretation of dimensionless PCA scores into meaningful biological function using correlation studies, based on the imaging technology. COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2020-07-25T18:36:36.3608577 2018-09-06T09:59:25.8635496 Kuo-Ching Mei 1 Artur Ghazaryan 2 Er Zhen Teoh 3 Huw Summers 0000-0002-0898-5612 4 Yueting Li 5 Belén Ballesteros 6 Justyna Piasecka 7 Adam Walters 8 Robert C. Hider 9 Volker Mailänder 10 Khuloud T. Al-Jamal 11 0043705-06092018100506.pdf mei2018(2).pdf 2018-09-06T10:05:06.3230000 Output 2554106 application/pdf Version of Record true Released under the terms of a Creative Commons Attribution License (CC-BY). true eng http://creativecommons.org/licenses/by/4.0/
title Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
spellingShingle Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
Huw Summers
Justyna Piasecka
title_short Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
title_full Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
title_fullStr Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
title_full_unstemmed Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
title_sort Protein-Corona-by-Design in 2D: A Reliable Platform to Decode Bio-Nano Interactions for the Next-Generation Quality-by-Design Nanomedicines
author_id_str_mv a61c15e220837ebfa52648c143769427
929d37d0bcff9a9c2d7dd74fe120b22e
author_id_fullname_str_mv a61c15e220837ebfa52648c143769427_***_Huw Summers
929d37d0bcff9a9c2d7dd74fe120b22e_***_Justyna Piasecka
author Huw Summers
Justyna Piasecka
author2 Kuo-Ching Mei
Artur Ghazaryan
Er Zhen Teoh
Huw Summers
Yueting Li
Belén Ballesteros
Justyna Piasecka
Adam Walters
Robert C. Hider
Volker Mailänder
Khuloud T. Al-Jamal
format Journal article
container_title Advanced Materials
container_volume 30
container_issue 40
container_start_page 1802732
publishDate 2018
institution Swansea University
issn 0935-9648
doi_str_mv 10.1002/adma.201802732
publisher Wiley
document_store_str 1
active_str 0
description Hard corona (HC) protein, i.e., the environmental proteins of the biological medium that are bound to a nanosurface, is known to affect the biological fate of a nanomedicine. Due to the size, curvature, and specific surface area (SSA) 3‐factor interactions inherited in the traditional 3D nanoparticle, HC‐dependent bio–nano interactions are often poorly probed and interpreted. Here, the first HC‐by‐design case study in 2D is demonstrated that sequentially and linearly changes the HC quantity using functionalized graphene oxide (GO) nanosheets. The HC quantity and HC quality are analyzed using NanoDrop and label‐free liquid chromatography–mass spectrometry (LC‐MS) followed by principal component analysis (PCA). Cellular responses (uptake and cytotoxicity in J774 cell model) are compared using imaging cytometry and the modified lactate dehydrogenase assays, respectively. Cellular uptake linearly and solely correlates with HC quantity (R2 = 0.99634). The nanotoxicity, analyzed by retrospective design of experiment (DoE), is found to be dependent on the nanomaterial uptake (primary), HC composition (secondary), and nanomaterial exposure dose (tertiary). This unique 2D design eliminates the size–curvature–SSA multifactor interactions and can serve as a reliable screening platform to uncover HC‐dependent bio–nano interactions to enable the next‐generation quality‐by‐design (QbD) nanomedicines for better clinical translation.
published_date 2018-10-01T03:55:01Z
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score 11.013371

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