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Characterisation of osteopontin and CD44 in endometrium of infertile women. / Natalie Victoria De Mello

Swansea University Author: Natalie Victoria De Mello

Abstract

Cell adhesion proteins osteopontin, CD44 and integrin alphaVbeta3 interact to form an adhesion complex between the embryo and endometrial surface forming an attachment that can lead to implantation. Whilst receptivity has been investigated extensively, the expression of this adhesion complex has yet...

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Published: 2013
Institution: Swansea University
Degree level: Doctoral
Degree name: Ph.D
URI: https://cronfa.swan.ac.uk/Record/cronfa42761
first_indexed 2018-08-02T18:55:29Z
last_indexed 2019-10-21T16:48:25Z
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recordtype RisThesis
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spelling 2018-08-29T14:35:47.0991309 v2 42761 2018-08-02 Characterisation of osteopontin and CD44 in endometrium of infertile women. e9fb426624981b90589c4cf00601d77f NULL Natalie Victoria De Mello Natalie Victoria De Mello true true 2018-08-02 Cell adhesion proteins osteopontin, CD44 and integrin alphaVbeta3 interact to form an adhesion complex between the embryo and endometrial surface forming an attachment that can lead to implantation. Whilst receptivity has been investigated extensively, the expression of this adhesion complex has yet to be studied simultaneously in the endometrium. This thesis establishes the expression of the adhesion complex in fertile and infertile endometrium. In addition the regulation of the adhesion complex components by distinct signalling pathways and the key regulators estrogen receptor, nuclear factor kappa B and signal transducer and activator of transcription 1 have been investigated in endometrial cell lines. Objectives: To establish the expression profile of adhesion complex components in samples obtained from fertile and infertile women. To model in vitro hormonal regulation of adhesion complex components to mimic estrogen and progesterone stimulus in the menstrual cycle. To determine if adverse environments common to poly cystic ovarian syndrome and endometriosis affect uterine expression of the adhesion complex via high glucose and pro-inflammatory cytokines. To investigate the direct regulation of Osteopontin and CD44 by estrogen and cytokine signalling through estrogen receptor ?, nuclear factor kappa B and signal transducer and activator of transcription 1. Methodology: Investigation of human biopsies and cell line models by immunohistochemistry, quantitative polymerase chain reaction, chromatin immunoprecipitation and enzyme linked immunosorbent assay. Conclusions: Adverse uterine environments including high glucose and pro-inflammatory cytokines may regulate the expression of the adhesion complex, and contribute to a lack of endometrial receptivity in endometriosis and poly cystic ovarian patients. CD44, ITGAV and ITGB3 levels may be used as markers for loss of receptivity in unexplained infertility. E-Thesis Medicine.;Obstetrics. 31 12 2013 2013-12-31 COLLEGE NANME Swansea University Medical School COLLEGE CODE Swansea University Doctoral Ph.D 2018-08-29T14:35:47.0991309 2018-08-02T16:24:30.3985985 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Natalie Victoria De Mello NULL 1 0042761-02082018162519.pdf 10807530.pdf 2018-08-02T16:25:19.8970000 Output 24951912 application/pdf E-Thesis true 2018-08-02T16:25:19.8970000 false
title Characterisation of osteopontin and CD44 in endometrium of infertile women.
spellingShingle Characterisation of osteopontin and CD44 in endometrium of infertile women.
Natalie Victoria De Mello
title_short Characterisation of osteopontin and CD44 in endometrium of infertile women.
title_full Characterisation of osteopontin and CD44 in endometrium of infertile women.
title_fullStr Characterisation of osteopontin and CD44 in endometrium of infertile women.
title_full_unstemmed Characterisation of osteopontin and CD44 in endometrium of infertile women.
title_sort Characterisation of osteopontin and CD44 in endometrium of infertile women.
author_id_str_mv e9fb426624981b90589c4cf00601d77f
author_id_fullname_str_mv e9fb426624981b90589c4cf00601d77f_***_Natalie Victoria De Mello
author Natalie Victoria De Mello
author2 Natalie Victoria De Mello
format E-Thesis
publishDate 2013
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Cell adhesion proteins osteopontin, CD44 and integrin alphaVbeta3 interact to form an adhesion complex between the embryo and endometrial surface forming an attachment that can lead to implantation. Whilst receptivity has been investigated extensively, the expression of this adhesion complex has yet to be studied simultaneously in the endometrium. This thesis establishes the expression of the adhesion complex in fertile and infertile endometrium. In addition the regulation of the adhesion complex components by distinct signalling pathways and the key regulators estrogen receptor, nuclear factor kappa B and signal transducer and activator of transcription 1 have been investigated in endometrial cell lines. Objectives: To establish the expression profile of adhesion complex components in samples obtained from fertile and infertile women. To model in vitro hormonal regulation of adhesion complex components to mimic estrogen and progesterone stimulus in the menstrual cycle. To determine if adverse environments common to poly cystic ovarian syndrome and endometriosis affect uterine expression of the adhesion complex via high glucose and pro-inflammatory cytokines. To investigate the direct regulation of Osteopontin and CD44 by estrogen and cytokine signalling through estrogen receptor ?, nuclear factor kappa B and signal transducer and activator of transcription 1. Methodology: Investigation of human biopsies and cell line models by immunohistochemistry, quantitative polymerase chain reaction, chromatin immunoprecipitation and enzyme linked immunosorbent assay. Conclusions: Adverse uterine environments including high glucose and pro-inflammatory cytokines may regulate the expression of the adhesion complex, and contribute to a lack of endometrial receptivity in endometriosis and poly cystic ovarian patients. CD44, ITGAV and ITGB3 levels may be used as markers for loss of receptivity in unexplained infertility.
published_date 2013-12-31T13:36:11Z
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score 11.139166

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