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Investigations of androgenic steroids by liquid chromatography / mass spectrometry. / Jinglei Yu
Swansea University Author: Jinglei Yu
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Androgen steroids are an important group in the hormone family. As they play significant roles in the human body, identification and determination of these compounds have been of great interest in many fields. In this thesis, systematic studies of the compounds in the androgen group by liquid chroma...
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Androgen steroids are an important group in the hormone family. As they play significant roles in the human body, identification and determination of these compounds have been of great interest in many fields. In this thesis, systematic studies of the compounds in the androgen group by liquid chromatography / mass spectrometry have been undertaken. The first chapter gives a brief introduction to the structure, function and classification of the steroid family, and is followed by a review of different analytical techniques applied in this family. The theory behind the chromatographic and mass spectrometric techniques used has also been introduced. Structural information about the androgenic steroids and their conjugates has been obtained in chapters 2 and 3 respectively by both ESI and APCI mass spectrometry. The effect of different solvent systems was studied in detail in both the positive and negative ionization modes and the collision induced dissociation of these compounds was also investigated by tandem mass spectrometry. In chapter 4, LC/MS methods have been optimized in terms of columns, solvent systems, flow rate and mass spectrometer parameters to quantify some of the androgens and their conjugates respectively. The linearity, precision and limits of detection were estimated for these compounds. A derivatization method was developed to enhance the sensitivities for detection of the androgens and their conjugates by LC/MS in the final chapter. A TMPP reagent was used as a derivatizing reagent, and the spectra of these TMPP-derivatives of androgens were studied by ESI mass spectrometry. A LC/MS method was also set up with the aim of quantitation of five TMPP-derivatives of androgens.
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