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The development of nanoscale techniques for the study of electrodes associated with biochemical processes. / Daniel James Gordon

Swansea University Author: Daniel James Gordon

Abstract

Bioelectrochemistry is a growing area with a great deal to offer technology, particularly in the fields of power generation, biosensors and medicine. The interfacial processes between electrodes and biological materials are central to understanding and exploitation of these processes. The aim of thi...

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Published: 2011
Institution: Swansea University
Degree level: Master of Philosophy
Degree name: M.Phil
URI: https://cronfa.swan.ac.uk/Record/cronfa42535
first_indexed 2018-08-02T18:54:56Z
last_indexed 2019-10-21T16:48:00Z
id cronfa42535
recordtype RisThesis
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spelling 2018-08-16T14:39:02.9105634 v2 42535 2018-08-02 The development of nanoscale techniques for the study of electrodes associated with biochemical processes. 8fddf18da2257861f5a561b85abc9ff9 NULL Daniel James Gordon Daniel James Gordon true true 2018-08-02 Bioelectrochemistry is a growing area with a great deal to offer technology, particularly in the fields of power generation, biosensors and medicine. The interfacial processes between electrodes and biological materials are central to understanding and exploitation of these processes. The aim of this project was to investigate these processes by studying the interactions at the electrode surfaces. A number of techniques were employed to investigate the types of electrodes and the chemical reactivity with particular reference to biological materials Initial studies of electrode characteristics were carried out through cyclic voltammetry studies of methyl viologen and oxygen. In the study different electrodes and different surface finishes are investigated. The topology of the electrodes is examined through the use of Atomic Force Microscopy (AFM), giving both images and statistical analysis of the surface roughness. Finally the bioelectrochemistry of proteins on the electrodes are investigated using the studies of laccase, azurin and the fouling of the electrode by bovine serum albumin. Although establishing the basic methods and techniques required to study proteins on surfaces, the study did not give conclusive results as to electrode choice or the nature of the protein electrode interaction. It does however; indicate that a pyrolytic graphite edge plane electrode polished with alumina slurry is likely to be the best electrode for use with proteins. The studies with proteins need to be developed further and recent publications suggest several new methods that could be used. During the work, many obstacles were encountered in the use of proteins, so this study puts forward possible methods of avoiding the same problems in the future. Likewise, new methods of investigation of electrode surfaces are put forward including Conducting-AFM, Electromagnetic Force Microscopy (EFM) and the imaging of double layers at a charged electrode using an AFM in fluid. E-Thesis Biomedical engineering.;Electrical engineering.;Nanotechnology. 31 12 2011 2011-12-31 COLLEGE NANME Engineering COLLEGE CODE Swansea University Master of Philosophy M.Phil 2018-08-16T14:39:02.9105634 2018-08-02T16:24:29.5873977 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Daniel James Gordon NULL 1 0042535-02082018162502.pdf 10805284.pdf 2018-08-02T16:25:02.1600000 Output 16336719 application/pdf E-Thesis true 2018-08-02T16:25:02.1600000 false
title The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
spellingShingle The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
Daniel James Gordon
title_short The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
title_full The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
title_fullStr The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
title_full_unstemmed The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
title_sort The development of nanoscale techniques for the study of electrodes associated with biochemical processes.
author_id_str_mv 8fddf18da2257861f5a561b85abc9ff9
author_id_fullname_str_mv 8fddf18da2257861f5a561b85abc9ff9_***_Daniel James Gordon
author Daniel James Gordon
author2 Daniel James Gordon
format E-Thesis
publishDate 2011
institution Swansea University
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
document_store_str 1
active_str 0
description Bioelectrochemistry is a growing area with a great deal to offer technology, particularly in the fields of power generation, biosensors and medicine. The interfacial processes between electrodes and biological materials are central to understanding and exploitation of these processes. The aim of this project was to investigate these processes by studying the interactions at the electrode surfaces. A number of techniques were employed to investigate the types of electrodes and the chemical reactivity with particular reference to biological materials Initial studies of electrode characteristics were carried out through cyclic voltammetry studies of methyl viologen and oxygen. In the study different electrodes and different surface finishes are investigated. The topology of the electrodes is examined through the use of Atomic Force Microscopy (AFM), giving both images and statistical analysis of the surface roughness. Finally the bioelectrochemistry of proteins on the electrodes are investigated using the studies of laccase, azurin and the fouling of the electrode by bovine serum albumin. Although establishing the basic methods and techniques required to study proteins on surfaces, the study did not give conclusive results as to electrode choice or the nature of the protein electrode interaction. It does however; indicate that a pyrolytic graphite edge plane electrode polished with alumina slurry is likely to be the best electrode for use with proteins. The studies with proteins need to be developed further and recent publications suggest several new methods that could be used. During the work, many obstacles were encountered in the use of proteins, so this study puts forward possible methods of avoiding the same problems in the future. Likewise, new methods of investigation of electrode surfaces are put forward including Conducting-AFM, Electromagnetic Force Microscopy (EFM) and the imaging of double layers at a charged electrode using an AFM in fluid.
published_date 2011-12-31T17:24:21Z
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score 11.059359

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