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Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase

Thomas R. Sewell, Nichola J. Hawkins, Henrik U. Stotz, YongJu Huang, Steven Kelly Orcid Logo, Diane Kelly, Bart Fraaije, Bruce D. L. Fitt

Scientific Reports, Volume: 7, Issue: 1

Swansea University Authors: Steven Kelly Orcid Logo, Diane Kelly

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DOI (Published version): 10.1038/s41598-017-15545-9

Abstract

Lanosterol 14-α demethylase is a key enzyme intermediating the biosynthesis of ergosterol in fungi, and the target of azole fungicides. Studies have suggested that Leptosphaeria maculans and L. biglobosa, the causal agents of phoma stem canker on oilseed rape, differ in their sensitivity to some azo...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa36850
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spelling 2020-06-19T12:58:23.7066523 v2 36850 2017-11-20 Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase b17cebaf09b4d737b9378a3581e3de93 0000-0001-7991-5040 Steven Kelly Steven Kelly true false 5ccf81e5d5beedf32ef8d7c3d7ac6c8c Diane Kelly Diane Kelly true false 2017-11-20 BMS Lanosterol 14-α demethylase is a key enzyme intermediating the biosynthesis of ergosterol in fungi, and the target of azole fungicides. Studies have suggested that Leptosphaeria maculans and L. biglobosa, the causal agents of phoma stem canker on oilseed rape, differ in their sensitivity to some azoles, which could be driving pathogen frequency change in crops. Here we used CYP51 protein modelling and heterologous expression to determine whether there are interspecific differences at the target-site level. Moreover, we provide an example of intrinsic sensitivity differences exhibited by both Leptosphaeria spp. in vitro and in planta. Comparison of homologous protein models identified highly conserved residues, particularly at the azole binding site, and heterologous expression of LmCYP51B and LbCYP51B, with fungicide sensitivity testing of the transformants, suggests that both proteins are similarly sensitive to azole fungicides flusilazole, prothioconazole-desthio and tebuconazole. Fungicide sensitivity testing on isolates shows that they sometimes have a minor difference in sensitivity in vitro and in planta. These results suggest that azole fungicides remain a useful component of integrated phoma stem canker control in the UK due to their effectiveness on both Leptosphaeria spp. Other factors, such as varietal resistance or climate, may be driving observed frequency changes between species. Journal Article Scientific Reports 7 1 2045-2322 Antifungal agents, plant molecular biology 31 12 2017 2017-12-31 10.1038/s41598-017-15545-9 This article is licensed under a Creative Commons Attribution 4.0 International License. COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2020-06-19T12:58:23.7066523 2017-11-20T16:00:44.7618355 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Thomas R. Sewell 1 Nichola J. Hawkins 2 Henrik U. Stotz 3 YongJu Huang 4 Steven Kelly 0000-0001-7991-5040 5 Diane Kelly 6 Bart Fraaije 7 Bruce D. L. Fitt 8 0036850-15122017092829.pdf 36850.pdf 2017-12-15T09:28:29.9930000 Output 1970962 application/pdf Version of Record true 2017-12-15T00:00:00.0000000 This article is licensed under a Creative Commons Attribution 4.0 International License. true eng
title Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
spellingShingle Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
Steven Kelly
Diane Kelly
title_short Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
title_full Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
title_fullStr Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
title_full_unstemmed Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
title_sort Azole sensitivity in Leptosphaeria pathogens of oilseed rape: the role of lanosterol 14α-demethylase
author_id_str_mv b17cebaf09b4d737b9378a3581e3de93
5ccf81e5d5beedf32ef8d7c3d7ac6c8c
author_id_fullname_str_mv b17cebaf09b4d737b9378a3581e3de93_***_Steven Kelly
5ccf81e5d5beedf32ef8d7c3d7ac6c8c_***_Diane Kelly
author Steven Kelly
Diane Kelly
author2 Thomas R. Sewell
Nichola J. Hawkins
Henrik U. Stotz
YongJu Huang
Steven Kelly
Diane Kelly
Bart Fraaije
Bruce D. L. Fitt
format Journal article
container_title Scientific Reports
container_volume 7
container_issue 1
publishDate 2017
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-017-15545-9
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Lanosterol 14-α demethylase is a key enzyme intermediating the biosynthesis of ergosterol in fungi, and the target of azole fungicides. Studies have suggested that Leptosphaeria maculans and L. biglobosa, the causal agents of phoma stem canker on oilseed rape, differ in their sensitivity to some azoles, which could be driving pathogen frequency change in crops. Here we used CYP51 protein modelling and heterologous expression to determine whether there are interspecific differences at the target-site level. Moreover, we provide an example of intrinsic sensitivity differences exhibited by both Leptosphaeria spp. in vitro and in planta. Comparison of homologous protein models identified highly conserved residues, particularly at the azole binding site, and heterologous expression of LmCYP51B and LbCYP51B, with fungicide sensitivity testing of the transformants, suggests that both proteins are similarly sensitive to azole fungicides flusilazole, prothioconazole-desthio and tebuconazole. Fungicide sensitivity testing on isolates shows that they sometimes have a minor difference in sensitivity in vitro and in planta. These results suggest that azole fungicides remain a useful component of integrated phoma stem canker control in the UK due to their effectiveness on both Leptosphaeria spp. Other factors, such as varietal resistance or climate, may be driving observed frequency changes between species.
published_date 2017-12-31T03:46:13Z
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