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Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro

InaLaura Pieper, Gemma Radley, Abigail Christen, Sabrina Ali, Owen Bodger Orcid Logo, Cathy Thornton Orcid Logo

Artificial Organs

Swansea University Authors: Owen Bodger Orcid Logo, Cathy Thornton Orcid Logo

DOI (Published version): 10.1111/aor.13068

Abstract

Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and p...

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Published in: Artificial Organs
Published: 2018
URI: https://cronfa.swan.ac.uk/Record/cronfa36276
first_indexed 2017-10-26T13:34:23Z
last_indexed 2020-06-03T18:49:12Z
id cronfa36276
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spelling 2020-06-03T13:33:00.2485374 v2 36276 2017-10-26 Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro 8096440ab42b60a86e6aba678fe2695a 0000-0002-4022-9964 Owen Bodger Owen Bodger true false c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-10-26 MEDS Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and proteins caused by mechanical stress and foreign materials. Studies of the effects of VADs on leukocytes are limited, yet leukocyte activation and damage including microparticle generation can influence both thrombosis and infection rates. Therefore, the aim was to develop a multicolour flow cytometry assessment of leukocyte microparticles (LMPs) using ovine blood and the CentriMag VAD as a model for shear stress. Ovine blood was pumped for 6 hours in the CentriMag and regular samples analysed for haemolysis, complete blood counts and leukocyte microparticles by flow cytometry during three different pump operating conditions (low flow, standard, high speed). The high speed condition caused significant increases in plasma-free haemoglobin; decreases in total leukocytes, granulocytes, monocytes and platelets; increases in CD45+ LMPs as well as two novel LMP populations: CD11bbright/HLA-DR- and CD11bdull/HLA-DR+, both of which were CD14-/CD21-. CD11bbright/HLA-DR- LMPs appeared to respond to an increase in shear magnitude whereas the CD11bdull/HLA-DR+ LMPs significantly increased in all pumping conditions. We propose that these two populations are released from granulocytes and T cells, respectively, but further research is needed to better characterise these two populations. Journal Article Artificial Organs Flow cytometry, ovine, leukocyte, microparticle, shear stress, ventricular assist, CD11b, HLA-DR, CentriMag 7 3 2018 2018-03-07 10.1111/aor.13068 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2020-06-03T13:33:00.2485374 2017-10-26T11:23:57.3711474 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine InaLaura Pieper 1 Gemma Radley 2 Abigail Christen 3 Sabrina Ali 4 Owen Bodger 0000-0002-4022-9964 5 Cathy Thornton 0000-0002-5153-573X 6 0036276-17052018164204.pdf OvineactivatedMPsintheCMAG_FinalforSUMSv2.pdf 2018-05-17T16:42:04.0970000 Output 1893252 application/pdf Accepted Manuscript true 2019-03-07T00:00:00.0000000 true eng
title Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
spellingShingle Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
Owen Bodger
Cathy Thornton
title_short Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_full Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_fullStr Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_full_unstemmed Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
title_sort Ovine leukocyte microparticles generated by the CentriMag ventricular assist device in vitro
author_id_str_mv 8096440ab42b60a86e6aba678fe2695a
c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv 8096440ab42b60a86e6aba678fe2695a_***_Owen Bodger
c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author Owen Bodger
Cathy Thornton
author2 InaLaura Pieper
Gemma Radley
Abigail Christen
Sabrina Ali
Owen Bodger
Cathy Thornton
format Journal article
container_title Artificial Organs
publishDate 2018
institution Swansea University
doi_str_mv 10.1111/aor.13068
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Ventricular assist devices (VADs) are a life-saving form of mechanical circulatory support in heart failure patients. However, VADs have not yet reached their full potential due to the associated side effects (thrombosis, bleeding, infection) related to the activation and damage of blood cells and proteins caused by mechanical stress and foreign materials. Studies of the effects of VADs on leukocytes are limited, yet leukocyte activation and damage including microparticle generation can influence both thrombosis and infection rates. Therefore, the aim was to develop a multicolour flow cytometry assessment of leukocyte microparticles (LMPs) using ovine blood and the CentriMag VAD as a model for shear stress. Ovine blood was pumped for 6 hours in the CentriMag and regular samples analysed for haemolysis, complete blood counts and leukocyte microparticles by flow cytometry during three different pump operating conditions (low flow, standard, high speed). The high speed condition caused significant increases in plasma-free haemoglobin; decreases in total leukocytes, granulocytes, monocytes and platelets; increases in CD45+ LMPs as well as two novel LMP populations: CD11bbright/HLA-DR- and CD11bdull/HLA-DR+, both of which were CD14-/CD21-. CD11bbright/HLA-DR- LMPs appeared to respond to an increase in shear magnitude whereas the CD11bdull/HLA-DR+ LMPs significantly increased in all pumping conditions. We propose that these two populations are released from granulocytes and T cells, respectively, but further research is needed to better characterise these two populations.
published_date 2018-03-07T04:18:38Z
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score 11.29607

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