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Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications

Jakub Trzebinski, Sanjiv Sharma Orcid Logo, Anna Radomska-Botelho Moniz, Kostis Michelakis, Yangyang Zhang, Anthony E. G. Cass

Lab Chip, Volume: 12, Issue: 2, Pages: 348 - 352

Swansea University Author: Sanjiv Sharma Orcid Logo

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DOI (Published version): 10.1039/C1LC20885C

Abstract

In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling che...

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Published in: Lab Chip
ISSN: 1473-0189
Published: 2012
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URI: https://cronfa.swan.ac.uk/Record/cronfa36258
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first_indexed 2017-10-25T19:11:57Z
last_indexed 2018-02-09T05:28:19Z
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spelling 2017-10-26T09:18:43.3549800 v2 36258 2017-10-25 Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications b6b7506358522f607b171ec9c94757b7 0000-0003-3828-737X Sanjiv Sharma Sanjiv Sharma true false 2017-10-25 MEDE In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling chemistry. An epoxy-polyurethane based membrane was used to extend the linear working range (from 0 to 25mM of substrate) of these biosensors. Both lactate and glucose sensors performed well in the clinically relevant substrate concentration range. Glucose microspikes were further investigated with respect to the effects of substrate transfer by incorporation into a microfluidic system. Data from the microfluidic system revealed that the sensor response is mainly dependent on enzyme kinetics rather than membrane permeability to glucose. The robustness of the sensors was demonstrated by its consistency in performance extending over 48 h. Journal Article Lab Chip 12 2 348 352 1473-0189 30 11 2012 2012-11-30 10.1039/C1LC20885C http://pubs.rsc.org/en/content/articlelanding/2012/lc/c1lc20885c#!divAbstract COLLEGE NANME Biomedical Engineering COLLEGE CODE MEDE Swansea University 2017-10-26T09:18:43.3549800 2017-10-25T14:06:26.6102146 Faculty of Science and Engineering School of Engineering and Applied Sciences - Biomedical Engineering Jakub Trzebinski 1 Sanjiv Sharma 0000-0003-3828-737X 2 Anna Radomska-Botelho Moniz 3 Kostis Michelakis 4 Yangyang Zhang 5 Anthony E. G. Cass 6
title Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
spellingShingle Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
Sanjiv Sharma
title_short Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
title_full Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
title_fullStr Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
title_full_unstemmed Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
title_sort Microfluidic device to investigate factors affecting performance in biosensors designed for transdermal applications
author_id_str_mv b6b7506358522f607b171ec9c94757b7
author_id_fullname_str_mv b6b7506358522f607b171ec9c94757b7_***_Sanjiv Sharma
author Sanjiv Sharma
author2 Jakub Trzebinski
Sanjiv Sharma
Anna Radomska-Botelho Moniz
Kostis Michelakis
Yangyang Zhang
Anthony E. G. Cass
format Journal article
container_title Lab Chip
container_volume 12
container_issue 2
container_start_page 348
publishDate 2012
institution Swansea University
issn 1473-0189
doi_str_mv 10.1039/C1LC20885C
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Biomedical Engineering{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Biomedical Engineering
url http://pubs.rsc.org/en/content/articlelanding/2012/lc/c1lc20885c#!divAbstract
document_store_str 0
active_str 0
description In this work we demonstrate a novel microfluidic based platform to investigate the performance of 3D out-of-plane microspike array based glucose and lactate biosensors. The microspike array was bonded with a glass slide and modified with glucose oxidase or lactate oxidase using covalent coupling chemistry. An epoxy-polyurethane based membrane was used to extend the linear working range (from 0 to 25mM of substrate) of these biosensors. Both lactate and glucose sensors performed well in the clinically relevant substrate concentration range. Glucose microspikes were further investigated with respect to the effects of substrate transfer by incorporation into a microfluidic system. Data from the microfluidic system revealed that the sensor response is mainly dependent on enzyme kinetics rather than membrane permeability to glucose. The robustness of the sensors was demonstrated by its consistency in performance extending over 48 h.
published_date 2012-11-30T03:45:16Z
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score 11.013148

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