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Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)

Richard Metcalfe Orcid Logo, F. Koumanov, J. S. Ruffino, K. A. Stokes, G. D. Holman, D. Thompson, N. B. J. Vollaard

European Journal of Applied Physiology, Volume: 115, Issue: 11, Pages: 2321 - 2334

Swansea University Author: Richard Metcalfe Orcid Logo

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DOI (Published version): 10.1007/s00421-015-3217-6

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PurposeWe have previously shown that 6 weeks of reduced-exertion high-intensity interval training (REHIT) improves V˙O2V˙O2 max in sedentary men and women and insulin sensitivity in men. Here, we present two studies examining the acute physiological and molecular responses to REHIT.MethodsIn Study 1...

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Published in: European Journal of Applied Physiology
ISSN: 1439-6319 1439-6327
Published: 2015
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URI: https://cronfa.swan.ac.uk/Record/cronfa35654
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spelling 2020-07-07T11:51:47.9126430 v2 35654 2017-09-26 Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT) 9bb783273dd9d54a2f3f66f75c43abdf 0000-0003-0980-2977 Richard Metcalfe Richard Metcalfe true false 2017-09-26 STSC PurposeWe have previously shown that 6 weeks of reduced-exertion high-intensity interval training (REHIT) improves V˙O2V˙O2 max in sedentary men and women and insulin sensitivity in men. Here, we present two studies examining the acute physiological and molecular responses to REHIT.MethodsIn Study 1, five men and six women (age: 26 ± 7 year, BMI: 23 ± 3 kg m−2, V˙O2V˙O2 max: 51 ± 11 ml kg−1 min−1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30, and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21 ± 2 year; 25 ± 4 kg·m−2; 39 ± 10 ml kg−1 min−1) performed three trials (REHIT, 30-min cycling at 50 % of V˙O2V˙O2 max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30, and 90 min post-exercise.ResultsAcutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V˙O2V˙O2 , RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.ConclusionsCollectively, these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise. Journal Article European Journal of Applied Physiology 115 11 2321 2334 1439-6319 1439-6327 HIT, Glycogen, Signalling pathways, AMPK, Exercise metabolism, Energy balance 1 11 2015 2015-11-01 10.1007/s00421-015-3217-6 https://link.springer.com/article/10.1007%2Fs00421-015-3217-6 COLLEGE NANME Sport and Exercise Sciences COLLEGE CODE STSC Swansea University 2020-07-07T11:51:47.9126430 2017-09-26T13:14:27.0233483 Faculty of Science and Engineering School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Sport and Exercise Sciences Richard Metcalfe 0000-0003-0980-2977 1 F. Koumanov 2 J. S. Ruffino 3 K. A. Stokes 4 G. D. Holman 5 D. Thompson 6 N. B. J. Vollaard 7 0035654-29092017094714.pdf Metcalfeetal(2015).pdf 2017-09-29T09:47:14.8630000 Output 756165 application/pdf Accepted Manuscript true 2017-09-29T00:00:00.0000000 false eng
title Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
spellingShingle Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
Richard Metcalfe
title_short Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
title_full Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
title_fullStr Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
title_full_unstemmed Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
title_sort Physiological and molecular responses to an acute bout of reduced-exertion high-intensity interval training (REHIT)
author_id_str_mv 9bb783273dd9d54a2f3f66f75c43abdf
author_id_fullname_str_mv 9bb783273dd9d54a2f3f66f75c43abdf_***_Richard Metcalfe
author Richard Metcalfe
author2 Richard Metcalfe
F. Koumanov
J. S. Ruffino
K. A. Stokes
G. D. Holman
D. Thompson
N. B. J. Vollaard
format Journal article
container_title European Journal of Applied Physiology
container_volume 115
container_issue 11
container_start_page 2321
publishDate 2015
institution Swansea University
issn 1439-6319
1439-6327
doi_str_mv 10.1007/s00421-015-3217-6
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Sport and Exercise Sciences{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Aerospace, Civil, Electrical, General and Mechanical Engineering - Sport and Exercise Sciences
url https://link.springer.com/article/10.1007%2Fs00421-015-3217-6
document_store_str 1
active_str 0
description PurposeWe have previously shown that 6 weeks of reduced-exertion high-intensity interval training (REHIT) improves V˙O2V˙O2 max in sedentary men and women and insulin sensitivity in men. Here, we present two studies examining the acute physiological and molecular responses to REHIT.MethodsIn Study 1, five men and six women (age: 26 ± 7 year, BMI: 23 ± 3 kg m−2, V˙O2V˙O2 max: 51 ± 11 ml kg−1 min−1) performed a single 10-min REHIT cycling session (60 W and two 20-s ‘all-out’ sprints), with vastus lateralis biopsies taken before and 0, 30, and 180 min post-exercise for analysis of glycogen content, phosphorylation of AMPK, p38 MAPK and ACC, and gene expression of PGC1α and GLUT4. In Study 2, eight men (21 ± 2 year; 25 ± 4 kg·m−2; 39 ± 10 ml kg−1 min−1) performed three trials (REHIT, 30-min cycling at 50 % of V˙O2V˙O2 max, and a resting control condition) in a randomised cross-over design. Expired air, venous blood samples, and subjective measures of appetite and fatigue were collected before and 0, 15, 30, and 90 min post-exercise.ResultsAcutely, REHIT was associated with a decrease in muscle glycogen, increased ACC phosphorylation, and activation of PGC1α. When compared to aerobic exercise, changes in V˙O2V˙O2 , RER, plasma volume, and plasma lactate and ghrelin were significantly more pronounced with REHIT, whereas plasma glucose, NEFAs, PYY, and measures of appetite were unaffected.ConclusionsCollectively, these data demonstrate that REHIT is associated with a pronounced disturbance of physiological homeostasis and associated activation of signalling pathways, which together may help explain previously observed adaptations once considered exclusive to aerobic exercise.
published_date 2015-11-01T03:44:25Z
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score 11.013731

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