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Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome

Virginia Post, Llinos Harris Orcid Logo, Mario Morgenstern, Leonardos Mageiros, Matthew Hitchings Orcid Logo, Guillaume Méric, Ben Pascoe, Samuel K. Sheppard, R. Geoff Richards, T. Fintan Moriarty

Journal of Clinical Microbiology, Volume: 55, Issue: 10, Pages: 3089 - 3103

Swansea University Authors: Llinos Harris Orcid Logo, Matthew Hitchings Orcid Logo

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DOI (Published version): 10.1128/JCM.00881-17

Abstract

Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected pa...

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Published in: Journal of Clinical Microbiology
ISSN: 0095-1137 1098-660X
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa35058
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spelling 2020-07-29T11:49:15.7259200 v2 35058 2017-08-30 Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 2017-08-30 BMS Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcomes were evaluated, after an average of 26 months, as either “cured” or “not cured.” The isolates were tested for antibiotic susceptibility and biofilm formation. Whole-genome sequencing was performed on all isolates, and genomic variation was related to features associated with “cured” and “not cured.” Strong biofilm formation and aminoglycoside resistance were associated with a “not-cured” outcome (P = 0.031 and P = 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the “not-cured” group. These included the biofilm-associated bhp gene, the antiseptic resistance qacA gene, the cassette chromosome recombinase-encoding genes ccrA and ccrB, and the IS256-like transposase gene. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole-genome sequencing identified specific genes associated with a “not-cured” outcome that should be validated in future studies. (The study has been registered at ClinicalTrials.govwith identifier NCT02640937.) Journal Article Journal of Clinical Microbiology 55 10 3089 3103 0095-1137 1098-660X Staphylococcus epidermidis; MRSE; virulence factors; antibiotic resistance; genotype; phenotype; orthopedic device-related infections 1 10 2017 2017-10-01 10.1128/JCM.00881-17 https://researchportal.bath.ac.uk/en/publications/a-comparative-genomics-study-of-istaphylococcus-epidermidisi-from COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2020-07-29T11:49:15.7259200 2017-08-30T11:00:46.7347476 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Virginia Post 1 Llinos Harris 0000-0002-0295-3038 2 Mario Morgenstern 3 Leonardos Mageiros 4 Matthew Hitchings 0000-0002-5527-4709 5 Guillaume Méric 6 Ben Pascoe 7 Samuel K. Sheppard 8 R. Geoff Richards 9 T. Fintan Moriarty 10
title Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
spellingShingle Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
Llinos Harris
Matthew Hitchings
title_short Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
title_full Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
title_fullStr Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
title_full_unstemmed Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
title_sort Comparative Genomics Study of Staphylococcus epidermidis Isolates from Orthopedic-Device-Related Infections Correlated with Patient Outcome
author_id_str_mv dc70f9d4badbbdb5d467fd321986d173
be98847c72c14a731c4a6b7bc02b3bcf
author_id_fullname_str_mv dc70f9d4badbbdb5d467fd321986d173_***_Llinos Harris
be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
author Llinos Harris
Matthew Hitchings
author2 Virginia Post
Llinos Harris
Mario Morgenstern
Leonardos Mageiros
Matthew Hitchings
Guillaume Méric
Ben Pascoe
Samuel K. Sheppard
R. Geoff Richards
T. Fintan Moriarty
format Journal article
container_title Journal of Clinical Microbiology
container_volume 55
container_issue 10
container_start_page 3089
publishDate 2017
institution Swansea University
issn 0095-1137
1098-660X
doi_str_mv 10.1128/JCM.00881-17
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url https://researchportal.bath.ac.uk/en/publications/a-comparative-genomics-study-of-istaphylococcus-epidermidisi-from
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description Staphylococcus epidermidis has emerged as an important opportunistic pathogen causing orthopedic-device-related infections (ODRI). This study investigated the association of genome variation and phenotypic features of the infecting S. epidermidis isolate with the clinical outcome for the infected patient. S. epidermidis isolates were collected from 104 patients with ODRI. Their clinical outcomes were evaluated, after an average of 26 months, as either “cured” or “not cured.” The isolates were tested for antibiotic susceptibility and biofilm formation. Whole-genome sequencing was performed on all isolates, and genomic variation was related to features associated with “cured” and “not cured.” Strong biofilm formation and aminoglycoside resistance were associated with a “not-cured” outcome (P = 0.031 and P = 0.001, respectively). Based on gene-by-gene analysis, some accessory genes were more prevalent in isolates from the “not-cured” group. These included the biofilm-associated bhp gene, the antiseptic resistance qacA gene, the cassette chromosome recombinase-encoding genes ccrA and ccrB, and the IS256-like transposase gene. This study identifies biofilm formation and antibiotic resistance as associated with poor outcome in S. epidermidis ODRI. Whole-genome sequencing identified specific genes associated with a “not-cured” outcome that should be validated in future studies. (The study has been registered at ClinicalTrials.govwith identifier NCT02640937.)
published_date 2017-10-01T03:43:30Z
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