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The effect of ventricular assist device-associated biomaterials on human blood leukocytes

Gemma Radley, Ina Laura Pieper, Cathy Thornton Orcid Logo

Journal of Biomedical Materials Research Part B: Applied Biomaterials

Swansea University Author: Cathy Thornton Orcid Logo

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DOI (Published version): 10.1002/jbm.b.33981

Abstract

Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the bi...

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Published in: Journal of Biomedical Materials Research Part B: Applied Biomaterials
ISSN: 15524973
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa34948
first_indexed 2017-08-21T18:50:38Z
last_indexed 2018-02-09T05:25:35Z
id cronfa34948
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spelling 2017-11-21T17:04:49.1717793 v2 34948 2017-08-21 The effect of ventricular assist device-associated biomaterials on human blood leukocytes c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 2017-08-21 MEDS Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices. Journal Article Journal of Biomedical Materials Research Part B: Applied Biomaterials 15524973 Ventricular Assist Devices; Biomaterials; Human; Blood; Flow Cytometry 9 9 2017 2017-09-09 10.1002/jbm.b.33981 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2017-11-21T17:04:49.1717793 2017-08-21T14:43:06.2953962 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Gemma Radley 1 Ina Laura Pieper 2 Cathy Thornton 0000-0002-5153-573X 3 0034948-12092017120257.pdf 20170831_GRadley_JBMRBAcceptedScript.pdf 2017-09-12T12:02:57.1300000 Output 689641 application/pdf Accepted Manuscript true 2018-09-12T00:00:00.0000000 true eng
title The effect of ventricular assist device-associated biomaterials on human blood leukocytes
spellingShingle The effect of ventricular assist device-associated biomaterials on human blood leukocytes
Cathy Thornton
title_short The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_full The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_fullStr The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_full_unstemmed The effect of ventricular assist device-associated biomaterials on human blood leukocytes
title_sort The effect of ventricular assist device-associated biomaterials on human blood leukocytes
author_id_str_mv c71a7a4be7361094d046d312202bce0c
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
author Cathy Thornton
author2 Gemma Radley
Ina Laura Pieper
Cathy Thornton
format Journal article
container_title Journal of Biomedical Materials Research Part B: Applied Biomaterials
publishDate 2017
institution Swansea University
issn 15524973
doi_str_mv 10.1002/jbm.b.33981
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
active_str 0
description Background: Ventricular assist devices (VADs) are an effective bridging or destination therapy for patients with advanced stage heart failure. These devices remain susceptible to adverse events including infection, bleeding, and thrombus; events linked to the foreign body response. Therefore, the biocompatibility of all biomaterials used is crucial to the success of medical devices.Methods: Biomaterials common in VADs - DLC: diamond-like carbon coated stainless steel; Sap: single-crystal sapphire; SiN: silicon nitride; Ti: titanium alloy; and ZTA: zirconia-toughened alumina - were tested for their biocompatibility through incubation with whole human blood for 2 hours with mild agitation. Blood was then removed and used for: complete cell counts; leukocyte activation and death, and the production of key inflammatory cytokines. All were compared to time 0 and an un-exposed 2 hour sample.Results: Monocyte numbers were lower after exposure to DLC, SiN and ZTA and monocytes showed evidence of activation with DLC, Sap, and SiN. Neutrophils and lymphocytes were unaffected.Conclusions: This approach allows comprehensive analysis of the potential blood damaging effects of biomaterials. Monocyte activation by DLC, Sap, ZTA and SiN warrants further investigation linking effects on this cell type to unfavourable inflammatory/thrombogenic responses to VADs and other blood handling devices.
published_date 2017-09-09T04:15:12Z
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score 11.3749895

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