Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Zhang, Yadan; Morgan, Ben J.; Smith, Rachel; Fellows, Christopher R.; Thornton, Cathy; Snow, Martyn; Francis, Lewis; Khan, Ilyas

doi:10.1038/s41598-017-02297-9

Journal article 1518 views 157 downloads

Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Yadan Zhang, Ben J. Morgan, Rachel Smith, Christopher R. Fellows, Cathy Thornton

, Martyn Snow, Lewis Francis

, Ilyas Khan

Scientific Reports, Volume: 7, Issue: 1

Swansea University Authors: Cathy Thornton , Lewis Francis , Ilyas Khan

PDF | Version of Record

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.
Download (1.94MB)

Check full text

DOI (Published version): 10.1038/s41598-017-02297-9

Abstract

Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re express phenotypic biomarkers of immature cartilage so tissue maturati...

Full description

Published in:	Scientific Reports
ISSN:	2045-2322
Published:	2017
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa33950
Tags:	Add Tag No Tags, Be the first to tag this record!

first_indexed	2017-05-26T15:02:35Z
last_indexed	2020-07-14T18:53:47Z
id	cronfa33950
recordtype	SURis
fullrecord	<?xml version="1.0"?><rfc1807><datestamp>2020-07-14T15:58:21.1882300</datestamp><bib-version>v2</bib-version><id>33950</id><entry>2017-05-26</entry><title>Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation</title><swanseaauthors><author><sid>c71a7a4be7361094d046d312202bce0c</sid><ORCID>0000-0002-5153-573X</ORCID><firstname>Cathy</firstname><surname>Thornton</surname><name>Cathy Thornton</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>10f61f9c1248951c1a33f6a89498f37d</sid><ORCID>0000-0002-7803-7714</ORCID><firstname>Lewis</firstname><surname>Francis</surname><name>Lewis Francis</name><active>true</active><ethesisStudent>false</ethesisStudent></author><author><sid>2536d955ff70e7b77063a8efe9103161</sid><ORCID>0000-0002-3886-1987</ORCID><firstname>Ilyas</firstname><surname>Khan</surname><name>Ilyas Khan</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2017-05-26</date><deptcode>BMS</deptcode><abstract>Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factorinduced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin1 upregulated. PRP increased cartilage surface cell density 1.5fold (P &#60; 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5fold increase in stiffness correlating with a 10fold upregulation of lysyl oxidase like1 gene expression (P &#60; 0.001). These data show PRP induces key aspects of postnatal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.</abstract><type>Journal Article</type><journal>Scientific Reports</journal><volume>7</volume><journalNumber>1</journalNumber><publisher/><issnElectronic>2045-2322</issnElectronic><keywords>Cartilage Platelet-rich plasma repair maturation</keywords><publishedDay>31</publishedDay><publishedMonth>12</publishedMonth><publishedYear>2017</publishedYear><publishedDate>2017-12-31</publishedDate><doi>10.1038/s41598-017-02297-9</doi><url/><notes/><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2020-07-14T15:58:21.1882300</lastEdited><Created>2017-05-26T11:57:42.4622453</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Yadan</firstname><surname>Zhang</surname><order>1</order></author><author><firstname>Ben J.</firstname><surname>Morgan</surname><order>2</order></author><author><firstname>Rachel</firstname><surname>Smith</surname><order>3</order></author><author><firstname>Christopher R.</firstname><surname>Fellows</surname><order>4</order></author><author><firstname>Cathy</firstname><surname>Thornton</surname><orcid>0000-0002-5153-573X</orcid><order>5</order></author><author><firstname>Martyn</firstname><surname>Snow</surname><order>6</order></author><author><firstname>Lewis</firstname><surname>Francis</surname><orcid>0000-0002-7803-7714</orcid><order>7</order></author><author><firstname>Ilyas</firstname><surname>Khan</surname><orcid>0000-0002-3886-1987</orcid><order>8</order></author></authors><documents><document><filename>0033950-28062017114403.pdf</filename><originalFilename>IK.s41598-017-02297-9.pdf</originalFilename><uploaded>2017-06-28T11:44:03.5770000</uploaded><type>Output</type><contentLength>2019037</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2017-06-28T00:00:00.0000000</embargoDate><documentNotes>This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source.</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling	2020-07-14T15:58:21.1882300 v2 33950 2017-05-26 Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 2017-05-26 BMS Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factorinduced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin1 upregulated. PRP increased cartilage surface cell density 1.5fold (P < 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5fold increase in stiffness correlating with a 10fold upregulation of lysyl oxidase like1 gene expression (P < 0.001). These data show PRP induces key aspects of postnatal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair. Journal Article Scientific Reports 7 1 2045-2322 Cartilage Platelet-rich plasma repair maturation 31 12 2017 2017-12-31 10.1038/s41598-017-02297-9 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2020-07-14T15:58:21.1882300 2017-05-26T11:57:42.4622453 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Yadan Zhang 1 Ben J. Morgan 2 Rachel Smith 3 Christopher R. Fellows 4 Cathy Thornton 0000-0002-5153-573X 5 Martyn Snow 6 Lewis Francis 0000-0002-7803-7714 7 Ilyas Khan 0000-0002-3886-1987 8 0033950-28062017114403.pdf IK.s41598-017-02297-9.pdf 2017-06-28T11:44:03.5770000 Output 2019037 application/pdf Version of Record true 2017-06-28T00:00:00.0000000 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source. true eng
title	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
spellingShingle	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation Cathy Thornton Lewis Francis Ilyas Khan
title_short	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_fullStr	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full_unstemmed	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_sort	Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
author_id_str_mv	c71a7a4be7361094d046d312202bce0c 10f61f9c1248951c1a33f6a89498f37d 2536d955ff70e7b77063a8efe9103161
author_id_fullname_str_mv	c71a7a4be7361094d046d312202bce0c_*_Cathy Thornton 10f61f9c1248951c1a33f6a89498f37d__Lewis Francis 2536d955ff70e7b77063a8efe9103161_**_Ilyas Khan
author	Cathy Thornton Lewis Francis Ilyas Khan
author2	Yadan Zhang Ben J. Morgan Rachel Smith Christopher R. Fellows Cathy Thornton Martyn Snow Lewis Francis Ilyas Khan
format	Journal article
container_title	Scientific Reports
container_volume	7
container_issue	1
publishDate	2017
institution	Swansea University
issn	2045-2322
doi_str_mv	10.1038/s41598-017-02297-9
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str	1
active_str	0
description	Platelet-rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factorinduced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin1 upregulated. PRP increased cartilage surface cell density 1.5fold (P < 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5fold increase in stiffness correlating with a 10fold upregulation of lysyl oxidase like1 gene expression (P < 0.001). These data show PRP induces key aspects of postnatal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.
published_date	2017-12-31T03:42:06Z
_version_	1763751940507828224
score	11.013148

Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Similar Items