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Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation

Yadan Zhang, Ben J. Morgan, Rachel Smith, Christopher R. Fellows, Cathy Thornton Orcid Logo, Martyn Snow, Lewis Francis Orcid Logo, Ilyas Khan Orcid Logo

Scientific Reports, Volume: 7, Issue: 1

Swansea University Authors: Cathy Thornton Orcid Logo, Lewis Francis Orcid Logo, Ilyas Khan Orcid Logo

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DOI (Published version): 10.1038/s41598-017-02297-9

Abstract

Platelet-­rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re­ express phenotypic biomarkers of immature cartilage so tissue maturati...

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Published in: Scientific Reports
ISSN: 2045-2322
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa33950
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last_indexed 2020-07-14T18:53:47Z
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Chondrocytes in injured and diseased situations frequently re&#xAD; express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor&#xAD;induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin&#xAD;1 upregulated. PRP increased cartilage surface cell density 1.5&#xAD;fold (P &amp;#60; 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5&#xAD;fold increase in stiffness correlating with a 10&#xAD;fold upregulation of lysyl oxidase like&#xAD;1 gene expression (P &amp;#60; 0.001). 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spelling 2020-07-14T15:58:21.1882300 v2 33950 2017-05-26 Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation c71a7a4be7361094d046d312202bce0c 0000-0002-5153-573X Cathy Thornton Cathy Thornton true false 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 2017-05-26 MEDS Platelet-­rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re­ express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor­induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin­1 upregulated. PRP increased cartilage surface cell density 1.5­fold (P &#60; 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5­fold increase in stiffness correlating with a 10­fold upregulation of lysyl oxidase like­1 gene expression (P &#60; 0.001). These data show PRP induces key aspects of post­natal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair. Journal Article Scientific Reports 7 1 2045-2322 Cartilage Platelet-rich plasma repair maturation 31 12 2017 2017-12-31 10.1038/s41598-017-02297-9 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2020-07-14T15:58:21.1882300 2017-05-26T11:57:42.4622453 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Yadan Zhang 1 Ben J. Morgan 2 Rachel Smith 3 Christopher R. Fellows 4 Cathy Thornton 0000-0002-5153-573X 5 Martyn Snow 6 Lewis Francis 0000-0002-7803-7714 7 Ilyas Khan 0000-0002-3886-1987 8 0033950-28062017114403.pdf IK.s41598-017-02297-9.pdf 2017-06-28T11:44:03.5770000 Output 2019037 application/pdf Version of Record true 2017-06-28T00:00:00.0000000 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source. true eng
title Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
spellingShingle Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
Cathy Thornton
Lewis Francis
Ilyas Khan
title_short Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_fullStr Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_full_unstemmed Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
title_sort Platelet-rich plasma induces post-natal maturation of immature articular cartilage and correlates with LOXL1 activation
author_id_str_mv c71a7a4be7361094d046d312202bce0c
10f61f9c1248951c1a33f6a89498f37d
2536d955ff70e7b77063a8efe9103161
author_id_fullname_str_mv c71a7a4be7361094d046d312202bce0c_***_Cathy Thornton
10f61f9c1248951c1a33f6a89498f37d_***_Lewis Francis
2536d955ff70e7b77063a8efe9103161_***_Ilyas Khan
author Cathy Thornton
Lewis Francis
Ilyas Khan
author2 Yadan Zhang
Ben J. Morgan
Rachel Smith
Christopher R. Fellows
Cathy Thornton
Martyn Snow
Lewis Francis
Ilyas Khan
format Journal article
container_title Scientific Reports
container_volume 7
container_issue 1
publishDate 2017
institution Swansea University
issn 2045-2322
doi_str_mv 10.1038/s41598-017-02297-9
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
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description Platelet-­rich plasma (PRP) is used to stimulate the repair of acute and chronic cartilage damage even though there is no definitive evidence of how this is achieved. Chondrocytes in injured and diseased situations frequently re­ express phenotypic biomarkers of immature cartilage so tissue maturation is a potential pathway for restoration of normal structure and function. We used an in vitro model of growth factor­induced maturation to perform a comparative study in order to determine whether PRP can also induce this specific form of remodeling that is characterised by increased cellular proliferation and tissue stiffness. Gene expression patterns specific for maturation were mimicked in PRP treated cartilage, with chondromodulin, collagen types II/X downregulated, deiodinase II and netrin­1 upregulated. PRP increased cartilage surface cell density 1.5­fold (P &#60; 0.05), confirmed by bromodeoxyuridine incorporation and proportionate increases in proliferating cell nuclear antigen gene expression. Atomic force microscopy analysis of PRP and growth factor treated cartilage gave a 5­fold increase in stiffness correlating with a 10­fold upregulation of lysyl oxidase like­1 gene expression (P &#60; 0.001). These data show PRP induces key aspects of post­natal maturation in immature cartilage and provides the basis to evaluate a new biological rationale for its activity when used clinically to initiate joint repair.
published_date 2017-12-31T04:12:51Z
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