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Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella

Vadim Y. Kryukov, Olga N. Yaroslavtseva, Miranda M. A. Whitten, Maksim V. Tyurin, Katherine Ficken, Carolyn Greig, Nadja R. Melo, Viktor V. Glupov, Ivan M. Dubovskiy, Tariq Butt Orcid Logo, Miranda Walker

Insect Science

Swansea University Authors: Katherine Ficken, Tariq Butt Orcid Logo, Miranda Walker

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DOI (Published version): 10.1111/1744-7917.12426

Abstract

This is an investigation of how temperature modulates the dynamics of fungus – insect interactions. The study focusses on the ability of the entomopathogenic fungus Metarhizium robertsii to infect the wax moth, Galleria mellonella, which is a well-studied and important model insect. Wax moth larvae...

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Published in: Insect Science
ISSN: 16729609
Published: 2017
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URI: https://cronfa.swan.ac.uk/Record/cronfa31997
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spelling 2017-03-16T14:39:06.4698508 v2 31997 2017-02-16 Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella 8f0edd50c22eb16f8e28f23ebc1177b5 Katherine Ficken Katherine Ficken true false 85d1c2ddde272a1176e74978e25ebece 0000-0002-8789-9543 Tariq Butt Tariq Butt true false 83389c57d000a704fae36fda87d7ca76 Miranda Walker Miranda Walker true false 2017-02-16 SGE This is an investigation of how temperature modulates the dynamics of fungus – insect interactions. The study focusses on the ability of the entomopathogenic fungus Metarhizium robertsii to infect the wax moth, Galleria mellonella, which is a well-studied and important model insect. Wax moth larvae in the wild develop optimally at around 34°C in beehives. However, surprisingly little research on wax moths has been conducted at temperatures relevant to the wild. Metarhizium robertsii was considerably more pathogenic at a constant temperature of 24°C compared with 34°C. The cooler temperature allowed greater condial adhesion to the cuticle, germination and haemocoel invasion. The wax moth larvae immune responses also altered with the temperature, and with the infective dose of the fungus. Enzyme-based immune defenses (lysozyme and phenoloxidase) exhibited enhanced activity at the warmer temperature. The predominant insect epicuticular fatty acids (palmetic, oleic and linoleic acids) were synthesised in greater quantities in larvae maintained at 24°C compared with those at 34°C, but these failed to exert fungistatic effects on topically applied fungus. It is clearly important to choose environmental conditions that are relevant to the habitat of the insect host when investigating the dynamics and outcome of insect / fungus interactions. The study has particular significance for the application of entomopathogens as biocontrol agents. Journal Article Insect Science 16729609 21 2 2017 2017-02-21 10.1111/1744-7917.12426 http://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12426/full COLLEGE NANME Geography COLLEGE CODE SGE Swansea University 2017-03-16T14:39:06.4698508 2017-02-16T10:56:50.0903157 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Vadim Y. Kryukov 1 Olga N. Yaroslavtseva 2 Miranda M. A. Whitten 3 Maksim V. Tyurin 4 Katherine Ficken 5 Carolyn Greig 6 Nadja R. Melo 7 Viktor V. Glupov 8 Ivan M. Dubovskiy 9 Tariq Butt 0000-0002-8789-9543 10 Miranda Walker 11 0031997-24022017102617.pdf Kryukovetal17.pdf 2017-02-24T10:26:17.4730000 Output 1371564 application/pdf Accepted Manuscript true 2017-11-29T00:00:00.0000000 true eng
title Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
spellingShingle Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
Katherine Ficken
Tariq Butt
Miranda Walker
title_short Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
title_full Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
title_fullStr Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
title_full_unstemmed Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
title_sort Fungal infection dynamics in response to temperature in the lepidopteran insect Galleria mellonella
author_id_str_mv 8f0edd50c22eb16f8e28f23ebc1177b5
85d1c2ddde272a1176e74978e25ebece
83389c57d000a704fae36fda87d7ca76
author_id_fullname_str_mv 8f0edd50c22eb16f8e28f23ebc1177b5_***_Katherine Ficken
85d1c2ddde272a1176e74978e25ebece_***_Tariq Butt
83389c57d000a704fae36fda87d7ca76_***_Miranda Walker
author Katherine Ficken
Tariq Butt
Miranda Walker
author2 Vadim Y. Kryukov
Olga N. Yaroslavtseva
Miranda M. A. Whitten
Maksim V. Tyurin
Katherine Ficken
Carolyn Greig
Nadja R. Melo
Viktor V. Glupov
Ivan M. Dubovskiy
Tariq Butt
Miranda Walker
format Journal article
container_title Insect Science
publishDate 2017
institution Swansea University
issn 16729609
doi_str_mv 10.1111/1744-7917.12426
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://onlinelibrary.wiley.com/doi/10.1111/1744-7917.12426/full
document_store_str 1
active_str 0
description This is an investigation of how temperature modulates the dynamics of fungus – insect interactions. The study focusses on the ability of the entomopathogenic fungus Metarhizium robertsii to infect the wax moth, Galleria mellonella, which is a well-studied and important model insect. Wax moth larvae in the wild develop optimally at around 34°C in beehives. However, surprisingly little research on wax moths has been conducted at temperatures relevant to the wild. Metarhizium robertsii was considerably more pathogenic at a constant temperature of 24°C compared with 34°C. The cooler temperature allowed greater condial adhesion to the cuticle, germination and haemocoel invasion. The wax moth larvae immune responses also altered with the temperature, and with the infective dose of the fungus. Enzyme-based immune defenses (lysozyme and phenoloxidase) exhibited enhanced activity at the warmer temperature. The predominant insect epicuticular fatty acids (palmetic, oleic and linoleic acids) were synthesised in greater quantities in larvae maintained at 24°C compared with those at 34°C, but these failed to exert fungistatic effects on topically applied fungus. It is clearly important to choose environmental conditions that are relevant to the habitat of the insect host when investigating the dynamics and outcome of insect / fungus interactions. The study has particular significance for the application of entomopathogens as biocontrol agents.
published_date 2017-02-21T03:39:07Z
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