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Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications

Owen Guy Orcid Logo, Z. Tehrani, D. J. Thomas, O. J. Guy, Zari Tehrani Orcid Logo

e-Journal of Surface Science and Nanotechnology, Volume: 14, Pages: 193 - 197

Swansea University Authors: Owen Guy Orcid Logo, Zari Tehrani Orcid Logo

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DOI (Published version): 10.1380/ejssnt.2016.193

Abstract

The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the anil...

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Published in: e-Journal of Surface Science and Nanotechnology
ISSN: 1348-0391
Published: 2016
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URI: https://cronfa.swan.ac.uk/Record/cronfa31387
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spelling 2016-12-08T10:48:36.2754545 v2 31387 2016-12-08 Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications c7fa5949b8528e048c5b978005f66794 0000-0002-6449-4033 Owen Guy Owen Guy true false fd8e614b01086804c80fbafa6fa6aaf5 0000-0002-5069-7921 Zari Tehrani Zari Tehrani true false 2016-12-08 CHEM The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the aniline mediator covalently bonded to electrode and biomarker. An electrochemical procedure to graft the mediator is described and the presence of biomarker at surface is demonstrated by using a fluorescence-labeled immune-reagent. An electrochemical functionalization process has been employed for attachment of functional aminie (NH2) linking groups to graphitic surfaces, which consists of two stages: (i) a reaction with a diazonium salt to covalently bond nitrobenzene groups to the surface and (ii) electrochemical reduction of the nitro group (–NO2) to an amine group (–NH2). The shape of the CV curve indicates that the redox reactions are taking place at the HOPG electrode surface. The amine group can subsequently be used to covalently link to an antibody biorecptor. The presence of 8-OHdG, indicative of DNA damage, has been linked to increased cancer risk. Detection of this oxidative stress biomarker is an important tool for the early diagnosis of disease. Journal Article e-Journal of Surface Science and Nanotechnology 14 0 193 197 1348-0391 31 12 2016 2016-12-31 10.1380/ejssnt.2016.193 COLLEGE NANME Chemistry COLLEGE CODE CHEM Swansea University 2016-12-08T10:48:36.2754545 2016-12-08T10:45:23.9877489 Faculty of Science and Engineering School of Engineering and Applied Sciences - Chemistry Owen Guy 0000-0002-6449-4033 1 Z. Tehrani 2 D. J. Thomas 3 O. J. Guy 4 Zari Tehrani 0000-0002-5069-7921 5 0031387-08122016104824.pdf tehrani2016(2).pdf 2016-12-08T10:48:24.5900000 Output 2537263 application/pdf Version of Record true 2016-12-08T00:00:00.0000000 false
title Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
spellingShingle Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
Owen Guy
Zari Tehrani
title_short Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
title_full Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
title_fullStr Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
title_full_unstemmed Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
title_sort Electrochemical Biofunctionalization of Highly Oriented Pyrolytic Graphite for Immunosensor Applications
author_id_str_mv c7fa5949b8528e048c5b978005f66794
fd8e614b01086804c80fbafa6fa6aaf5
author_id_fullname_str_mv c7fa5949b8528e048c5b978005f66794_***_Owen Guy
fd8e614b01086804c80fbafa6fa6aaf5_***_Zari Tehrani
author Owen Guy
Zari Tehrani
author2 Owen Guy
Z. Tehrani
D. J. Thomas
O. J. Guy
Zari Tehrani
format Journal article
container_title e-Journal of Surface Science and Nanotechnology
container_volume 14
container_issue 0
container_start_page 193
publishDate 2016
institution Swansea University
issn 1348-0391
doi_str_mv 10.1380/ejssnt.2016.193
college_str Faculty of Science and Engineering
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hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Chemistry{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Chemistry
document_store_str 1
active_str 0
description The present research demonstrates a procedure for surface modification of Highly Oriented Pyrolytic Graphite (HOPG) electrodes intended for use as immunosensors. The HOPG surface is linked to the molecule 8-hydroxydeoxyguanosine (8-OHdG), an oxidative stress biomarker for DNA damage, though the aniline mediator covalently bonded to electrode and biomarker. An electrochemical procedure to graft the mediator is described and the presence of biomarker at surface is demonstrated by using a fluorescence-labeled immune-reagent. An electrochemical functionalization process has been employed for attachment of functional aminie (NH2) linking groups to graphitic surfaces, which consists of two stages: (i) a reaction with a diazonium salt to covalently bond nitrobenzene groups to the surface and (ii) electrochemical reduction of the nitro group (–NO2) to an amine group (–NH2). The shape of the CV curve indicates that the redox reactions are taking place at the HOPG electrode surface. The amine group can subsequently be used to covalently link to an antibody biorecptor. The presence of 8-OHdG, indicative of DNA damage, has been linked to increased cancer risk. Detection of this oxidative stress biomarker is an important tool for the early diagnosis of disease.
published_date 2016-12-31T03:38:21Z
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