Journal article 938 views
Valorization of spent anaerobic digester effluents through production of platform chemicals using Clostridium butyricum
Myrto-panagiota Zacharof,
Clotilde Vouzelaud,
Stephen J. Mandale,
Robert Lovitt
Biomass and Bioenergy, Volume: 81, Pages: 294 - 303
Swansea University Authors: Myrto-panagiota Zacharof, Robert Lovitt
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DOI (Published version): 10.1016/j.biombioe.2015.07.018
Abstract
Spent digester streams were reformulated into nutrient rich effluents, capable of supporting biotechnological production of platform chemicals. This was achieved, using a set of physical pre-treatments including, sedimentation, dilution and sieving, followed by cross-flow microfiltration processing...
| Published in: | Biomass and Bioenergy |
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| Published: |
2015
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa28938 |
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| Abstract: |
Spent digester streams were reformulated into nutrient rich effluents, capable of supporting biotechnological production of platform chemicals. This was achieved, using a set of physical pre-treatments including, sedimentation, dilution and sieving, followed by cross-flow microfiltration processing to give particle and cell free nutrient streams with a C:N:P molar ratio of 1:1.35:0.03. These streams were inoculated on bench scale, with Clostridium butyricum NCIMB 7432, a well-known acids producer, giving good growth rates (μmax 0.24 h−1) and acids concentrations of 110 mM acetic acid and 18 mM per litre butyric acid. When the treated effluent was fortified with 2% w/v glucose there was a significant improvement with a 0.38 h−1 μmax and platform chemicals concentration of 279 mM acetic acid and 32 mM butyric acid per litre. These media gave comparable performance with the synthetic media, therefore demonstrating a valid alternative solution to commercial media preparations. The cost of treating the excess digestate by microfiltration within the scope of formulation to nutrient media has been calculated at 0.0033 $USD/kg, 3 times lower than the cost of production of nutrient media. |
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| College: |
Faculty of Science and Engineering |
| Start Page: |
294 |
| End Page: |
303 |