Journal article 1279 views 430 downloads
Epitaxial graphene immunosensor for human chorionic gonadotropin
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Steve Conlan ,
Deya Gonzalez ,
Sofia Rodrigues Teixeira
Sensors and Actuators B: Chemical, Volume: 190, Pages: 723 - 729
Swansea University Authors:
Owen Guy , Steve Conlan , Deya Gonzalez , Sofia Rodrigues Teixeira
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DOI (Published version): 10.1016/j.snb.2013.09.019
Abstract
Human chorionic gonadotropin (hCG), a 37 kDa glycoprotein hormone, is a key diagnostic marker of pregnancy and has been cited as an important biomarker in relation to cancerous tumors found in the prostate, ovaries and bladder.A novel chemically-modified epitaxial graphene diagnostic sensor has been...
| Published in: | Sensors and Actuators B: Chemical |
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| Published: |
2014
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa28115 |
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| Abstract: |
Human chorionic gonadotropin (hCG), a 37 kDa glycoprotein hormone, is a key diagnostic marker of pregnancy and has been cited as an important biomarker in relation to cancerous tumors found in the prostate, ovaries and bladder.A novel chemically-modified epitaxial graphene diagnostic sensor has been developed for ultrasensitive detection of the biomarker hCG. Multi-layer epitaxial graphene (MEG), grown on silicon carbide substrates, was patterned using electron beam lithography to produce channel based devices. The MEG channels have been amine terminated using 3-Aminopropyl-triethoxysilane (APTES) in order to attach the anti-hCG antibody to the channel.Detection of binding of hCG with its graphene-bound antibody was monitored by measuring reduction of the channel current of the graphene biosensor. The sensitivity of the sensor device was investigated using varying concentrations of hCG, with changes in the channel resistance of the sensor observed upon exposure to hCG. The detection limit of the sensor was 0.62 ng/mL and the sensor showed a linear response to hCG in the range 0.62–5.62 ng/mL with a response of 142 Ω/ng/mL. At concentrations above 5.62 ng/mL the sensor begins to saturate. |
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| College: |
Faculty of Science and Engineering |
| Start Page: |
723 |
| End Page: |
729 |