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Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon

Matthew Hitchings Orcid Logo

European Congress of Clinical Microbiology and Infectious Diseases 2016

Swansea University Author: Matthew Hitchings Orcid Logo

Abstract

Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different wi...

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Published in: European Congress of Clinical Microbiology and Infectious Diseases 2016
Published: 2016
Online Access: http://eccmidlive.org/#resources/disease-severity-associated-genomic-variation-among-campylobacter-jejuni-isolates-from-asymptomatic-and-acute-infection-in-children-of-the-peruvian-amazon
URI: https://cronfa.swan.ac.uk/Record/cronfa27687
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fullrecord <?xml version="1.0"?><rfc1807><datestamp>2017-01-19T15:47:00.0326911</datestamp><bib-version>v2</bib-version><id>27687</id><entry>2016-05-04</entry><title>Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon</title><swanseaauthors><author><sid>be98847c72c14a731c4a6b7bc02b3bcf</sid><ORCID>0000-0002-5527-4709</ORCID><firstname>Matthew</firstname><surname>Hitchings</surname><name>Matthew Hitchings</name><active>true</active><ethesisStudent>false</ethesisStudent></author></swanseaauthors><date>2016-05-04</date><deptcode>BMS</deptcode><abstract>Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different with asymptomatic unreported infection endemic in children younger than 2 years old. Asymptomatic infection is associated with poor cognitive and physical development.Material/methods: We correlate virulence and host cell response in in vitro assays with a detailed record of patient symptoms for in 101 C. jejuni isolates from a cohort of 442 children aged 0&#x2013;72 months. Isolates were sequenced and genome-wide association studies were used to identify genetic elements associated with asymptomatic carriage and specific host immune responses.Results: Genome-wide association studies identified a strong association between asymptomatic carriage and putative glycosylation, motility, capsule production and iron homeostasis genes. Laboratory work is ongoing to test the specific role of these genes in phenotypes relevant to host infection, including cytokine response, invasion and growth under iron depleted conditions.Conclusions: Improvements in the understanding of disease and carriage of C. jejnui has implications for current campylobacteriosis treatment regimens, vaccine design and the development of rapid diagnostics for early detection at the point of care.</abstract><type>Conference Paper/Proceeding/Abstract</type><journal>European Congress of Clinical Microbiology and Infectious Diseases 2016</journal><publisher/><keywords/><publishedDay>9</publishedDay><publishedMonth>4</publishedMonth><publishedYear>2016</publishedYear><publishedDate>2016-04-09</publishedDate><doi/><url>http://eccmidlive.org/#resources/disease-severity-associated-genomic-variation-among-campylobacter-jejuni-isolates-from-asymptomatic-and-acute-infection-in-children-of-the-peruvian-amazon</url><notes>Poster presentation.</notes><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2017-01-19T15:47:00.0326911</lastEdited><Created>2016-05-04T15:18:28.5091331</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Matthew</firstname><surname>Hitchings</surname><orcid>0000-0002-5527-4709</orcid><order>1</order></author></authors><documents/><OutputDurs/></rfc1807>
spelling 2017-01-19T15:47:00.0326911 v2 27687 2016-05-04 Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon be98847c72c14a731c4a6b7bc02b3bcf 0000-0002-5527-4709 Matthew Hitchings Matthew Hitchings true false 2016-05-04 BMS Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different with asymptomatic unreported infection endemic in children younger than 2 years old. Asymptomatic infection is associated with poor cognitive and physical development.Material/methods: We correlate virulence and host cell response in in vitro assays with a detailed record of patient symptoms for in 101 C. jejuni isolates from a cohort of 442 children aged 0–72 months. Isolates were sequenced and genome-wide association studies were used to identify genetic elements associated with asymptomatic carriage and specific host immune responses.Results: Genome-wide association studies identified a strong association between asymptomatic carriage and putative glycosylation, motility, capsule production and iron homeostasis genes. Laboratory work is ongoing to test the specific role of these genes in phenotypes relevant to host infection, including cytokine response, invasion and growth under iron depleted conditions.Conclusions: Improvements in the understanding of disease and carriage of C. jejnui has implications for current campylobacteriosis treatment regimens, vaccine design and the development of rapid diagnostics for early detection at the point of care. Conference Paper/Proceeding/Abstract European Congress of Clinical Microbiology and Infectious Diseases 2016 9 4 2016 2016-04-09 http://eccmidlive.org/#resources/disease-severity-associated-genomic-variation-among-campylobacter-jejuni-isolates-from-asymptomatic-and-acute-infection-in-children-of-the-peruvian-amazon Poster presentation. COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2017-01-19T15:47:00.0326911 2016-05-04T15:18:28.5091331 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Matthew Hitchings 0000-0002-5527-4709 1
title Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
spellingShingle Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
Matthew Hitchings
title_short Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
title_full Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
title_fullStr Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
title_full_unstemmed Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
title_sort Disease severity associated genomic variation among Campylobacter jejuni isolates from asymptomatic and acute infection in children of the Peruvian Amazon
author_id_str_mv be98847c72c14a731c4a6b7bc02b3bcf
author_id_fullname_str_mv be98847c72c14a731c4a6b7bc02b3bcf_***_Matthew Hitchings
author Matthew Hitchings
author2 Matthew Hitchings
format Conference Paper/Proceeding/Abstract
container_title European Congress of Clinical Microbiology and Infectious Diseases 2016
publishDate 2016
institution Swansea University
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://eccmidlive.org/#resources/disease-severity-associated-genomic-variation-among-campylobacter-jejuni-isolates-from-asymptomatic-and-acute-infection-in-children-of-the-peruvian-amazon
document_store_str 0
active_str 0
description Background: Accute Campylobacter gastroenteritis in developed countries is characterized by sporadic infection, peaking during infancy and early adulthood, with most infections resulting from consumption of contaminated food. In developing countries, the epidemiology of disease is quite different with asymptomatic unreported infection endemic in children younger than 2 years old. Asymptomatic infection is associated with poor cognitive and physical development.Material/methods: We correlate virulence and host cell response in in vitro assays with a detailed record of patient symptoms for in 101 C. jejuni isolates from a cohort of 442 children aged 0–72 months. Isolates were sequenced and genome-wide association studies were used to identify genetic elements associated with asymptomatic carriage and specific host immune responses.Results: Genome-wide association studies identified a strong association between asymptomatic carriage and putative glycosylation, motility, capsule production and iron homeostasis genes. Laboratory work is ongoing to test the specific role of these genes in phenotypes relevant to host infection, including cytokine response, invasion and growth under iron depleted conditions.Conclusions: Improvements in the understanding of disease and carriage of C. jejnui has implications for current campylobacteriosis treatment regimens, vaccine design and the development of rapid diagnostics for early detection at the point of care.
published_date 2016-04-09T03:33:38Z
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score 11.014358

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