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Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy

Zhaokai Meng, Sandra C. Bustamante Lopez, Kenith Meissner, Vladislav V. Yakovlev

Journal of Biophotonics, Volume: 9, Issue: 3, Pages: 201 - 207

Swansea University Author: Kenith Meissner

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DOI (Published version): 10.1002/jbio.201500163

Abstract

Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it...

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Published in: Journal of Biophotonics
Published: 2016
Online Access: http://onlinelibrary.wiley.com/doi/10.1002/jbio.201500163/abstract
URI: https://cronfa.swan.ac.uk/Record/cronfa27487
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first_indexed 2016-04-28T01:13:14Z
last_indexed 2019-08-01T21:20:41Z
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spelling 2019-08-01T16:41:03.0010138 v2 27487 2016-04-27 Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy 30fdfec0d8b19b59b57a818e054d4af3 Kenith Meissner Kenith Meissner true false 2016-04-27 FGSEN Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular- and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy. Journal Article Journal of Biophotonics 9 3 201 207 31 3 2016 2016-03-31 10.1002/jbio.201500163 http://onlinelibrary.wiley.com/doi/10.1002/jbio.201500163/abstract Published in a leading biomedical optics journal (Impact factor: 4.447), this work represents the first combined Raman/Brillouin study of red blood cell biochemistry/mechanical properties. The power in the combined techniques emerges from the fact that both techniques do not require tagging of the cells. The research from this developing international (Yakovlev, Texas A&M University, USA) collaboration was highlighted in our recently awarded EPSRC Platform grant proposal, “Engineering Blood Diagnostics: Integrated Platforms for Advanced Detection and Analysis.” Results from this work have been presented at international conferences was well as invited seminars at Imperial College and the University of Stuttgart. COLLEGE NANME Science and Engineering - Faculty COLLEGE CODE FGSEN Swansea University 2019-08-01T16:41:03.0010138 2016-04-27T16:40:52.3273994 Faculty of Science and Engineering School of Engineering and Applied Sciences - Uncategorised Zhaokai Meng 1 Sandra C. Bustamante Lopez 2 Kenith Meissner 3 Vladislav V. Yakovlev 4
title Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
spellingShingle Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
Kenith Meissner
title_short Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
title_full Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
title_fullStr Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
title_full_unstemmed Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
title_sort Subcellular measurements of mechanical and chemical properties using dual Raman-Brillouin microspectroscopy
author_id_str_mv 30fdfec0d8b19b59b57a818e054d4af3
author_id_fullname_str_mv 30fdfec0d8b19b59b57a818e054d4af3_***_Kenith Meissner
author Kenith Meissner
author2 Zhaokai Meng
Sandra C. Bustamante Lopez
Kenith Meissner
Vladislav V. Yakovlev
format Journal article
container_title Journal of Biophotonics
container_volume 9
container_issue 3
container_start_page 201
publishDate 2016
institution Swansea University
doi_str_mv 10.1002/jbio.201500163
college_str Faculty of Science and Engineering
hierarchytype
hierarchy_top_id facultyofscienceandengineering
hierarchy_top_title Faculty of Science and Engineering
hierarchy_parent_id facultyofscienceandengineering
hierarchy_parent_title Faculty of Science and Engineering
department_str School of Engineering and Applied Sciences - Uncategorised{{{_:::_}}}Faculty of Science and Engineering{{{_:::_}}}School of Engineering and Applied Sciences - Uncategorised
url http://onlinelibrary.wiley.com/doi/10.1002/jbio.201500163/abstract
document_store_str 0
active_str 0
description Brillouin microspectroscopy is a powerful technique for noninvasive optical imaging. In particular, Brillouin microspectroscopy uniquely allows assessing a sample's mechanical properties with microscopic spatial resolution. Recent advances in background-free Brillouin microspectroscopy make it possible to image scattering samples without substantial degradation of the data quality. However, measurements at the cellular- and subcellular-level have never been performed to date due to the limited signal strength. In this report, by adopting our recently optimized VIPA-based Brillouin spectrometer, we probed the microscopic viscoelasticity of individual red blood cells. These measurements were supplemented by chemically specific measurements using Raman microspectroscopy.
published_date 2016-03-31T03:33:19Z
_version_ 1763751388257452032
score 11.013148

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