New methods for analysis of oxysterols and related compounds by LC–MS

Griffiths, William; Abdel-Khalik, Jonas; Crick, Peter J.; Yutuc, Eylan; Wang, Yuqin

doi:10.1016/j.jsbmb.2015.11.017

Journal article 1468 views 237 downloads

New methods for analysis of oxysterols and related compounds by LC–MS

William Griffiths

, Jonas Abdel-Khalik, Peter J. Crick, Eylan Yutuc

, Yuqin Wang

The Journal of Steroid Biochemistry and Molecular Biology, Volume: 162, Pages: 4 - 26

Swansea University Authors: William Griffiths , Eylan Yutuc

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DOI (Published version): 10.1016/j.jsbmb.2015.11.017

Abstract

Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors...

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Published in:	The Journal of Steroid Biochemistry and Molecular Biology
ISSN:	09600760
Published:	2016
Online Access:	Check full text
URI:	https://cronfa.swan.ac.uk/Record/cronfa25192
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first_indexed	2015-12-22T01:52:40Z
last_indexed	2020-05-22T18:35:52Z
id	cronfa25192
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spelling	2020-05-22T18:50:39.7345877 v2 25192 2015-12-21 New methods for analysis of oxysterols and related compounds by LC–MS 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 99332f073ce913a9b7d8b6441b17516d 0000-0001-9971-1950 Eylan Yutuc Eylan Yutuc true false 2015-12-21 BMS Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors and also regulators of the processing of steroid regulatory element-binding proteins (SREBPs) to their active forms as transcription factors regulating cholesterol and fatty acid biosynthesis. Oxysterols are implicated in the pathogenesis of multiple disease states ranging from atherosclerosis and cancer to multiple sclerosis and other neurodegenerative diseases including Alzheimer’s and Parkinson’s disease. Analysis of oxysterols is challenging on account of their low abundance in biological systems in comparison to cholesterol, and due to the propensity of cholesterol to undergo oxidation in air to generate oxysterols with the same structures as those present endogenously. In this article we review the mass spectrometry-based methods for oxysterol analysis paying particular attention to analysis by liquid chromatography – mass spectrometry (LC-MS). Journal Article The Journal of Steroid Biochemistry and Molecular Biology 162 4 26 09600760 1 9 2016 2016-09-01 10.1016/j.jsbmb.2015.11.017 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University RCUK, BBSRC, BB/I001735/1 2020-05-22T18:50:39.7345877 2015-12-21T09:57:00.5733669 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine William Griffiths 0000-0002-4129-6616 1 Jonas Abdel-Khalik 2 Peter J. Crick 3 Eylan Yutuc 0000-0001-9971-1950 4 Yuqin Wang 5 0025192-09012017151047.pdf griffiths.1-s2.0-S0960076015301436-main.pdf 2017-01-09T15:10:47.4970000 Output 2320478 application/pdf Version of Record true 2017-01-09T00:00:00.0000000 Distributed under the terms of a CC-BY license. true
title	New methods for analysis of oxysterols and related compounds by LC–MS
spellingShingle	New methods for analysis of oxysterols and related compounds by LC–MS William Griffiths Eylan Yutuc
title_short	New methods for analysis of oxysterols and related compounds by LC–MS
title_full	New methods for analysis of oxysterols and related compounds by LC–MS
title_fullStr	New methods for analysis of oxysterols and related compounds by LC–MS
title_full_unstemmed	New methods for analysis of oxysterols and related compounds by LC–MS
title_sort	New methods for analysis of oxysterols and related compounds by LC–MS
author_id_str_mv	3316b1d1b524be1831790933eed1c26e 99332f073ce913a9b7d8b6441b17516d
author_id_fullname_str_mv	3316b1d1b524be1831790933eed1c26e_*_William Griffiths 99332f073ce913a9b7d8b6441b17516d_*_Eylan Yutuc
author	William Griffiths Eylan Yutuc
author2	William Griffiths Jonas Abdel-Khalik Peter J. Crick Eylan Yutuc Yuqin Wang
format	Journal article
container_title	The Journal of Steroid Biochemistry and Molecular Biology
container_volume	162
container_start_page	4
publishDate	2016
institution	Swansea University
issn	09600760
doi_str_mv	10.1016/j.jsbmb.2015.11.017
college_str	Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str	1
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description	Oxysterols are oxygenated forms of cholesterol or its precursors. They are formed enzymatically and via reactive oxygen species. Oxysterols are intermediates in bile acid and steroid hormone biosynthetic pathways and are also bioactive molecules in their own right, being ligands to nuclear receptors and also regulators of the processing of steroid regulatory element-binding proteins (SREBPs) to their active forms as transcription factors regulating cholesterol and fatty acid biosynthesis. Oxysterols are implicated in the pathogenesis of multiple disease states ranging from atherosclerosis and cancer to multiple sclerosis and other neurodegenerative diseases including Alzheimer’s and Parkinson’s disease. Analysis of oxysterols is challenging on account of their low abundance in biological systems in comparison to cholesterol, and due to the propensity of cholesterol to undergo oxidation in air to generate oxysterols with the same structures as those present endogenously. In this article we review the mass spectrometry-based methods for oxysterol analysis paying particular attention to analysis by liquid chromatography – mass spectrometry (LC-MS).
published_date	2016-09-01T03:30:01Z
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New methods for analysis of oxysterols and related compounds by LC–MS

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