Journal article 1370 views
In Vitro Biochemical Study of CYP51-Mediated Azole Resistance in Aspergillus fumigatus
,
W. David Nes,
Steven Kelly,
Diane Kelly
Antimicrobial Agents and Chemotherapy, Volume: 59, Issue: 12, Pages: 7771 - 7778
Swansea University Authors:
Josie Parker, Claire Price , Steven Kelly, Diane Kelly
Full text not available from this repository: check for access using links below.
DOI (Published version): 10.1128/aac.01806-15
Abstract
New classes of azole-based drugs are required to combat the increasing resistance to existing triazole therapeutics. In this study, a CYP51 reconstitution assay is described consisting of eburicol, purified recombinant Aspergillus fumigatus CPR1 (AfCPR1), and Escherichia coli membrane suspensions co...
| Published in: | Antimicrobial Agents and Chemotherapy |
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| ISSN: | 0066-4804 1098-6596 |
| Published: |
American Society for Microbiology
2015
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| Online Access: |
Check full text
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa23614 |
| Abstract: |
New classes of azole-based drugs are required to combat the increasing resistance to existing triazole therapeutics. In this study, a CYP51 reconstitution assay is described consisting of eburicol, purified recombinant Aspergillus fumigatus CPR1 (AfCPR1), and Escherichia coli membrane suspensions containing recombinant A. fumigatus CYP51 proteins, allowing in vitro screening of azole antifungals. Our AfCPR1/Af51 assay system demonstrated the biochemical basis for the increased azole resistance of A. fumigatus strains harboring G54W, L98H, and M220K Af51A point mutations. |
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| Keywords: |
antifungal; Aspergillus fumigatus; CYP 51; CPR; POINT MUTATIONS |
| College: |
Faculty of Medicine, Health and Life Sciences |
| Issue: |
12 |
| Start Page: |
7771 |
| End Page: |
7778 |