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Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment

Enrico Andreoli, Rei Suzuki, Alvin W. Orbaek, Manoop S. Bhutani, Robert H. Hauge, Wade Adams, Jason B. Fleming, Andrew Barron Orcid Logo, Alvin Orbaek White Orcid Logo

Journal of Materials Chemistry B, Volume: 2, Issue: 29, Pages: 4740 - 4747

Swansea University Authors: Andrew Barron Orcid Logo, Alvin Orbaek White Orcid Logo

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DOI (Published version): 10.1039/C4TB00778F

Abstract

High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then t...

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Published in: Journal of Materials Chemistry B
ISSN: 2050-750X 2050-7518
Published: 2014
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URI: https://cronfa.swan.ac.uk/Record/cronfa22210
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spelling 2020-06-10T14:41:19.7031581 v2 22210 2015-06-26 Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment 92e452f20936d688d36f91c78574241d 0000-0002-2018-8288 Andrew Barron Andrew Barron true false 8414a23650d4403fdfe1a735dbd2e24e 0000-0001-6338-5970 Alvin Orbaek White Alvin Orbaek White true false 2015-06-26 CHEG High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells. Journal Article Journal of Materials Chemistry B 2 29 4740 4747 2050-750X 2050-7518 7 8 2014 2014-08-07 10.1039/C4TB00778F http://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/c4tb00778f#!divAbstract COLLEGE NANME Chemical Engineering COLLEGE CODE CHEG Swansea University 2020-06-10T14:41:19.7031581 2015-06-26T16:18:21.9269522 Enrico Andreoli 1 Rei Suzuki 2 Alvin W. Orbaek 3 Manoop S. Bhutani 4 Robert H. Hauge 5 Wade Adams 6 Jason B. Fleming 7 Andrew Barron 0000-0002-2018-8288 8 Alvin Orbaek White 0000-0001-6338-5970 9
title Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
spellingShingle Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
Andrew Barron
Alvin Orbaek White
title_short Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
title_full Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
title_fullStr Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
title_full_unstemmed Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
title_sort Preparation and evaluation of polyethyleneimine-single walled carbon nanotube conjugates as vectors for pancreatic cancer treatment
author_id_str_mv 92e452f20936d688d36f91c78574241d
8414a23650d4403fdfe1a735dbd2e24e
author_id_fullname_str_mv 92e452f20936d688d36f91c78574241d_***_Andrew Barron
8414a23650d4403fdfe1a735dbd2e24e_***_Alvin Orbaek White
author Andrew Barron
Alvin Orbaek White
author2 Enrico Andreoli
Rei Suzuki
Alvin W. Orbaek
Manoop S. Bhutani
Robert H. Hauge
Wade Adams
Jason B. Fleming
Andrew Barron
Alvin Orbaek White
format Journal article
container_title Journal of Materials Chemistry B
container_volume 2
container_issue 29
container_start_page 4740
publishDate 2014
institution Swansea University
issn 2050-750X
2050-7518
doi_str_mv 10.1039/C4TB00778F
url http://pubs.rsc.org/en/Content/ArticleLanding/2014/TB/c4tb00778f#!divAbstract
document_store_str 0
active_str 0
description High quality single-walled carbon nanotubes (SWNTs) were obtained following a new purification procedure, based on using Cl2 gas at high temperature. Cl2-treated SWNTs were fluorinated and modified with branched polyethyleneimine (PEI) to afford covalently functionalised PEI–SWNTs, which were then tested for cytotoxicity both in vitro (HPNE and BxPC3 pancreatic cell lines) and in vivo (BxPC3 xenografts from nude mice) to establish that functionalization with lower molecular weight PEI (600 and 1800 Da) achieved higher cell viability in MTT assay. A shortened version of the nanotubes, PEI(1800)-cut-SWNT (1800 Da branched PEI), was also prepared and tested for cellular internalization in the BxPC3 adenocarcinoma cell line. Laser confocal imaging of the cells after incubation in the presence of RhoB-PEI(1800)-cut-SWNT (covalently labelled with rhodamine B) indicates that the PEI(1800)-cut-SWNTs can reach both the cytoplasm and nucleus of pancreatic cancer cells.
published_date 2014-08-07T03:26:25Z
_version_ 1763750954567467008
score 11.037056

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