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Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor

Dimitrios Konstantinou, Ming Lei, Zhidao Xia Orcid Logo, Venkat Kanamarlapudi Orcid Logo

Cell Biology International, Volume: 39, Issue: 4, Pages: 502 - 507

Swansea University Authors: Zhidao Xia Orcid Logo, Venkat Kanamarlapudi Orcid Logo

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DOI (Published version): 10.1002/cbin.10409

Abstract

Heart disease is the major leading cause of death worldwide and the use of stem cells promises new ways for its treatment. The relative easy and quick acquisition of human umbilical cord matrix mesenchymal stem cells (HUMSCs) and their properties make them useful for the treatment of cardiac disease...

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Published in: Cell Biology International
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa19760
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spelling 2019-06-20T17:12:44.8787470 v2 19760 2014-12-12 Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor c9307abfed1b43987a19da0c0e30d7a4 0000-0002-2047-7282 Zhidao Xia Zhidao Xia true false 63741801137148abfa4c00cd547dcdfa 0000-0002-8739-1483 Venkat Kanamarlapudi Venkat Kanamarlapudi true false 2014-12-12 BMS Heart disease is the major leading cause of death worldwide and the use of stem cells promises new ways for its treatment. The relative easy and quick acquisition of human umbilical cord matrix mesenchymal stem cells (HUMSCs) and their properties make them useful for the treatment of cardiac diseases. Therefore, the main aim of this investigation was to create cardiac polymicrotissue from HUMSCs using a combination of growth factors [sphingosine-1-phosphate (S1P) and suramin] and techniques (hanging drop and bioreactor). Using designated culture conditions of the growth factors (100 nM S1P and 500 µM suramin), cardiomyocyte differentiation medium (CDM), hanging drop, bioreactor and differentiation for 7 days, a potential specific cardiac polymicrotissue was derived from HUMSCs. The effectiveness of growth factors alone or in combination in differentiation of HUMSCs to cardiac polymicrotissue was analysed by assessing the presence of cardiac markers by immunocytochemistry. This analysis demonstrated the importance of those growth factors for the differentiation. This study for the first time demonstrated the formation of a cardiac polymicrotissue under specific culture conditions. The polymicrotissue, thus, obtained may be used in future as a 'patch' to cover the injured cardiac region and thereby useful for the treatment of heart diseases. Journal Article Cell Biology International 39 4 502 507 9 12 2014 2014-12-09 10.1002/cbin.10409 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-06-20T17:12:44.8787470 2014-12-12T17:41:13.5338894 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Dimitrios Konstantinou 1 Ming Lei 2 Zhidao Xia 0000-0002-2047-7282 3 Venkat Kanamarlapudi 0000-0002-8739-1483 4
title Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
spellingShingle Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
Zhidao Xia
Venkat Kanamarlapudi
title_short Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
title_full Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
title_fullStr Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
title_full_unstemmed Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
title_sort Growth factors mediated differentiation of mesenchymal stem cells to cardiac polymicrotissue using hanging drop and bioreactor
author_id_str_mv c9307abfed1b43987a19da0c0e30d7a4
63741801137148abfa4c00cd547dcdfa
author_id_fullname_str_mv c9307abfed1b43987a19da0c0e30d7a4_***_Zhidao Xia
63741801137148abfa4c00cd547dcdfa_***_Venkat Kanamarlapudi
author Zhidao Xia
Venkat Kanamarlapudi
author2 Dimitrios Konstantinou
Ming Lei
Zhidao Xia
Venkat Kanamarlapudi
format Journal article
container_title Cell Biology International
container_volume 39
container_issue 4
container_start_page 502
publishDate 2014
institution Swansea University
doi_str_mv 10.1002/cbin.10409
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Heart disease is the major leading cause of death worldwide and the use of stem cells promises new ways for its treatment. The relative easy and quick acquisition of human umbilical cord matrix mesenchymal stem cells (HUMSCs) and their properties make them useful for the treatment of cardiac diseases. Therefore, the main aim of this investigation was to create cardiac polymicrotissue from HUMSCs using a combination of growth factors [sphingosine-1-phosphate (S1P) and suramin] and techniques (hanging drop and bioreactor). Using designated culture conditions of the growth factors (100 nM S1P and 500 µM suramin), cardiomyocyte differentiation medium (CDM), hanging drop, bioreactor and differentiation for 7 days, a potential specific cardiac polymicrotissue was derived from HUMSCs. The effectiveness of growth factors alone or in combination in differentiation of HUMSCs to cardiac polymicrotissue was analysed by assessing the presence of cardiac markers by immunocytochemistry. This analysis demonstrated the importance of those growth factors for the differentiation. This study for the first time demonstrated the formation of a cardiac polymicrotissue under specific culture conditions. The polymicrotissue, thus, obtained may be used in future as a 'patch' to cover the injured cardiac region and thereby useful for the treatment of heart diseases.
published_date 2014-12-09T03:23:16Z
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score 11.037056

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