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Endometrial cells sense and react to tissue damage during infection of the bovine endometrium via interleukin 1
,
James Cronin ,
I. Martin Sheldon,
Martin Sheldon
Scientific Reports, Volume: 4, Start page: 7060
Swansea University Authors:
Laura Healy , James Cronin , Martin Sheldon
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DOI (Published version): 10.1038/srep07060
Abstract
Cells generate inflammatory responses to bacteria when pattern recognition receptors bind pathogen-associated molecules such as lipopolysaccharide. Cells may also respond to tissue damage by sensing damage-associated molecules. Postpartum bacterial infections of the bovine uterus cause endometritis...
| Published in: | Scientific Reports |
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| Published: |
2014
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| URI: | https://cronfa.swan.ac.uk/Record/cronfa19258 |
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| Abstract: |
Cells generate inflammatory responses to bacteria when pattern recognition receptors bind pathogen-associated molecules such as lipopolysaccharide. Cells may also respond to tissue damage by sensing damage-associated molecules. Postpartum bacterial infections of the bovine uterus cause endometritis but the risk of disease is increased by tissue trauma triggered by dystocia. Animals that suffered dystocia had increased concentrations of inflammatory mediators IL-8, IL-1β and IL-1α in vaginal mucus 3 weeks postpartum, but they also had more bacteria than normal animals. Ex vivo organ cultures of endometrium, endometrial cells and peripheral blood monocytes did not generate inflammatory responses to prototypical damage molecules, HMGB1 or hyaluronan, or to necrotic cells; although they secreted IL-6 and IL-8 in a concentration-dependent manner when treated with IL-1α. However, necrotic endometrial cells did not accumulate intracellular IL-1α or release IL-1α, except when pre-treated with lipopolysaccharide or bacteria. Endometrial cell inflammatory responses to IL-1α were dependent on the cognate receptor IL-1R1, and the receptor adaptor protein MyD88, and the inflammatory response to IL-1α was independent of the response to lipopolysaccharide. Rather than a typical damage-associated molecule, IL-1α acts to scale the inflammatory response in recognition that there is a combination of pathogen challenge followed by endometrial cell damage. |
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| Item Description: |
This work was funded by Sheldon's UK Biotechnology and Biological Sciences Research Council grant (BBSRC, grant F005121/1).Healy was a PhD student supervised by Sheldon. |
| Start Page: |
7060 |