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The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates

Paula Row, John C Gray

Journal of Experimental Botany, Volume: 52, Issue: 354, Pages: 57 - 66

Swansea University Author: Paula Row

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DOI (Published version): 10.1093/jexbot/52.354.57

Abstract

In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethy...

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Published in: Journal of Experimental Botany
Published: 2001
Online Access: http://jxb.oxfordjournals.org/content/52/354/57.full.pdf
URI: https://cronfa.swan.ac.uk/Record/cronfa18376
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first_indexed 2014-09-11T01:59:10Z
last_indexed 2018-02-09T04:52:56Z
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spelling 2014-09-10T14:31:58.9994773 v2 18376 2014-09-10 The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates 99bb528b2f8fb62aabbdad101d53ba96 Paula Row Paula Row true false 2014-09-10 BMS In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethyl maleimide, diethyl pyrocarbonate, phenylglyoxal, 4,4'-di-isothiocyanatostilbene 2,2'-disulphonic acid (DIDS), dicyclohexylcarbodiimide (DCCD), and 1-ethyl- 3-dimethylaminopropylcarbodiimide (EDC) inhibited both import and formation of early import intermediates with precursor proteins by chloroplasts. This suggests that one or more of the binding components of the chloroplast protein import machinery contains functionally important solvent-exposed cysteine, histidine, arginine, and aspartate/glutamate residues, as well as functionally important lysine and aspartate/ glutamate residues in a hydrophobic environment. Journal Article Journal of Experimental Botany 52 354 57 66 31 12 2001 2001-12-31 10.1093/jexbot/52.354.57 http://jxb.oxfordjournals.org/content/52/354/57.full.pdf COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-09-10T14:31:58.9994773 2014-09-10T13:29:02.6181417 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Paula Row 1 John C Gray 2
title The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
spellingShingle The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
Paula Row
title_short The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
title_full The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
title_fullStr The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
title_full_unstemmed The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
title_sort The effect of amino acid-modifying reagents on chloroplast protein import and the formation of early import intermediates
author_id_str_mv 99bb528b2f8fb62aabbdad101d53ba96
author_id_fullname_str_mv 99bb528b2f8fb62aabbdad101d53ba96_***_Paula Row
author Paula Row
author2 Paula Row
John C Gray
format Journal article
container_title Journal of Experimental Botany
container_volume 52
container_issue 354
container_start_page 57
publishDate 2001
institution Swansea University
doi_str_mv 10.1093/jexbot/52.354.57
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url http://jxb.oxfordjournals.org/content/52/354/57.full.pdf
document_store_str 0
active_str 0
description In order to identify functionally important amino acid residues in the chloroplast protein import machinery, chloroplasts were preincubated with amino-acid-modifying reagents and then allowed to import or form early import intermediates with precursor proteins. Incubation of chloroplasts with N-ethyl maleimide, diethyl pyrocarbonate, phenylglyoxal, 4,4'-di-isothiocyanatostilbene 2,2'-disulphonic acid (DIDS), dicyclohexylcarbodiimide (DCCD), and 1-ethyl- 3-dimethylaminopropylcarbodiimide (EDC) inhibited both import and formation of early import intermediates with precursor proteins by chloroplasts. This suggests that one or more of the binding components of the chloroplast protein import machinery contains functionally important solvent-exposed cysteine, histidine, arginine, and aspartate/glutamate residues, as well as functionally important lysine and aspartate/ glutamate residues in a hydrophobic environment.
published_date 2001-12-31T03:21:32Z
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score 11.037056

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