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Evaluation of novel derivatisation reagents for the analysis of oxysterols

, William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

Biochemical and Biophysical Research Communications

Swansea University Authors: William Griffiths Orcid Logo, Yuqin Wang Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2014.01.173

Abstract

Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of...

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Published in: Biochemical and Biophysical Research Communications
Published: 2014
URI: https://cronfa.swan.ac.uk/Record/cronfa17816
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first_indexed 2014-04-15T01:30:03Z
last_indexed 2018-02-09T04:51:49Z
id cronfa17816
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spelling 2014-04-14T16:42:32.8701233 v2 17816 2014-04-14 Evaluation of novel derivatisation reagents for the analysis of oxysterols 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false c92729b58622f9fdf6a0e7d8f4ce5081 0000-0002-3063-3066 Yuqin Wang Yuqin Wang true false 2014-04-14 BMS Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of a strong chromophore and poor ionisation characteristics in mass spectrometry (MS). We have previously used enzyme-assisted derivatisation for sterol analysis (EADSA) to identify and quantitate oxysterols in biological samples. This technique relies on tagging sterols with the Girard P reagent to introduce a charged quaternary ammonium group. Here, we have compared several modified Girard-like reagents and show that the permanent charge is vital for efficient MSn fragmentation. However, we find that the reagent can be extended to include sites for potential stable isotope labels without a loss of performance Journal Article Biochemical and Biophysical Research Communications 31 12 2014 2014-12-31 10.1016/j.bbrc.2014.01.173 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2014-04-14T16:42:32.8701233 2014-04-14T16:42:32.8701233 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine 1 William Griffiths 0000-0002-4129-6616 2 Yuqin Wang 0000-0002-3063-3066 3
title Evaluation of novel derivatisation reagents for the analysis of oxysterols
spellingShingle Evaluation of novel derivatisation reagents for the analysis of oxysterols
William Griffiths
Yuqin Wang
title_short Evaluation of novel derivatisation reagents for the analysis of oxysterols
title_full Evaluation of novel derivatisation reagents for the analysis of oxysterols
title_fullStr Evaluation of novel derivatisation reagents for the analysis of oxysterols
title_full_unstemmed Evaluation of novel derivatisation reagents for the analysis of oxysterols
title_sort Evaluation of novel derivatisation reagents for the analysis of oxysterols
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
c92729b58622f9fdf6a0e7d8f4ce5081
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
c92729b58622f9fdf6a0e7d8f4ce5081_***_Yuqin Wang
author William Griffiths
Yuqin Wang
author2
William Griffiths
Yuqin Wang
format Journal article
container_title Biochemical and Biophysical Research Communications
publishDate 2014
institution Swansea University
doi_str_mv 10.1016/j.bbrc.2014.01.173
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
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description Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of a strong chromophore and poor ionisation characteristics in mass spectrometry (MS). We have previously used enzyme-assisted derivatisation for sterol analysis (EADSA) to identify and quantitate oxysterols in biological samples. This technique relies on tagging sterols with the Girard P reagent to introduce a charged quaternary ammonium group. Here, we have compared several modified Girard-like reagents and show that the permanent charge is vital for efficient MSn fragmentation. However, we find that the reagent can be extended to include sites for potential stable isotope labels without a loss of performance
published_date 2014-12-31T03:20:43Z
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score 11.037603

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