Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

Pascoe, Ben; Dams, Lucy; Wilkinson, Thomas; Harris, Llinos; Bodger, Owen; Mack, Dietrich; Davies, Angharad

doi:10.1371/journal.pone.0085998

Journal article 1537 views

Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

Ben Pascoe

, Lucy Dams, Thomas Wilkinson

, Llinos Harris

, Owen Bodger

, Dietrich Mack, Angharad Davies

PLoS ONE, Volume: 9, Issue: 2, Start page: e85998

Swansea University Authors: Ben Pascoe , Thomas Wilkinson , Llinos Harris , Owen Bodger , Angharad Davies

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DOI (Published version): 10.1371/journal.pone.0085998

Abstract

We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–...

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Published in:	PLoS ONE
Published:	2014
Online Access:	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998
URI:	https://cronfa.swan.ac.uk/Record/cronfa17301

first_indexed	2014-02-12T03:06:10Z
last_indexed	2018-02-09T04:50:49Z
id	cronfa17301
recordtype	SURis
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spelling	2016-04-19T23:51:39.3168921 v2 17301 2014-02-11 Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant 4660c0eb7e6bfd796cd749ae713ea558 0000-0001-6376-5121 Ben Pascoe Ben Pascoe true false 86cca6bf31bfe8572de27c1b441420d8 0000-0003-0397-6079 Thomas Wilkinson Thomas Wilkinson true false dc70f9d4badbbdb5d467fd321986d173 0000-0002-0295-3038 Llinos Harris Llinos Harris true false 8096440ab42b60a86e6aba678fe2695a 0000-0002-4022-9964 Owen Bodger Owen Bodger true false 62586459693e05b2e1063967e76883f1 0000-0003-4190-8894 Angharad Davies Angharad Davies true false 2014-02-11 We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–107 cfu/ml to ,10 cfu/ml as measured by the Most ProbableNumber method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR,remained around 106–107 cells/ml. Supplementing cultures with 25–50% spent medium resulted in a .600-fold increase inbacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spentsupernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence ofsmall coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) inactinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an importanthuman pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements inmanaging staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotictreatment. Journal Article PLoS ONE 9 2 e85998 Staphylococcus aureus; dormancy; bacterial infection; chronic infection 11 2 2014 2014-02-11 10.1371/journal.pone.0085998 http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998 COLLEGE NANME COLLEGE CODE Swansea University 2016-04-19T23:51:39.3168921 2014-02-11T23:37:13.9807140 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Ben Pascoe 0000-0001-6376-5121 1 Lucy Dams 2 Thomas Wilkinson 0000-0003-0397-6079 3 Llinos Harris 0000-0002-0295-3038 4 Owen Bodger 0000-0002-4022-9964 5 Dietrich Mack 6 Angharad Davies 0000-0003-4190-8894 7
title	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
spellingShingle	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant Ben Pascoe Thomas Wilkinson Llinos Harris Owen Bodger Angharad Davies
title_short	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_full	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_fullStr	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_full_unstemmed	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
title_sort	Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant
author_id_str_mv	4660c0eb7e6bfd796cd749ae713ea558 86cca6bf31bfe8572de27c1b441420d8 dc70f9d4badbbdb5d467fd321986d173 8096440ab42b60a86e6aba678fe2695a 62586459693e05b2e1063967e76883f1
author_id_fullname_str_mv	4660c0eb7e6bfd796cd749ae713ea558_*_Ben Pascoe 86cca6bf31bfe8572de27c1b441420d8__Thomas Wilkinson dc70f9d4badbbdb5d467fd321986d173__Llinos Harris 8096440ab42b60a86e6aba678fe2695a__Owen Bodger 62586459693e05b2e1063967e76883f1_**_Angharad Davies
author	Ben Pascoe Thomas Wilkinson Llinos Harris Owen Bodger Angharad Davies
author2	Ben Pascoe Lucy Dams Thomas Wilkinson Llinos Harris Owen Bodger Dietrich Mack Angharad Davies
format	Journal article
container_title	PLoS ONE
container_volume	9
container_issue	2
container_start_page	e85998
publishDate	2014
institution	Swansea University
doi_str_mv	10.1371/journal.pone.0085998
college_str	Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id	facultyofmedicinehealthandlifesciences
hierarchy_top_title	Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id	facultyofmedicinehealthandlifesciences
hierarchy_parent_title	Faculty of Medicine, Health and Life Sciences
department_str	Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
url	http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0085998
document_store_str	0
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description	We describe the first in vitro model of dormancy in Staphylococcus aureus, showing that cells are generated which can beresuscitated by addition of spent medium supernatant taken from cultures of the same organism. Over 30 days, culturablecounts in dormant cultures of S. aureus SH1000 fell from 106–107 cfu/ml to ,10 cfu/ml as measured by the Most ProbableNumber method in liquid culture, while total counts as determined by microscopy, and supported by data from RT-qPCR,remained around 106–107 cells/ml. Supplementing cultures with 25–50% spent medium resulted in a .600-fold increase inbacterial growth. Resuscitation was a specific effect, greatly reduced by boiling or addition of trypsin to the spentsupernatant. Supernatant also effected a reduction in lag phase of dormant cultures. SEM demonstrated the presence ofsmall coccoid cells in dormant cultures. The results are similar to those seen with resuscitation promoting factors (Rpfs) inactinobacteria. This is the first time resuscitation has been demonstrated in Staphylococcus aureus, which is an importanthuman pathogen. A better understanding of control and reactivation of dormant cells could lead to major improvements inmanaging staphylococcal infections; resuscitation could be an important step in restoring susceptibility to antibiotictreatment.
published_date	2014-02-11T06:32:40Z
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score	11.04748

Dormant Cells of Staphylococcus aureus Are Resuscitated by Spent Culture Supernatant

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