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In vitro growth factor-induced bio engineering of mature articular cartilage

Ilyas Khan Orcid Logo, Lewis Francis Orcid Logo, Peter S Theobald, Stefano Perni, Robert D Young, Polina Prokopovich, R. Steven Conlan, Charles W Archer, Steve Conlan Orcid Logo

Biomaterials, Volume: 34, Issue: 5, Pages: 1478 - 1487

Swansea University Authors: Ilyas Khan Orcid Logo, Lewis Francis Orcid Logo, Steve Conlan Orcid Logo

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DOI (Published version): 10.1016/j.biomaterials.2012.09.076

Abstract

Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual ma...

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Published in: Biomaterials
ISSN: 0142-9612
Published: 2013
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URI: https://cronfa.swan.ac.uk/Record/cronfa13945
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Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual maturity. The inability to induce maturation in biomaterial constructs designed for cartilage repair has been cited as a major cause for their failure in producing persistent cell-based repair of joint lesions. The combination of growth factors FGF2 and TGF&#x3B2;1 induces accelerated articular cartilage maturation in vitro such that many molecular and morphological characteristics of tissue maturation are observable. We hypothesised that experimental growth factor-induced maturation of immature cartilage would result in a biophysical and biochemical composition consistent with a mature phenotype. Using native immature and mature cartilage as reference, we observed that growth factor-treated immature cartilages displayed increased nano-compressive stiffness, decreased surface adhesion, decreased water content, increased collagen content and smoother surfaces, correlating with a convergence to the mature cartilage phenotype. Furthermore, increased gene expression of surface structural protein collagen type I in growth factor-treated explants compared to reference cartilages demonstrates that they are still in the dynamic phase of the postnatal developmental transition. These data provide a basis for understanding the regulation of postnatal maturation of articular cartilage and the application of growth factor-induced maturation in vitro and in vivo in order to repair and regenerate cartilage defects.</abstract><type>Journal Article</type><journal>Biomaterials</journal><volume>34</volume><journalNumber>5</journalNumber><paginationStart>1478</paginationStart><paginationEnd>1487</paginationEnd><publisher/><issnPrint>0142-9612</issnPrint><keywords>Cartilage, Maturation, FGF2, TGFb1, biomechanical</keywords><publishedDay>28</publishedDay><publishedMonth>2</publishedMonth><publishedYear>2013</publishedYear><publishedDate>2013-02-28</publishedDate><doi>10.1016/j.biomaterials.2012.09.076</doi><url/><notes>The work described in this study has led to an award of a grant of &#xA3;68,000 (18 months) to Dr I.M.Khan from Orthopaedic Research UK which is due to start in March 2013.</notes><college>COLLEGE NANME</college><department>Biomedical Sciences</department><CollegeCode>COLLEGE CODE</CollegeCode><DepartmentCode>BMS</DepartmentCode><institution>Swansea University</institution><apcterm/><lastEdited>2019-07-10T12:13:19.7789479</lastEdited><Created>2013-01-21T16:52:44.5036679</Created><path><level id="1">Faculty of Medicine, Health and Life Sciences</level><level id="2">Swansea University Medical School - Medicine</level></path><authors><author><firstname>Ilyas</firstname><surname>Khan</surname><orcid>0000-0002-3886-1987</orcid><order>1</order></author><author><firstname>Lewis</firstname><surname>Francis</surname><orcid>0000-0002-7803-7714</orcid><order>2</order></author><author><firstname>Peter S</firstname><surname>Theobald</surname><order>3</order></author><author><firstname>Stefano</firstname><surname>Perni</surname><order>4</order></author><author><firstname>Robert D</firstname><surname>Young</surname><order>5</order></author><author><firstname>Polina</firstname><surname>Prokopovich</surname><order>6</order></author><author><firstname>R. Steven</firstname><surname>Conlan</surname><order>7</order></author><author><firstname>Charles W</firstname><surname>Archer</surname><order>8</order></author><author><firstname>Steve</firstname><surname>Conlan</surname><orcid>0000-0002-2562-3461</orcid><order>9</order></author></authors><documents><document><filename>0013945-19062019155018.pdf</filename><originalFilename>13945.pdf</originalFilename><uploaded>2019-06-19T15:50:18.2900000</uploaded><type>Output</type><contentLength>1283267</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-06-18T00:00:00.0000000</embargoDate><documentNotes>Released under the terms of a Creative Commons Attribution License (CC-BY).</documentNotes><copyrightCorrect>true</copyrightCorrect><language>eng</language></document><document><filename>0013945-13062019160716.pdf</filename><originalFilename>InVitroGrowthFactorInducedBioEngineeringOfMatureArticularCartilage.pdf</originalFilename><uploaded>2019-06-13T16:07:16.2870000</uploaded><type>Output</type><contentLength>905321</contentLength><contentType>application/pdf</contentType><version>Version of Record</version><cronfaStatus>true</cronfaStatus><embargoDate>2019-06-13T00:00:00.0000000</embargoDate><copyrightCorrect>true</copyrightCorrect><language>eng</language></document></documents><OutputDurs/></rfc1807>
spelling 2019-07-10T12:13:19.7789479 v2 13945 2013-01-21 In vitro growth factor-induced bio engineering of mature articular cartilage 2536d955ff70e7b77063a8efe9103161 0000-0002-3886-1987 Ilyas Khan Ilyas Khan true false 10f61f9c1248951c1a33f6a89498f37d 0000-0002-7803-7714 Lewis Francis Lewis Francis true false 0bb6bd247e32fb4249de62c0013b51cb 0000-0002-2562-3461 Steve Conlan Steve Conlan true false 2013-01-21 BMS Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual maturity. The inability to induce maturation in biomaterial constructs designed for cartilage repair has been cited as a major cause for their failure in producing persistent cell-based repair of joint lesions. The combination of growth factors FGF2 and TGFβ1 induces accelerated articular cartilage maturation in vitro such that many molecular and morphological characteristics of tissue maturation are observable. We hypothesised that experimental growth factor-induced maturation of immature cartilage would result in a biophysical and biochemical composition consistent with a mature phenotype. Using native immature and mature cartilage as reference, we observed that growth factor-treated immature cartilages displayed increased nano-compressive stiffness, decreased surface adhesion, decreased water content, increased collagen content and smoother surfaces, correlating with a convergence to the mature cartilage phenotype. Furthermore, increased gene expression of surface structural protein collagen type I in growth factor-treated explants compared to reference cartilages demonstrates that they are still in the dynamic phase of the postnatal developmental transition. These data provide a basis for understanding the regulation of postnatal maturation of articular cartilage and the application of growth factor-induced maturation in vitro and in vivo in order to repair and regenerate cartilage defects. Journal Article Biomaterials 34 5 1478 1487 0142-9612 Cartilage, Maturation, FGF2, TGFb1, biomechanical 28 2 2013 2013-02-28 10.1016/j.biomaterials.2012.09.076 The work described in this study has led to an award of a grant of £68,000 (18 months) to Dr I.M.Khan from Orthopaedic Research UK which is due to start in March 2013. COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2019-07-10T12:13:19.7789479 2013-01-21T16:52:44.5036679 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Ilyas Khan 0000-0002-3886-1987 1 Lewis Francis 0000-0002-7803-7714 2 Peter S Theobald 3 Stefano Perni 4 Robert D Young 5 Polina Prokopovich 6 R. Steven Conlan 7 Charles W Archer 8 Steve Conlan 0000-0002-2562-3461 9 0013945-19062019155018.pdf 13945.pdf 2019-06-19T15:50:18.2900000 Output 1283267 application/pdf Version of Record true 2019-06-18T00:00:00.0000000 Released under the terms of a Creative Commons Attribution License (CC-BY). true eng 0013945-13062019160716.pdf InVitroGrowthFactorInducedBioEngineeringOfMatureArticularCartilage.pdf 2019-06-13T16:07:16.2870000 Output 905321 application/pdf Version of Record true 2019-06-13T00:00:00.0000000 true eng
title In vitro growth factor-induced bio engineering of mature articular cartilage
spellingShingle In vitro growth factor-induced bio engineering of mature articular cartilage
Ilyas Khan
Lewis Francis
Steve Conlan
title_short In vitro growth factor-induced bio engineering of mature articular cartilage
title_full In vitro growth factor-induced bio engineering of mature articular cartilage
title_fullStr In vitro growth factor-induced bio engineering of mature articular cartilage
title_full_unstemmed In vitro growth factor-induced bio engineering of mature articular cartilage
title_sort In vitro growth factor-induced bio engineering of mature articular cartilage
author_id_str_mv 2536d955ff70e7b77063a8efe9103161
10f61f9c1248951c1a33f6a89498f37d
0bb6bd247e32fb4249de62c0013b51cb
author_id_fullname_str_mv 2536d955ff70e7b77063a8efe9103161_***_Ilyas Khan
10f61f9c1248951c1a33f6a89498f37d_***_Lewis Francis
0bb6bd247e32fb4249de62c0013b51cb_***_Steve Conlan
author Ilyas Khan
Lewis Francis
Steve Conlan
author2 Ilyas Khan
Lewis Francis
Peter S Theobald
Stefano Perni
Robert D Young
Polina Prokopovich
R. Steven Conlan
Charles W Archer
Steve Conlan
format Journal article
container_title Biomaterials
container_volume 34
container_issue 5
container_start_page 1478
publishDate 2013
institution Swansea University
issn 0142-9612
doi_str_mv 10.1016/j.biomaterials.2012.09.076
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 1
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description Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Maturation involves gross morphological changes that occur through a process of synchronised growth and resorption of cartilage and generally ends at sexual maturity. The inability to induce maturation in biomaterial constructs designed for cartilage repair has been cited as a major cause for their failure in producing persistent cell-based repair of joint lesions. The combination of growth factors FGF2 and TGFβ1 induces accelerated articular cartilage maturation in vitro such that many molecular and morphological characteristics of tissue maturation are observable. We hypothesised that experimental growth factor-induced maturation of immature cartilage would result in a biophysical and biochemical composition consistent with a mature phenotype. Using native immature and mature cartilage as reference, we observed that growth factor-treated immature cartilages displayed increased nano-compressive stiffness, decreased surface adhesion, decreased water content, increased collagen content and smoother surfaces, correlating with a convergence to the mature cartilage phenotype. Furthermore, increased gene expression of surface structural protein collagen type I in growth factor-treated explants compared to reference cartilages demonstrates that they are still in the dynamic phase of the postnatal developmental transition. These data provide a basis for understanding the regulation of postnatal maturation of articular cartilage and the application of growth factor-induced maturation in vitro and in vivo in order to repair and regenerate cartilage defects.
published_date 2013-02-28T03:15:57Z
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score 11.037581

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