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Raman spectroscopy for rapid discrimination of Staphylococcus epidermidis clones related to medical device-associated infections

O Samek, H.H Telle, L.G Harris, M Bloomfield, D Mack, Helmut Telle, Llinos Harris Orcid Logo

Laser Physics Letters, Volume: 5, Issue: 6, Pages: 465 - 470

Swansea University Authors: Helmut Telle, Llinos Harris Orcid Logo

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DOI (Published version): 10.1002/lapl.200810011

Abstract

We report on the potential application of Raman spectroscopy for the fast typing of Staphylococcus epidermidis (S. epidermidis) strains related to medical device-associated infections. In this study bacterial colonies were directly probed on culture plates and Raman spectra were recorded from volume...

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Published in: Laser Physics Letters
ISSN: 1612-2011 1612-202X
Published: 2008
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URI: https://cronfa.swan.ac.uk/Record/cronfa1162
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Abstract: We report on the potential application of Raman spectroscopy for the fast typing of Staphylococcus epidermidis (S. epidermidis) strains related to medical device-associated infections. In this study bacterial colonies were directly probed on culture plates and Raman spectra were recorded from volumes containing approximately 10 bacteria. The spectra contain information on the molecular composition of the whole bacteria, such as fatty acids, carbohydrates, proteins and nucleic acids, DNA as well as RNA. We demonstrate the potential to discriminate different S. epidermidis clones, even after only short Raman exposure/collection times.
Item Description: a. The author made a substantial contribution to the conception and design of the study; to the organisation of the conduct of the study; and to analysis and interpretation of study data.
College: Faculty of Science and Engineering
Issue: 6
Start Page: 465
End Page: 470