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Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat

Rosemary Smyth, Catherine S Lane, Rukhsana Ashiq, John A Turton, Christopher J Clarke, Theo O Dare, Malcolm J York, William Griffiths Orcid Logo, Michael R Munday

Cell Biology and Toxicology, Volume: 25, Issue: 5, Pages: 499 - 512

Swansea University Author: William Griffiths Orcid Logo

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DOI (Published version): 10.1007/s10565-008-9104-8

Abstract

Proteomic techniques such as two-dimensional gel electrophoresis (2-DGE) and mass spectrometry have become important tools for the identification of novel biomarkers of toxicity and disease. Ideally, such biomarkers need to be sensitive and organ specific, but, recently, it has become apparent that...

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Published in: Cell Biology and Toxicology
ISSN: 0742-2091 1573-6822
Published: 2009
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URI: https://cronfa.swan.ac.uk/Record/cronfa10952
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first_indexed 2013-07-23T12:04:41Z
last_indexed 2018-02-09T04:40:14Z
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spelling 2011-10-01T00:00:00.0000000 v2 10952 2012-06-05 Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-06-05 BMS Proteomic techniques such as two-dimensional gel electrophoresis (2-DGE) and mass spectrometry have become important tools for the identification of novel biomarkers of toxicity and disease. Ideally, such biomarkers need to be sensitive and organ specific, but, recently, it has become apparent that it would be an additional benefit to be able to measure biomarkers in samples obtained using non-invasive methods. The present study is concerned with the identification of novel urinary markers of hepatic fibrosis. In a carbon-tetrachloride-induced liver fibrosis rat model, analysis of urine by 2-DGE revealed an increase in the concentration of a number of proteins in animals with hepatic fibrosis. Using in-gel trypsin digest and nano-scale liquid chromatography combined with electrospray ionisation tandem mass spectrometry, protein spots were identified as copper/zinc superoxide dismutase, d-dopachrome tautomerase, beta-2-microglobulin and neutrophil gelatinase associated lipocalin. These proteins are known to have important roles in the inflammatory response. Journal Article Cell Biology and Toxicology 25 5 499 512 0742-2091 1573-6822 31 12 2009 2009-12-31 10.1007/s10565-008-9104-8 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-06-05T17:04:53.4085450 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine Rosemary Smyth 1 Catherine S Lane 2 Rukhsana Ashiq 3 John A Turton 4 Christopher J Clarke 5 Theo O Dare 6 Malcolm J York 7 William Griffiths 0000-0002-4129-6616 8 Michael R Munday 9
title Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
spellingShingle Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
William Griffiths
title_short Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
title_full Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
title_fullStr Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
title_full_unstemmed Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
title_sort Proteomic investigation of urinary markers of carbon-tetrachloride-induced hepatic fibrosis in the Hanover Wistar rat
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 Rosemary Smyth
Catherine S Lane
Rukhsana Ashiq
John A Turton
Christopher J Clarke
Theo O Dare
Malcolm J York
William Griffiths
Michael R Munday
format Journal article
container_title Cell Biology and Toxicology
container_volume 25
container_issue 5
container_start_page 499
publishDate 2009
institution Swansea University
issn 0742-2091
1573-6822
doi_str_mv 10.1007/s10565-008-9104-8
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Proteomic techniques such as two-dimensional gel electrophoresis (2-DGE) and mass spectrometry have become important tools for the identification of novel biomarkers of toxicity and disease. Ideally, such biomarkers need to be sensitive and organ specific, but, recently, it has become apparent that it would be an additional benefit to be able to measure biomarkers in samples obtained using non-invasive methods. The present study is concerned with the identification of novel urinary markers of hepatic fibrosis. In a carbon-tetrachloride-induced liver fibrosis rat model, analysis of urine by 2-DGE revealed an increase in the concentration of a number of proteins in animals with hepatic fibrosis. Using in-gel trypsin digest and nano-scale liquid chromatography combined with electrospray ionisation tandem mass spectrometry, protein spots were identified as copper/zinc superoxide dismutase, d-dopachrome tautomerase, beta-2-microglobulin and neutrophil gelatinase associated lipocalin. These proteins are known to have important roles in the inflammatory response.
published_date 2009-12-31T03:12:30Z
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score 11.037144

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