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General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry

H.L.J Makin, J.W Honour, C.H.L Shackleton, William Griffiths Orcid Logo

Steroid Analysis,

Swansea University Author: William Griffiths Orcid Logo

Abstract

Steroids consist of an essentially lipophilic (or hydrophobic, non-polar) cyclopentanoperhydrophenanthrene nucleus modified on the periphery of the nucleus or on the side chain by the addition of hydrophilic (or lipophobic, polar) groups. Although steroids are widely distributed in nature and many t...

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Published in: Steroid Analysis,
Published: Springer Science 2010
URI: https://cronfa.swan.ac.uk/Record/cronfa10942
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spelling 2011-10-01T00:00:00.0000000 v2 10942 2012-06-05 General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-06-05 MEDS Steroids consist of an essentially lipophilic (or hydrophobic, non-polar) cyclopentanoperhydrophenanthrene nucleus modified on the periphery of the nucleus or on the side chain by the addition of hydrophilic (or lipophobic, polar) groups. Although steroids are widely distributed in nature and many thousands have been synthesised in the laboratories of pharmaceutical and chemical organisations, this chapter concentrates primarily on the methodology for the analysis of steroids of biological importance to human subjects and in particular on the methods for the analysis of the very low concentrations of steroids found in human biological tissues or formed during in vitro or in vivo studies. This does not, however, imply that the techniques discussed here may not find applicability in other areas of steroid analysis. This chapter neither discusses specifically the saturation analysis techniques including immunoassay-radioimmunoassay (RIA), enzymeimmunoassay (EIA), which are explained in Chapter 4, nor the analysis of cardenolides, sapogenins, alkaloids, brassinosteroids or ecdysteroids, which present their own analytical challenges but are of less interest in a clinical context. Further details on basic principles of mass spectrometry (MS) are discussed in Chapter 2. Book chapter Steroid Analysis, Springer Science 31 12 2010 2010-12-31 COLLEGE NANME Medical School COLLEGE CODE MEDS Swansea University 2011-10-01T00:00:00.0000000 2012-06-05T16:05:35.0365572 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine H.L.J Makin 1 J.W Honour 2 C.H.L Shackleton 3 William Griffiths 0000-0002-4129-6616 4
title General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
spellingShingle General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
William Griffiths
title_short General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
title_full General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
title_fullStr General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
title_full_unstemmed General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
title_sort General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 H.L.J Makin
J.W Honour
C.H.L Shackleton
William Griffiths
format Book chapter
container_title Steroid Analysis,
publishDate 2010
institution Swansea University
publisher Springer Science
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Steroids consist of an essentially lipophilic (or hydrophobic, non-polar) cyclopentanoperhydrophenanthrene nucleus modified on the periphery of the nucleus or on the side chain by the addition of hydrophilic (or lipophobic, polar) groups. Although steroids are widely distributed in nature and many thousands have been synthesised in the laboratories of pharmaceutical and chemical organisations, this chapter concentrates primarily on the methodology for the analysis of steroids of biological importance to human subjects and in particular on the methods for the analysis of the very low concentrations of steroids found in human biological tissues or formed during in vitro or in vivo studies. This does not, however, imply that the techniques discussed here may not find applicability in other areas of steroid analysis. This chapter neither discusses specifically the saturation analysis techniques including immunoassay-radioimmunoassay (RIA), enzymeimmunoassay (EIA), which are explained in Chapter 4, nor the analysis of cardenolides, sapogenins, alkaloids, brassinosteroids or ecdysteroids, which present their own analytical challenges but are of less interest in a clinical context. Further details on basic principles of mass spectrometry (MS) are discussed in Chapter 2.
published_date 2010-12-31T06:20:10Z
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score 11.064479

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