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Analytical strategies for characterization of bile acid and oxysterol metabolomes

William Griffiths Orcid Logo, Jan Sjövall

Biochemical and Biophysical Research Communications, Volume: 396, Issue: 1, Pages: 80 - 84

Swansea University Author: William Griffiths Orcid Logo

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DOI (Published version): 10.1016/j.bbrc.2010.02.149

Abstract

Cholesterol is the precursor of many compounds with functions in the physiology and metabolism of the organism. Methods for the multicomponent analysis of these compounds and their metabolites (metabolomics) are needed to improve our understanding of their roles in different species, organs, cells a...

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Published in: Biochemical and Biophysical Research Communications
ISSN: 0006-291X
Published: 2010
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URI: https://cronfa.swan.ac.uk/Record/cronfa10782
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last_indexed 2018-02-09T04:39:53Z
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spelling 2011-10-01T00:00:00.0000000 v2 10782 2012-05-22 Analytical strategies for characterization of bile acid and oxysterol metabolomes 3316b1d1b524be1831790933eed1c26e 0000-0002-4129-6616 William Griffiths William Griffiths true false 2012-05-22 BMS Cholesterol is the precursor of many compounds with functions in the physiology and metabolism of the organism. Methods for the multicomponent analysis of these compounds and their metabolites (metabolomics) are needed to improve our understanding of their roles in different species, organs, cells and metabolic situations and to clarify structure/activity relationships. This review discusses methods based on combinations of ion exchange and reversed-phase separations for sample preparation with derivatization and “charge-tagging” for chromatography-mass spectrometry in qualitative and quantitative characterizations of oxysterol, bile alcohol, bile acid, and steroid hormone metabolomes. Advantages, disadvantages and potential improvements for high-throughput applications are briefly discussed. Other Biochemical and Biophysical Research Communications 396 1 80 84 0006-291X 31 12 2010 2010-12-31 10.1016/j.bbrc.2010.02.149 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2011-10-01T00:00:00.0000000 2012-05-22T16:34:23.4074005 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine William Griffiths 0000-0002-4129-6616 1 Jan Sjövall 2
title Analytical strategies for characterization of bile acid and oxysterol metabolomes
spellingShingle Analytical strategies for characterization of bile acid and oxysterol metabolomes
William Griffiths
title_short Analytical strategies for characterization of bile acid and oxysterol metabolomes
title_full Analytical strategies for characterization of bile acid and oxysterol metabolomes
title_fullStr Analytical strategies for characterization of bile acid and oxysterol metabolomes
title_full_unstemmed Analytical strategies for characterization of bile acid and oxysterol metabolomes
title_sort Analytical strategies for characterization of bile acid and oxysterol metabolomes
author_id_str_mv 3316b1d1b524be1831790933eed1c26e
author_id_fullname_str_mv 3316b1d1b524be1831790933eed1c26e_***_William Griffiths
author William Griffiths
author2 William Griffiths
Jan Sjövall
format Other
container_title Biochemical and Biophysical Research Communications
container_volume 396
container_issue 1
container_start_page 80
publishDate 2010
institution Swansea University
issn 0006-291X
doi_str_mv 10.1016/j.bbrc.2010.02.149
college_str Faculty of Medicine, Health and Life Sciences
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hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
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description Cholesterol is the precursor of many compounds with functions in the physiology and metabolism of the organism. Methods for the multicomponent analysis of these compounds and their metabolites (metabolomics) are needed to improve our understanding of their roles in different species, organs, cells and metabolic situations and to clarify structure/activity relationships. This review discusses methods based on combinations of ion exchange and reversed-phase separations for sample preparation with derivatization and “charge-tagging” for chromatography-mass spectrometry in qualitative and quantitative characterizations of oxysterol, bile alcohol, bile acid, and steroid hormone metabolomes. Advantages, disadvantages and potential improvements for high-throughput applications are briefly discussed.
published_date 2010-12-31T03:12:16Z
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