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Triclosan anatagonizes fluconazole activity against candida albicans

J Higgins, E Pinjon, HN Oltean, TC White, Steven Kelly Orcid Logo, CM Martel, DJ Sulivan, DC Coleman, GP Moran, Claire Hull

Journal of Dental Research, Volume: 91, Issue: 1, Pages: 65 - 70

Swansea University Authors: Steven Kelly Orcid Logo, Claire Hull

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DOI (Published version): 10.1177/0022034511425046

Abstract

Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the acti...

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Published in: Journal of Dental Research
Published: 2012
URI: https://cronfa.swan.ac.uk/Record/cronfa10166
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spelling 2021-10-26T17:00:59.9613887 v2 10166 2012-03-21 Triclosan anatagonizes fluconazole activity against candida albicans b17cebaf09b4d737b9378a3581e3de93 0000-0001-7991-5040 Steven Kelly Steven Kelly true false 6c495cb489b67bc9bb3f746975c4ff19 Claire Hull Claire Hull true false 2012-03-21 BMS Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1 Delta and cdr2 Delta strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1 Delta strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes. Journal Article Journal of Dental Research 91 1 65 70 triclosan; fluconazole; antagonism; Candida albicans; EFG1; hyphae 31 12 2012 2012-12-31 10.1177/0022034511425046 COLLEGE NANME Biomedical Sciences COLLEGE CODE BMS Swansea University 2021-10-26T17:00:59.9613887 2012-03-21T16:17:17.0000000 Faculty of Medicine, Health and Life Sciences Swansea University Medical School - Medicine J Higgins 1 E Pinjon 2 HN Oltean 3 TC White 4 Steven Kelly 0000-0001-7991-5040 5 CM Martel 6 DJ Sulivan 7 DC Coleman 8 GP Moran 9 Claire Hull 10
title Triclosan anatagonizes fluconazole activity against candida albicans
spellingShingle Triclosan anatagonizes fluconazole activity against candida albicans
Steven Kelly
Claire Hull
title_short Triclosan anatagonizes fluconazole activity against candida albicans
title_full Triclosan anatagonizes fluconazole activity against candida albicans
title_fullStr Triclosan anatagonizes fluconazole activity against candida albicans
title_full_unstemmed Triclosan anatagonizes fluconazole activity against candida albicans
title_sort Triclosan anatagonizes fluconazole activity against candida albicans
author_id_str_mv b17cebaf09b4d737b9378a3581e3de93
6c495cb489b67bc9bb3f746975c4ff19
author_id_fullname_str_mv b17cebaf09b4d737b9378a3581e3de93_***_Steven Kelly
6c495cb489b67bc9bb3f746975c4ff19_***_Claire Hull
author Steven Kelly
Claire Hull
author2 J Higgins
E Pinjon
HN Oltean
TC White
Steven Kelly
CM Martel
DJ Sulivan
DC Coleman
GP Moran
Claire Hull
format Journal article
container_title Journal of Dental Research
container_volume 91
container_issue 1
container_start_page 65
publishDate 2012
institution Swansea University
doi_str_mv 10.1177/0022034511425046
college_str Faculty of Medicine, Health and Life Sciences
hierarchytype
hierarchy_top_id facultyofmedicinehealthandlifesciences
hierarchy_top_title Faculty of Medicine, Health and Life Sciences
hierarchy_parent_id facultyofmedicinehealthandlifesciences
hierarchy_parent_title Faculty of Medicine, Health and Life Sciences
department_str Swansea University Medical School - Medicine{{{_:::_}}}Faculty of Medicine, Health and Life Sciences{{{_:::_}}}Swansea University Medical School - Medicine
document_store_str 0
active_str 0
description Triclosan is a broad-spectrum antimicrobial compound commonly used in oral hygiene products. Investigation of its activity against Candida albicans showed that triclosan was fungicidal at concentrations of 16 mg/L. However, at subinhibitory concentrations (0.5-2 mg/L), triclosan antagonized the activity of fluconazole. Although triclosan induced CDR1 expression in C. albicans, antagonism was still observed in cdr1 Delta and cdr2 Delta strains. Triclosan did not affect fluconazole uptake or alter total membrane sterol content, but did induce the expression of FAS1 and FAS2, indicating that its mode of action may involve inhibition of fatty acid synthesis, as it does in prokaryotes. However, FAS2 mutants did not exhibit increased susceptibility to triclosan, and overexpression of both FAS1 and FAS2 alleles did not alter triclosan susceptibility. Unexpectedly, the antagonistic effect was specific for C. albicans under hypha-inducing conditions and was absent in the non-filamentous efg1 Delta strain. This antagonism may be due to the membranotropic activity of triclosan and the unique composition of hyphal membranes.
published_date 2012-12-31T03:11:32Z
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score 11.017731

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